Pooled Libraries
Mouse Subpooled CRISPRa-v2 Libraries

Mouse Subpooled CRISPRa-v2 Libraries
(Pooled Libraries #83998, #83999, #84000, #84001, #84002, #84003, #84004)

Purpose
Version 2 - Mouse subpooled CRISPRa libraries for activation of gene transcription. Available as 5 gRNAs per gene.
Vector Backbone
pCRISPRia-v2 - does not express Cas9

Depositing Labs
Jonathan Weissman
Publication
Horlbeck et al Elife. 2016 Sep 23;5. pii: e19760. doi: 10.7554/eLife.19760 ( How to cite )

Ordering

Item	Catalog #	Description	Quantity
Pooled Library	83998	Kinases, Phosphatases, and Drug Targets - gRNA pooled library^†	1	$400	Add to Cart
Pooled Library	83999	Cancer and Apoptosis - gRNA pooled library^†	1	$300	Add to Cart
Pooled Library	84000	Stress and Proteostasis - gRNA pooled library^†	1	$300	Add to Cart
Pooled Library	84001	Mitochondria, Trafficking, and Motility - gRNA pooled library^†	1	$300	Add to Cart
Pooled Library	84002	Gene expression - gRNA pooled library^†	1	$400	Add to Cart
Pooled Library	84003	Membrane Proteins - gRNA pooled library^†	1	$400	Add to Cart
Pooled Library	84004	Unassigned - gRNA pooled library^†	1	$300	Add to Cart

Available to Academic and Nonprofits Only

^† A Cas9 plasmid is NOT included with this item and will have to be ordered separately. Can be used with SunTag-VP64 constructs (#60903 and #60904) or otherwise used with cell lines already expressing activator dCas9.
If you would like to receive all seven sublibraries in a single order, please email [email protected] for pricing (if you use a distributor in your country, please contact your distributor directly for pricing).

Library Details

Pooled Library	Genes Targeted	Targeting gRNAs	Control gRNAs
83998	2268	12,090	250
83999	2856	15,195	280
84000	2796	14,660	290
84001	2099	11,040	250
84002	1915	10,080	250
84003	2104	10,975	250
84004	5901	30,895	600

Species
Mouse
Lentiviral Generation
3rd

Library Shipping

These libraries are delivered as suspended DNA in microcentrifuge tubes on blue ice. The tube's contents will not necessarily be frozen. For best results, minimize freeze-thaw cycles.

Volume
20 µL
Concentration
12.5 ng/µL

Resource Information

Protocols
- Illumina Sequencing Sample Preparation (752 KB)
- Liquid Culture Re-Amplification Protocol (71 KB)
- Cell Line Creation and Validation Primer (125 KB)
- CRISPRi/a Custom Library Cloning Protocol (92 KB)
- For more links and protocols, please see the Weismann lab's CRISPRi/a screening materials page.
Depositor Data
- Guide Sequences (16 MB)
- Mouse Subpool Gene List (365 KB)
Terms and Licenses
- UBMTA
- Evrogen Limited Use Label License for FPs

Depositor Comments

In addition to SunTag-VP64 constructs (#60903 and #60904), another dCas9-containing construct that can be used with these libraries is dCas9-VPR (#63798).

The data analysis pipeline for analyzing sequencing data is available on GitHub.

How to cite this pooled library ( Back to top )

These pooled libraries were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the pooled libraries were described, and include Addgene in the Materials and Methods of your future publications.

Example for your Materials & Methods section:
mCRISPRa-v2 subpooled library was a gift from Jonathan Weissman (Addgene ID #)
For your References section:
Compact and highly active next-generation libraries for CRISPR-mediated gene repression and activation . Horlbeck MA, Gilbert LA, Villalta JE, Adamson B, Pak RA, Chen Y, Fields AP, Park CY, Corn JE, Kampmann M, Weissman JS Elife. 2016 Sep 23;5. pii: e19760. doi: 10.7554/eLife.19760. PubMed 27661255

Mouse Subpooled CRISPRa-v2 Libraries (Pooled Libraries #83998, #83999, #84000, #84001, #84002, #84003, #84004)