Bassik Human CRISPR Knockout Library
(Pooled Libraries #101926, #101927, #101928, #101929, #101930, #101931, #101932, #101933, #101934)
This lentiviral genome-scale CRISPR library targets all ~20,500 protein-coding genes in the human genome. The library is divided into 9 sublibraries defined by biological categories of genes, each with their own negative controls.
This library includes safe-targeting controls, gRNAs which target the genome but are not expected to disrupt gene function, in addition to nontargeting controls. The gRNAs vary in length from 17-20 bp, with the truncated gRNAs (17 or 18 bp) having less off-target activity.
pMCB320 - does not express Cas9
|Item||Catalog #||Description||Quantity||Price (USD)|
|Pooled Library||101926||Human CRISPR Deletion Library - Apoptosis and cancer†||1||$500||Add to Cart|
|Pooled Library||101927||Human CRISPR Deletion Library - Drug targets, kinases, phosphatases†||1||$500||Add to Cart|
|Pooled Library||101928||Human CRISPR Deletion Library - Gene expression†||1||$500||Add to Cart|
|Pooled Library||101929||Human CRISPR Deletion Library - Membrane proteins†||1||$500||Add to Cart|
|Pooled Library||101930||Human CRISPR Deletion Library - Proteostasis†||1||$500||Add to Cart|
|Pooled Library||101931||Human CRISPR Deletion Library - Trafficking, mitochondrial, motility†||1||$500||Add to Cart|
|Pooled Library||101932||Human CRISPR Deletion Library - Unassigned 1†||1||$500||Add to Cart|
|Pooled Library||101933||Human CRISPR Deletion Library - Unassigned 2†||1||$500||Add to Cart|
|Pooled Library||101934||Human CRISPR Deletion Library - Unassigned 3†||1||$500||Add to Cart|
† A Cas9 plasmid is NOT included with this item and will have to be ordered separately. Can be used with plasmids expressing Cas9 such as lentiCas9-Blast (#52962) or otherwise used with cell lines already expressing Cas9.
If you would like to receive all nine sublibraries in a single order, please email [email protected] for pricing (if you use a distributor in your country, please contact your distributor directly for pricing).
|Pooled Library||Genes Targeted||gRNAs|
Controls~5600 unique non-targeting (750 per sublibrary - partial overlap between sublibraries); 6750 unique safe-targeting (750 per sublibrary)
Each library is delivered as suspended DNA in a microcentrifuge tube on blue ice. The tube's contents will not necessarily be frozen. For best results, minimize freeze-thaws.
Terms and Licenses
These pooled libraries were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:Human CRISPR Knockout library was a gift from Michael Bassik (Addgene #)
For your References section:Genome-scale measurement of off-target activity using Cas9 toxicity in high-throughput screens. Morgens DW, Wainberg M, Boyle EA, Ursu O, Araya CL, Tsui CK, Haney MS, Hess GT, Han K, Jeng EE, Li A, Snyder MP, Greenleaf WJ, Kundaje A, Bassik MC. Nat Commun. 2017 May 5;8:15178. doi: 10.1038/ncomms15178. PubMed 28474669