Dual sgRNA CRISPRa Libraries
(Pooled Library #187249, #187250, #187251, #1000000193)
Ultra-compact, human genome-wide CRISPRa library targeting each gene with a single element encoding a dual sgRNA cassette.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Pooled Library||1000000193||Three tubes, each with a third of the library (Library 1-2, 3-4, and 5-6) in backbone pBA904
||1||$1200||Add to Cart|
|Pooled Library||187249||hCRISPRa_dual_1_2 pooled library†||1||$500||Add to Cart|
|Pooled Library||187250||hCRISPRa_dual_3_4 pooled library†||1||$500||Add to Cart|
|Pooled Library||187251||hCRISPRa_dual_5_6 pooled library†||1||$500||Add to Cart|
† A Cas9 plasmid is NOT included with this item and will have to be ordered separately. Can be used in conjunction with cell lines already expressing an activator Cas9.
Genes/gRNAs/plasmids21,503 dual sgRNA elements
Controls1,023 dual sgRNA control elements
This library is delivered as suspended DNA in a microcentrifuge tube on blue ice. The tube's contents will not necessarily be frozen. For best results, minimize freeze-thaws.
Terms and Licenses
User instructions are available on GitHub ( https://github.com/josephreplogle/CRISPRi-dual-sgRNA-screens).
These pooled libraries were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:Dual sgRNA CRISPRa libraries were a gift from Jonathan Weissman (Addgene #)
For your References section:Maximizing CRISPRi efficacy and accessibility with dual-sgRNA libraries and optimal effectors. Replogle JM, Bonnar JL, Pogson AN, Liem CR, Maier NK, Ding Y, Russell BJ, Wang X, Leng K, Guna A, Norman TM, Pak RA, Ramos DM, Ward ME, Gilbert LA, Kampmann M, Weissman JS, Jost M. bioRxiv 2022.07.13.499814. doi: 10.1101/2022.07.13.499814. bioRxiv