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MYC-CRISPR Library
(Pooled Library #173195)

  • Purpose

    This pooled lentiviral CRISPR knockout library targets E-boxes genome-wide. It was designed based on MYC-ChIP-seq data from several MYC-dependent cancer cell lines. It can be applied for identification of essential MYC binding sites in a wide range of human cells.

  • Vector Backbone

    lentiCRISPR v2 - Expresses Cas9

Ordering

Item Catalog # Description Quantity Price (USD)
Pooled Library 173195 MYC-CRISPR pooled library 1 $500 Add to Cart
Available to Academic and Nonprofits Only

Library Details

  • Species
    Human
  • Genes targeted
    24,981 E-boxes
  • gRNAs
    46,354
  • Controls
    4 sgRNAs targeting MYC from the Brunello library (positive control)
    1,000 non-targeting sgRNAs from the Brunello library (negative control)
  • Lentiviral Generation
    3rd

Library Shipping

This library is delivered as suspended DNA in a microcentrifuge tube on blue ice. The tube's contents will not necessarily be frozen. For best results, minimize freeze-thaws.

  • Volume
    20µL
  • Concentration
    100ng/µL

Depositor Comments

For enumeration of sgRNA read counts, we used the python script described in:
Genome-scale CRISPR-Cas9 knockout and transcriptional activation screening. Joung J, Konermann S, Gootenberg JS, Abudayyeh OO, Platt RJ, Brigham MD, Sanjana NE, Zhang F Nat Protoc. 2017 Apr;12(4):828-863. doi: 10.1038/nprot.2017.016. PMID 28333914

Further analysis can be performed with various algorithms dedicated for CRISPR screens, such as MAGeCK, EgdeR, DeSeq2. We used DeSeq2 as it allows analysis of genes/sequences targeted by one or multiple sgRNAs.

NOTE: Lentiviral vectors can recombine during library amplification, resulting in a ~1.5kb band (may run differently in uncut samples) containing the origin and ampicillin resistance cassette. This recombination product should not adversely affect library function as it does not contain lentiviral packaging sequences or the PuroR gene and will not be selected during screening. If a strong, recombined band is observed after amplification, we recommend starting with a larger prep (gigaprep) from the original sample or gel purifying the amplified library to remove the recombinant band and performing another transformation with the purified sample.

How to cite this pooled library ( Back to top )

These pooled libraries were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    MYC-CRISPR library was a gift from Agnieszka Dzikiewicz-Krawczyk (Addgene #173195)
  • For your References section:

    CRISPR/Cas9 screen for functional MYC binding sites reveals MYC-dependent vulnerabilities in K562 cells. Kazimierska M, Podralska M, Zurawek M, Wozniak T, Kasprzyk ME, Sura W, Losiewski W, Ziolkowska-Suchanek I, Kluiver JL, van den Berg A, Rozwadowska N, Dzikiewicz-Krawczyk A. bioRxiv 2021.08.02.454734. doi: 10.1101/2021.08.02.454734. bioRxiv 2021.08.02.454734