Mouse CRISPR Knockout Pooled Library (Gouda)
(Pooled Library #136987)
The mouse CRISPR Gouda lentiviral pooled library was designed for assays with limited cell numbers.
This library has 2 guides per gene, and is compatible for use with the Brie library (Addgene #73633).
pRDA_118 - does not express Cas9
|Item||Catalog #||Description||Quantity||Price (USD)|
|Pooled Library||136987||gRNA pooled library in pRDA_118†||1||$500||Add to Cart|
† A Cas9 plasmid is NOT included with this item and will have to be ordered separately. Can be used in conjunction with pLX_311-Cas9 (Addgene #96924) or any other plasmid(s) or cell lines expressing SpyoCas9.
Controls500 non-targeting controls, 500 one-intergenic site targeting controls
This library is delivered as suspended DNA in a microcentrifuge tube on blue ice. The tube's contents will not necessarily be frozen. For best results, minimize freeze-thaws.
NOTE: Lentiviral vectors can recombine during library amplification, resulting in an ~1kb band containing the origin and ampicillin resistance cassette. This recombination product should not adversely affect library function as it does not contain lentiviral packaging sequences or the PuroR gene and will not be selected during screening. If this 1kb band is observed after amplification, we recommend starting with a larger prep (gigaprep) from the original sample or gel purifying the amplified library to remove the recombinant band and performing another transformation with the purified sample.
These pooled libraries were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:Human Gouda CRISPR Gene Knockout pooled library was a gift from John Doench (Addgene #136987)
For your References section:Genetic Screens in Isogenic Mammalian Cell Lines Without Single Cell Cloning DeWeirdt PC, Sangree AK, Hanna RE, Sanson KR, Hegde M, Strand C, Persky NS, Doench JG Nat Commun. 2020 Feb 6;11(1):752. doi: 10.1038/s41467-020-14620-6. PubMed 32029722