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Genetic screens in isogenic mammalian cell lines without single cell cloning.
DeWeirdt PC, Sangree AK, Hanna RE, Sanson KR, Hegde M, Strand C, Persky NS, Doench JG
Nat Commun. 2020 Feb 6;11(1):752. doi: 10.1038/s41467-020-14620-6.
PubMed Article

Plasmids from Article

ID Plasmid Purpose
133456pXPR_213H1 promoter expresses customizable Saur-guide; EF1a promoter expresses SpyoCas9 and 2A site provides blasticidin resistance
133457pXPR_212U6 promoter expresses customizable Spyo-guide; EFS promoter expresses SaurCas9 and 2A site provides puromycin resistance
133458pRDA_186U6 promoter expresses customizable Spyo-guide; PGK promoter expresses blasticidin resistance and 2A site provides EGFP
133459pRDA_118U6 promoter expresses customizable Spyo-guide; EF1a promoter provides puromycin resistance. This vector is a derivative of the lentiGuide vector, with minor modifications to the tracrR
133460pRDA_103H1 promoter with two Tet operator (TetO) sites expresses customizable Spyo-guide; short EF1a promoter (EFS) expresses SaurCas9, 2A provides TetR, and 2A provides blasticidin resistance.
133461pXPR_124EF1a promoter expresses SpyoCas9 and P2A provides EGFP
136986Human sgRNA library Gattinara in pRDA_118 backboneExpresses sgRNAs targeting human genes
136987Mouse sgRNA library Gouda in pRDA_118 backboneExpresses sgRNAs targeting mouse genes

Antibodies from Article