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Genetic screens in isogenic mammalian cell lines without single cell cloning.
DeWeirdt PC, Sangree AK, Hanna RE, Sanson KR, Hegde M, Strand C, Persky NS, Doench JG
Nat Commun. 2020 Feb 6;11(1):752. doi: 10.1038/s41467-020-14620-6.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
136986Human sgRNA library Gattinara in pRDA_118 backboneExpresses sgRNAs targeting human genes
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136987Mouse sgRNA library Gouda in pRDA_118 backboneExpresses sgRNAs targeting mouse genes
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Antibodies from Article