Turner Lab Mouse messenger-RBP sgRNA Library
(Pooled Library #169082)
This retroviral CRISPR knockout library targets mouse messenger-RNA binding proteins with 10 single guide RNAs (sgRNAs) per gene. The retroviral backbone encodes both puromycin resistance and the CD90.1 selection marker.
- Does not express Cas
- Not available through Addgene
- Plasmid annotation and sequence (Genbank) (12.5 KB)
- Plasmid description: MSCV_hU6_BbsI-ccdB-BbsI_iScaffold_mPGK_puro-2A-Thy1.1 (MSCV_sgRNA_puro-Thy1.1) was generated in one round of cloning from Addgene Plasmid 27490: MIGR1. A PCR product encoding hU6_BbsI-ccdB-BbsI-iScaffold_mPGK_Puro-2A-Thy1.1 was ligated by Gibson assembly into a XhoI + SalI linearised vector.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Pooled Library||169082||Mouse messenger-RBP sgRNA Library†||1||$400||Add to Cart|
† A Cas9 plasmid is NOT included with this item and will have to be ordered separately. Can be used in conjunction with lentiCas9-Blast (Addgene #52962) or established Cas9 mouse models.
Controls500 non-targeting sgRNAs
This library is delivered as suspended DNA in a microcentrifuge tube on blue ice. The tube's contents will not necessarily be frozen. For best results, minimize freeze-thaws.
Please visit https://www.biorxiv.org/content/10.1101/2021.07.21.453193v1 (Link opens in a new window) for bioRxiv preprint.
These pooled libraries were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:Mouse messenger-RBP sgRNA Library was a gift from Martin Turner (Addgene #169082)
For your References section:A functional screen of RNA binding proteins identifies genes that promote or limit the accumulation of CD138+ plasma cells. Turner DJ, Saveliev A, Salerno F, Matheson LS, Screen M, Lawson H, Wotherspoon D, Kranc KR, Turner M. eLife 2022;11:e72313. doi: 10.7554/eLife.72313 PMID: 35451955