Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected]. Learn more

Turner Lab Mouse messenger-RBP sgRNA Library
(Pooled Library #169082)

  • Purpose

    This retroviral CRISPR knockout library targets mouse messenger-RNA binding proteins with 10 single guide RNAs (sgRNAs) per gene. The retroviral backbone encodes both puromycin resistance and the CD90.1 selection marker.

  • Vector Backbone
    • MSCV_hU6_BbsI-ccdB-BbsI_iScaffold_mPGK_puro-2A-Thy1.1
      • Does not express Cas
      • Not available through Addgene
    • Plasmid annotation and sequence (Genbank) (12.5 KB)
    • Plasmid description: MSCV_hU6_BbsI-ccdB-BbsI_iScaffold_mPGK_puro-2A-Thy1.1 (MSCV_sgRNA_puro-Thy1.1) was generated in one round of cloning from Addgene Plasmid 27490: MIGR1. A PCR product encoding hU6_BbsI-ccdB-BbsI-iScaffold_mPGK_Puro-2A-Thy1.1 was ligated by Gibson assembly into a XhoI + SalI linearised vector.


Item Catalog # Description Quantity Price (USD)
Pooled Library 169082 Mouse messenger-RBP sgRNA Library 1 $ 430 Add to Cart
Available to Academic and Nonprofits Only
  • A Cas9 plasmid is NOT included with this item and will have to be ordered separately. Can be used in conjunction with lentiCas9-Blast (Addgene #52962) or established Cas9 mouse models.

Library Details

  • Species
  • Genes targeted
  • gRNAs
  • Controls
    500 non-targeting sgRNAs

Library Shipping

This library is delivered as suspended DNA in a microcentrifuge tube on blue ice. The tube's contents will not necessarily be frozen. For best results, minimize freeze-thaws.

  • Volume
    ∼20 µL
  • Concentration
    50 ng/µL

Depositor Comments

  • Turner Lab Mouse messenger-RBP Library Overview

  • (A) Schematic representation of retrovirus sgRNA library that encodes puromycin resistance and CD90.1 (Thy1.1) selection markers, and encodes an improved sgRNA scaffold that lacks four consecutive thymine residues and has an extended duplex region. (Top) Schematic representation of vector backbone that encodes ccdB toxin gene to improve cloning efficiency. (B) Distribution of the representation of sgRNAs in library. The library is normally distributed and tightly distributed.

  • Dang, Y. et al. Optimizing sgRNA structure to improve CRISPR-Cas9 knockout efficiency. Genome Biol. (2015) doi:10.1186/s13059-015-0846-3.

Please visit (Link opens in a new window) for bioRxiv preprint.

How to cite this pooled library ( Back to top )

These pooled libraries were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Mouse messenger-RBP sgRNA Library was a gift from Martin Turner (Addgene #169082)
  • For your References section:

    A functional screen of RNA binding proteins identifies genes that promote or limit the accumulation of CD138+ plasma cells. Turner DJ, Saveliev A, Salerno F, Matheson LS, Screen M, Lawson H, Wotherspoon D, Kranc KR, Turner M. eLife 2022;11:e72313. doi: 10.7554/eLife.72313 PMID: 35451955