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Parts Human Genome-Wide Reduced Double-gRNA Library
(Pooled Library #137999)

  • Purpose

    This lentiviral CRISPR knockout library enables genome-wide screens in human cell lines at reduced scale. Through optimization of guide RNA designs and delivery of two gRNAs with each construct a five-fold reduction in size is achieved without impacting performance compared with a sgRNA library with five gRNAs per target.

  • Vector Backbone

    pKLV2-U6gRNA5(BbsI)-PGKpuro2ABFP-W - does not express Cas9


Item Catalog # Description Quantity Price (USD)
Pooled Library 137999 Human Genome-Wide Reduced Double-gRNA library 1 $ 540 Add to Cart
Available to Academic and Nonprofits Only
  • A Cas9 plasmid is NOT included with this item and will have to be ordered separately. Can be used in conjunction with pKLV2-EF1a-Cas9Bsd-W (Addgene #68343) or other plasmids or cell lines expressing Cas9.

Library Details

  • Species
  • Genes targeted
  • gRNAs
  • Controls
    398 non-targeting controls
  • Lentiviral Generation

Library Shipping

This library is delivered as suspended DNA in a microcentrifuge tube on blue ice. The tube's contents will not necessarily be frozen. For best results, minimize freeze-thaws.

  • Volume
  • Concentration

Depositor Comments

bioRxiv pre-print:
How to cite this pooled library ( Back to top )

These pooled libraries were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Human Reduced Double-gRNA Library was a gift from Leopold Parts (Addgene #137999)
  • For your References section:

    Minimized double guide RNA libraries enable scale-limited CRISPR/Cas9 screens. Peets EM, Crepaldi L, Zhou Y, Allen F, Elmentaite R, Noell G, Turner G, Iyer V, Parts L. bioRxiv BioRxiv 859652v1