Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected]. Learn more


Guigo Lab BLaER1 pgRNA Library
(Pooled Library #183825)

  • Purpose

    The BLaER1 pgRNA CRISPR library is a CRISPR-Cas9 library to target lncRNAs and protein-coding genes with a potential role in B-cell to macrophage transdifferentiation. It can be a resource to perform knockout screens in other models, such as macrophage differentiation.

  • Vector Backbone

    pDECKO_mCherry - does not express Cas9


Item Catalog # Description Quantity Price (USD)
Pooled Library 183825 BLaER1 pgRNA CRISPR library 1 $ 430 Add to Cart
Available to Academic and Nonprofits Only
  • A Cas9 plasmid is NOT included with this item and will have to be ordered separately. Can be used in conjunction with pLentiCas9-BFP (Addgene #78545) or pLentiCas9-GFP (Addgene #78546) or any other plasmid(s) or cell lines expressing Cas9.

Library Details

  • Species
  • Genes targeted
    166 lncRNAs, 874 protein-coding genes (10 pgRNA per lncRNA or protein-coding gene)
  • Controls
    4 positive controls plus pgRNAs against EGFP, mCherry and tdTomato (50 pgRNAs each); 100 negative controls (10 pgRNAs each)
  • gRNAs
  • Lentiviral Generation

Library Shipping

This library is delivered as suspended DNA in a microcentrifuge tube on blue ice. The tube's contents will not necessarily be frozen. For best results, minimize freeze-thaws.

  • Volume
    ∼25 µL
  • Concentration
    40 ng/µL

Depositor Comments

Schematic of plasmid library design and function

pgRNA Library schematic: (A) Workflow of the CRISPR screening experiment. The plasmid library is transfected into HeK293T cells to obtain a library of lentivirus. BLaER1-Cas9 cells are infected and selected with antibiotics, then induced for transdifferentiation into macrophages (3 or 6 days). Cells are labelled with antibodies against cell surface markers: CD19 (for B-lymphocytes) and Mac-1 (for macrophages). Transdifferentiation status is assessed and cell populations are isolated by Fluorescence-Activated Cell Sorting (FACS). (B) Scheme of pDECKO_mCherry plasmid and PCR amplification. H1 and U6 promoters drive the expression of the two gRNAs. In the first PCR step, staggered primers anneal to the U6 promoter and to the pDECKO backbone. In the second PCR step, primers containing the sample barcode and P5/P7 sequences for sequencing are added. (C) Position of pgRNAs targeting lncRNAs (targeting the promoter and the transcription start site) and pc-genes (targeting coding exons). (D) Library composition (number of targets of each biotype and pgRNA pairs designed per target).

Figure adapted from Arnan and Ullrich et al. 2022 BMC Genomics.

For more details on protocols and analysis, please see the publication (Arnan and Ullrich et al. 2022) or the preprint on bioRxiv (Link opens in a new window).

How to cite this pooled library ( Back to top )

These pooled libraries were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    BLaER1 pgRNA Library was a gift from Roderic Guigo (Addgene #183825)
  • For your References section:

    Paired guide RNA CRISPR-Cas9 screening for protein-coding genes and lncRNAs involved in transdifferentiation of human B-cells to macrophages. Arnan C, Ullrich S, Pulido-Quetglas C, Nurtdinov R, Esteban A, Blanco-Fernandez J, Aparicio-Prat E, Johnson R, Pérez-Lluch S, Guigó R. BMC Genomics. 2022 May 26;23(1):402. doi: 10.1186/s12864-022-08612-7; doi: 10.1186/s12864-022-08612-7. PMID: 35619054.