Human Paired-guide RNA (pgRNA) Library for Long Non-coding RNAs (lncRNAs)
(Pooled Library #89640)
- Purpose
This library is designed for genomic deletion screening of functional lncRNAs (long non-coding RNAs) using lentiviral pgRNAs (paired-guide RNAs).
- Vector Backbone
Modified scaffold-linker-U6 - this backbone does not contain Cas9.
Backbone modification includes second U6 insertion. View sequencing result here.
- Depositing Lab
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | ||
|---|---|---|---|---|---|---|
| Pooled Library | 89640 | Human pgRNA library lncRNAs (long non-coding RNAs)† | 1 | $ 430 | Add to Cart | |
† A Cas9 plasmid is NOT included with this item and will have to be ordered separately.
Library Details
-
SpeciesHuman
-
Genes targeted671 long non-coding RNAs (lncRNAs)
-
gRNAs12,472 pairs
-
Controls2000 positive; 500 negative
-
Lentiviral Generation3rd
Library Shipping
This library will be delivered as a pooled DNA library in a microcentrifuge tube on blue ice. The tube's contents will not necessarily be frozen. For best results, minimize freeze-thaw cycles.
You will receive:
-
Volume∼20µL
-
Concentration50ng/µL
Resource Information
-
Protocols
Depositor recommends using publication for library protocols.
-
Depositor Data
- Data Analysis Algorithms
- lncRNA gRNA List (1.5 MB)
-
Terms and Licenses
These pooled libraries were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the pooled libraries were described, and include Addgene in the Materials and Methods of your future publications.
Example for your Materials & Methods section:
pgRNA CRISPR-Cas9 Library for human non-coding RNAs (lncRNAs) was a gift from Wensheng Wei (Addgene # 89640).For your References section:
Genome-scale deletion screening of human long non-coding RNAs using a paired-guide RNA CRISPR-Cas9 library. Zhu S, Li W, Liu J, Chen CH, Liao Q, Xu P, Xu H, Xiao T, Cao Z, Peng J, Yuan P, Brown M, Liu XS, Wei W. Nature. Nat Biotechnol. 2016 Dec;34(12):1279-1286. doi: 10.1038/nbt.3715. Epub 2016 Oct 31. PubMed 27798563