Human Lipid Droplet and Metabolism Library
(Pooled Library #191535)

• Purpose:

  Lentiviral CRISPR library targeting 1,196 genes related to lipid droplet biology and cell metabolism. The library contains 13,920 elements (~10 guide RNAs per gene) including 2,000 negative control gRNAs. This library will be useful to researchers in the fields of lipid biology and metabolism.

• Vector Backbone:

  pMCB320 - does not express Cas9

• Depositing Labs: James Olzmann
• Publication: Roberts et al. Dev Cell. 2023 Jul 18:S1534-5807(23)00331-3. doi: 10.1016/j.devcel.2023.07.001.

Library Details

• Species: Human
• Genes targeted: 1,196
• gRNAs: 13,920
• Controls: 1,000 non-targeting and 1,000 safe guides targeting genomic locations without annotated functions
• Lentiviral Generation: 3rd

Library Shipping

This library is delivered as suspended DNA in a microcentrifuge tube on blue ice. The tube's contents will not necessarily be frozen. For best results, minimize freeze-thaws.

• Volume: ∼20 µL
• Concentration: 50 ng/µL

Resource Information

• Protocols:
  • Amplification Protocol (273 KB)
  • For library sequencing, please see the paper from Deans, et al.
• Depositor Data:
  • Human Lipid Droplet and Metabolism Library Sequences (426 MB)
  • Human Lipid Droplet and Metabolism Library Counts (529 KB)
  • Human Lipid Droplet and Metabolism Library Distribution (31 KB)
• Terms and Licenses:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)

Depositor Comments

Please visit https://www.biorxiv.org/content/10.1101/2022.08.27.505556v1 for bioRxiv preprint.