Human CRISPR Metabolic Gene Knockout Library
(Pooled Library #110066)
This lentiviral CRISPR library targets 2,981 human metabolic genes with 29,790 guide RNAs. The library contains ~10 gRNAs per gene.
lentiCRISPR v1- does express Cas9
Note: This plasmid has been discontinued, as an updated version is available. For confirmation of screen hits, Addgene encourages recipient scientists to use the updated lentiCRISPR v2, which can be requested separately.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Pooled Library||110066||gRNA pooled library||1||$400||Add to Cart|
This library is delivered as suspended DNA in a microcentrifuge tube on blue ice. The tube's contents will not necessarily be frozen. For best results, minimize freeze-thaws.
When performing sgRNA validation in a Cas9-expressing cell line, the depositing laboratory recommends using plasmid pLenti-sgRNA (Addgene #71049).
These pooled libraries were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:Human CRISPR Metabolic Gene Knockout library was a gift from David Sabatini (Addgene #110066)
For your References section:An Essential Role of the Mitochondrial Electron Transport Chain in Cell Proliferation Is to Enable Aspartate Synthesis. Birsoy K, Wang T, Chen WW, Freinkman E, Abu-Remaileh M, Sabatini DM. Cell. 2015 Jul 30;162(3):540-51. doi: 10.1016/j.cell.2015.07.016. PubMed 26232224