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Mouse Metabolism Library
(Pooled Library #163966)

  • Purpose

    This lentiviral CRISPR library targets mouse metabolic genes with ~6 guide RNAs (sgRNAs) per gene.

  • Vector Backbone

    lentiCRISPRv2 - expresses Cas9

Ordering

Item Catalog # Description Quantity Price (USD)
Pooled Library 163966 Mouse Metabolism Library 1 $400 Add to Cart
Available to Academic and Nonprofits Only

Library Details

  • Species
    Mouse
  • Genes targeted
    2,918
  • gRNAs
    18,343
  • Controls
    1,000 intergenic controls
  • Lentiviral Generation
    3rd

Library Shipping

This library is delivered as suspended DNA in a microcentrifuge tube on blue ice. The tube's contents will not necessarily be frozen. For best results, minimize freeze-thaws.

  • Volume
    ∼20µL
  • Concentration
    50ng/µL

Depositor Comments

The depositing lab has shared a GitHub repository with code for screening analysis.

How to cite this pooled library ( Back to top )

These pooled libraries were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Mouse Metabolism Library was a gift from Alec Kimmelman (Addgene #163966)
  • For your References section:

    Functional Genomics Identifies Metabolic Vulnerabilities in Pancreatic Cancer. Biancur DE, Kapner KS, Yamamoto K, Banh RS, Neggers JE, Sohn ASW, Wu W, Manguso RT, Brown A, Root DE, Aguirre AJ, Kimmelman AC. Cell Metab. 2020 Oct 28. pii: S1550-4131(20)30551-9. doi: 10.1016/j.cmet.2020.10.0186. PubMed 33152323