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Focused Ras Synthetic Lethal Human CRISPR Knockout Library
(Pooled Library #92352)

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  • Purpose

    The Sabatini/Lander CRISPR pooled library is a focused gRNA library that targets synthetic lethal candidate and control genes with oncogenic Ras.

  • Vector Backbone

    lentiCRISPR v1 - does express Cas9

    Note: This plasmid has been discontinued, as an updated version is available. For confirmation of screen hits, Addgene encourages users to use the updated lentiCRISPR v2, which can be requested separately.

Ordering

Item Catalog # Description Quantity Price (USD)
Pooled Library 92352 gRNA library in lentiCRISPRv1 1 $275 Add to Cart
This material is available to academics and nonprofits only.

Library Details

  • Species
    Human
  • Genes targeted
    132
  • gRNAs
    6,661
  • Controls
    499 intergenic
  • Lentiviral Generation
    3rd

Library Shipping

This library will be delivered as a pooled DNA library in a microcentrifuge tube on blue ice. The tube's contents will not necessarily be frozen. For best results, minimize freeze/thaw cycles.

  • Volume
    ∼10 µL
  • Concentration
    10 ng/µL

Resource Information

Depositor Comments

Graph with y axis showing Cumulative count and x axis sgRNAs ranked by decreasing abundance. The curve is slightly sloped.

Figure 1: Distribution of sgRNA counts in the initial cell population.

When performing sgRNA validation in a Cas9-expressing cell line, the depositing laboratory recommends using plasmid pLenti-sgRNA (Addgene #71049).

How to cite this pooled library (Back to top )

These pooled libraries were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the pooled libraries were described, and include Addgene in the Materials and Methods of your future publications.

  • Example for your Materials & Methods section:

    Focused Ras Synthetic Lethal Human CRISPR Knockout Library was a gift from David Sabatini and Eric Lander (Addgene # 92352)

  • For your References section:

    Gene Essentiality Profiling Reveals Gene Networks and Synthetic Lethal Interactions with Oncogenic Ras. Wang T, Yu H, Hughes NW, Liu B, Kendirli A, Klein K, Chen WW, Lander ES, Sabatini DM. Cell. 2017 Feb 1. pii: S0092-8674(17)30061-2. doi: 10.1016/j.cell.2017.01.013. PubMed 28162770 (Link opens in a new window)