Ingolia lab S cerevisiae CRISPRi v1 – barcodes 181005
(Pooled Library #161769)

• Purpose:

  This yeast CRISPR inhibition library targets the yeast genome with Tet-inducible guide RNAs linked to 25 nucleotide barcodes.

• Vector Backbone:

  pNTI661 pRPR1(TetO)-sgRNA - does not express Cas9

• Depositing Labs: Nicholas Ingolia
• Publication: McGlincy et al BMC Genomics. 2021 Mar 23;22(1):205. doi: 10.1186/s12864-021-07518-0.

Library Details

• Species: Yeast
• Genes targeted: ~6,700
• gRNAs: 44,580
• Controls: 344 no-gRNA barcodes
• Selectable Marker: LEU2

Library Shipping

This library is delivered as suspended DNA in a microcentrifuge tube on blue ice. The tube's contents will not necessarily be frozen. For best results, minimize freeze-thaws.

• Volume: ∼20 µL
• Concentration: 50 ng/µL

Resource Information

• Protocols:
  • Library Amplification Protocol (34.1 KB)
  • Library Sequencing Protocol (31.5 KB)
• Depositor Data:
  • Barcoded gRNA Library Vector Map (63.5 KB)
  • Table of gene targets, guide RNAs, and barcodes (33.9 MB)
• Terms and Licenses:
  • UBMTA

Depositor Comments

The depositing lab built a resource page to support use of this Yeast CRISPRi Library