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Ingolia lab S cerevisiae CRISPRi v1 – barcodes 181005
(Pooled Library #161769)

  • Purpose

    This yeast CRISPR inhibition library targets the yeast genome with Tet-inducible guide RNAs linked to 25 nucleotide barcodes.

  • Vector Backbone

    pNTI661 pRPR1(TetO)-sgRNA - does not express Cas9

Ordering

Item Catalog # Description Quantity Price (USD)
Pooled Library 161769 Ingolia lab yeast CRISPRi v1 - barcodes 181005 1 $400 Add to Cart
Available to Academic and Nonprofits Only
  • A Cas9 plasmid is NOT included with this item and will have to be ordered separately. Can be used in conjunction with pNTI647 dCas9-Mxi1 TetR KanMX (Addgene #139474).

Library Details

  • Species
    Yeast
  • Genes targeted
    ~6,700
  • gRNAs
    44,580
  • Controls
    344 no-gRNA barcodes
  • Selectable Marker
    LEU2

Library Shipping

This library is delivered as suspended DNA in a microcentrifuge tube on blue ice. The tube's contents will not necessarily be frozen. For best results, minimize freeze-thaws.

  • Volume
    ∼20µL
  • Concentration
    50ng/µL

Depositor Comments

The depositing lab built a resource page to support use of this Yeast CRISPRi Library

How to cite this pooled library ( Back to top )

These pooled libraries were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Ingolia lab S cerevisiae CRISPRi v1 – barcodes 181005 library was a gift from Nicholas Ingolia (Addgene #161769)
  • For your References section:

    A genome-scale CRISPR interference guide library enables comprehensive phenotypic profiling in yeast. McGlincy NJ, Meacham ZA, Swain K, Muller R, Baum R, Ingolia NT. BMC Genomics. 2021 Mar 23;22(1):205. doi: 10.1186/s12864-021-07518-0.PubMed 33757429