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A genome-scale CRISPR interference guide library enables comprehensive phenotypic profiling in yeast.
McGlincy NJ, Meacham ZA, Reynaud KK, Muller R, Baum R, Ingolia NT
BMC Genomics. 2021 Mar 23;22(1):205. doi: 10.1186/s12864-021-07518-0. PubMed Article

Plasmids from Article

ID Plasmid Purpose
139474pNTI647 dCas9-Mxi1 TetR KanMXIntegrates CRISPRi effector and tet-responsive regulator
139475pNTI661 pRPR1(TetO)-sgRNAVector for tet-regulated sgRNA expression in yeast
161769Ingolia lab S cerevisiae CRISPRi v1 – barcodes 181005Inducible CRISPRi library

Antibodies from Article