Bradley Human CRISPR Poison Exon Knockout Library
(Pooled Library #138084)

• Purpose:

  The human pgPoison library targets 3` splice sites with paired gRNAs (pgRNAs), inducing skipping of "poison" cassette exons and corresponding upstream constitutive exons.
  
  This allows direct comparison between loss of a constitutive coding exon (knockout) with loss of a poison exon.

• Vector Backbone:

  lentiGuide-Puro - does not express Cas9

• Depositing Labs: Robert Bradley
• Publication: Thomas et al Nat Genet. 2020 Jan;52(1):84-94. doi: 10.1038/s41588-019-0555-z.

Library Details

• Species: Human
• Exons targeted: 963
• Paired gRNAs: 9,508
• Controls: 1,000 non-targeting pgRNAs
• Lentiviral Generation: 3rd

Library Shipping

This library is delivered as suspended DNA in a microcentrifuge tube on blue ice. The tube's contents will not necessarily be frozen. For best results, minimize freeze-thaws.

• Volume: ∼20 µL
• Concentration: 50 ng/µL

Resource Information

• Protocols:
  • The depositor recommends following the GeCKo v2.0 Library Amplification Protocol (272 KB)
  • Paired gRNA NGS Protocol (234 KB)
• Depositor Data:
  • List of pgRNA sequences (702 KB)
  • pgRNA NGS Read Counts (1.0 MB)
• Terms and Licenses:
  • UBMTA
  • Institut Pasteur Label License for cPPT