An in-library ligation strategy and its application in CRISPR/Cas9 screening of high-order gRNA combinations.
Lu Z, Ni K, Wang Y, Zhou Y, Li Y, Yan J, Song Q, Liu M, Xu Y, Yu Z, Guo T, Ma L
Nucleic Acids Res. 2022 Jun 24;50(11):6575-6586. doi: 10.1093/nar/gkac458. PubMed Article
Lu Z, Ni K, Wang Y, Zhou Y, Li Y, Yan J, Song Q, Liu M, Xu Y, Yu Z, Guo T, Ma L
Nucleic Acids Res. 2022 Jun 24;50(11):6575-6586. doi: 10.1093/nar/gkac458. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 192363 | Immune checkpoint (library 2) Backbone | Plasmid vector for in vivo immune checkpoint screening library (for checkpoint library) |
| 192364 | T cell activation (library 1) Backbone | Plasmid vector for in vitro Jurkat activation screening (for immune library) |
| 192365 | Gly-tRNA-gRNA scaffold for library 2 | tRNA sequence provider (for checkpoint library) |
| 192366 | Pro-tRNA-gRNA scaffold for library 2 | tRNA sequence provider (for checkpoint library) |
| 192367 | Gln-tRNA-gRNA scaffold for library 1&2 | tRNA sequence provider (for checkpoint and immune library) |
| 192368 | Gly-tRNA-gRNA scaffold for library 1 | tRNA sequence provider (for immune library) |
| 192369 | Pro-tRNA-gRNA scaffold for library 1 | tRNA sequence provider (for immune library) |