sgTrack-BFP
(Plasmid
#164273)
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Purpose(Empty Backbone) Empty sgRNA expression vector with co-expression of BFP reporter
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 164273 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonelentiCRISPRV2
- Backbone size (bp) 11536
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Vector typeMammalian Expression, Lentiviral, CRISPR
- Promoter U6 and EFS
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Selectable markersPuromycin ; BFP
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Cloning Information
- Cloning method Restriction Enzyme
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
sgTrack-BFP was a gift from Charles Rudin (Addgene plasmid # 164273 ; http://n2t.net/addgene:164273 ; RRID:Addgene_164273) -
For your References section:
Concurrent Mutations in STK11 and KEAP1 Promote Ferroptosis Protection and SCD1 Dependence in Lung Cancer. Wohlhieter CA, Richards AL, Uddin F, Hulton CH, Quintanal-Villalonga A, Martin A, de Stanchina E, Bhanot U, Asher M, Shah NS, Hayatt O, Buonocore DJ, Rekhtman N, Shen R, Arbour KC, Donoghue M, Poirier JT, Sen T, Rudin CM. Cell Rep. 2020 Dec 1;33(9):108444. doi: 10.1016/j.celrep.2020.108444. 10.1016/j.celrep.2020.108444 PubMed 33264619
Map uploaded by the depositor.
