|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||26822||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 5400
Vector typeMammalian Expression
Growth in Bacteria
Copy numberHigh Copy
Insert Size (bp)903
- Cloning method Restriction Enzyme
- 5′ sequencing primer CMV Forward
- 3′ sequencing primer TK polyA reverse (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
Articles Citing this Plasmid
Note that orientation of the insert in pcDNA does not matter -- they were cloned by TA-cloning, and either orientation is fine.
Information regarding the UTR sequences and details regarding cloning can be found in the associated publication (http://www.cell.com/cms/attachment/609702/4870828/mmc1.pdf)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pcDNA3.3_eGFP was a gift from Derrick Rossi (Addgene plasmid # 26822 ; http://n2t.net/addgene:26822 ; RRID:Addgene_26822)
For your References section:Highly Efficient Reprogramming to Pluripotency and Directed Differentiation of Human Cells with Synthetic Modified mRNA. Warren L, Manos PD, Ahfeldt T, Loh YH, Li H, Lau F, Ebina W, Mandal PK, Smith ZD, Meissner A, Daley GQ, Brack AS, Collins JJ, Cowan C, Schlaeger TM, Rossi DJ. Cell Stem Cell. 2010 Sep 29. ():. 10.1016/j.stem.2010.08.012 PubMed 20888316