pLC-Flag-SENP8 sg2R-P2A-Hygro
(Plasmid #124300)

• Purpose: Lentiviral vector for expression of Flag tagged SENP8-P2A-Hygro casette from a CMV promoter. SENP8 cDNA contains silent mutations to disrupt a sgRNA binding site.
• Depositing Lab: Eva Gottwein
• Publication: Patil et al Blood Adv. 2019 Jul 23;3(14):2105-2117. doi: 10.1182/bloodadvances.2019031732.

Backbone

• Vector backbone: pLCE
• Total vector size (bp): 8208
• Vector type: Mammalian Expression, Lentiviral
• Selectable markers: Hygromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: SENP8
• Species: H. sapiens (human)
• Insert Size (bp): 633
• Mutation: several silent mutations to disrupt sgRNA targeting
• Entrez Gene: SENP8 (a.k.a. DEN1, NEDP1, PRSC2)
• Promoter: CMV
• Tag / Fusion Protein:
  • Flag (N terminal on insert)

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: -
• 3′ sequencing primer: -

Resource Information

• Supplemental Documents:
  • pLC Flag-SENP8_P2A_Hygro sgRNA D2 resistant.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry