|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||96918||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector typeMammalian Expression, Lentiviral
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Growth Strain(s)NEB Stable
- Promoter EF1a
/ Fusion Protein
- KRAB (N terminal on insert)
- Cloning method Unknown
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pLX_311-KRAB-dCas9 was a gift from John Doench & William Hahn & David Root (Addgene plasmid # 96918 ; http://n2t.net/addgene:96918 ; RRID:Addgene_96918)
For your References section:Complementary information derived from CRISPR Cas9 mediated gene deletion and suppression. Rosenbluh J, Xu H, Harrington W, Gill S, Wang X, Vazquez F, Root DE, Tsherniak A, Hahn WC. Nat Commun. 2017 May 23;8:15403. doi: 10.1038/ncomms15403. 10.1038/ncomms15403 PubMed 28534478