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pLX_311-KRAB-dCas9
(Plasmid #96918)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 96918 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    dCas9
  • Mutation
    dCas9
  • Promoter EF1a
  • Tag / Fusion Protein
    • KRAB (N terminal on insert)

Cloning Information

  • Cloning method Unknown

Resource Information

  • Supplemental Documents
  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry
  • Articles Citing this Plasmid
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLX_311-KRAB-dCas9 was a gift from John Doench & William Hahn & David Root (Addgene plasmid # 96918 ; http://n2t.net/addgene:96918 ; RRID:Addgene_96918)
  • For your References section:

    Complementary information derived from CRISPR Cas9 mediated gene deletion and suppression. Rosenbluh J, Xu H, Harrington W, Gill S, Wang X, Vazquez F, Root DE, Tsherniak A, Hahn WC. Nat Commun. 2017 May 23;8:15403. doi: 10.1038/ncomms15403. 10.1038/ncomms15403 PubMed 28534478