We narrowed to 8,881 results for: plat

1. FB026

   Plasmid

   #119711

   Purpose

   Transcriptional Unit (TU) for YFP expression, obtained by combining FB/GBparts FB007+GB0053+FB008 into pDGB3alpha1R. According to FungalBraid/GoldenBraid modular DNA assembly for ATMT

   Depositor

   Jose Marcos

   Article

   Hernanz-Koers et al Fungal Genet Biol. 2018 Jul;116:51-61. d

   Insert

   Promoter gpdA:YFP coding sequence:Terminator trpC
   

   Use

   Synthetic Biology

   Available Since

   March 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCHSU74.2-2.2

   Plasmid

   #115543

   Purpose

   Expression of a fungal biosynthetic gene cluster

   Depositor

   Ronald Davis

   Article

   Harvey et al Sci Adv. 2018 Apr 11;4(4):eaar5459. doi:

   Insert

   S_cerevisiae_ADH2-ORF1, S_bayanus_ADH2-ORF2, PCK1-ORF3, MLS1-ORF4, S_paradoxus_ADH2-ORF5, S_kudeiaveseii_ADH2-ORF6, ICL1-ORF7
   

   Expression

   Yeast

   Available Since

   March 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCHKU16.1-2.2

   Plasmid

   #115529

   Purpose

   Expression of a fungal biosynthetic gene cluster

   Depositor

   Ronald Davis

   Article

   Harvey et al Sci Adv. 2018 Apr 11;4(4):eaar5459. doi:

   Insert

   S_cerevisiae_ADH2-ORF1, S_bayanus_ADH2-ORF2, PCK1-ORF3, MLS1-ORF4, S_paradoxus_ADH2-ORF5
   

   Expression

   Yeast

   Available Since

   Oct. 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCHKU20.1-2.2

   Plasmid

   #115531

   Purpose

   Expression of a fungal biosynthetic gene cluster

   Depositor

   Ronald Davis

   Article

   Harvey et al Sci Adv. 2018 Apr 11;4(4):eaar5459. doi:

   Insert

   S_cerevisiae_ADH2-ORF1, S_bayanus_ADH2-ORF2, PCK1-ORF3, MLS1-ORF4, S_paradoxus_ADH2-ORF5, S_kudeiaveseii_ADH2-ORF6
   

   Expression

   Yeast

   Available Since

   Oct. 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCHSU68.2-2.2

   Plasmid

   #110681

   Purpose

   Expression of a fungal biosynthetic gene cluster

   Depositor

   Ronald Davis

   Article

   Harvey et al Sci Adv. 2018 Apr 11;4(4):eaar5459. doi:

   Insert

   S_cerevisiae_ADH2-ORF1, S_bayanus_ADH2-ORF2, PCK1-ORF3, MLS1-ORF4, S_paradoxus_ADH2-ORF5, S_kudeiaveseii_ADH2-ORF6
   

   Expression

   Yeast

   Available Since

   June 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCHKU27.2-2.2

   Plasmid

   #110662

   Purpose

   Expression of a fungal biosynthetic gene cluster

   Depositor

   Ronald Davis

   Article

   Harvey et al Sci Adv. 2018 Apr 11;4(4):eaar5459. doi:

   Insert

   S_cerevisiae_ADH2-ORF1, S_bayanus_ADH2-ORF2, PCK1-ORF3, MLS1-ORF4, S_paradoxus_ADH2-ORF5
   

   Expression

   Yeast

   Available Since

   June 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCHSU75.2-2.2

   Plasmid

   #110691

   Purpose

   Expression of a fungal biosynthetic gene cluster

   Depositor

   Ronald Davis

   Article

   Harvey et al Sci Adv. 2018 Apr 11;4(4):eaar5459. doi:

   Insert

   S_cerevisiae_ADH2-ORF1, S_bayanus_ADH2-ORF2, PCK1-ORF3, MLS1-ORF4, S_paradoxus_ADH2-ORF5
   

   Expression

   Yeast

   Available Since

   June 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCHKU33.2-2.2

   Plasmid

   #110667

   Purpose

   Expression of a fungal biosynthetic gene cluster

   Depositor

   Ronald Davis

   Article

   Harvey et al Sci Adv. 2018 Apr 11;4(4):eaar5459. doi:

   Insert

   S_cerevisiae_ADH2-ORF1, S_bayanus_ADH2-ORF2, PCK1-ORF3, MLS1-ORF4, S_paradoxus_ADH2-ORF5, S_kudeiaveseii_ADH2-ORF6
   

   Expression

   Yeast

   Available Since

   May 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. C21-bio-His

   Plasmid

   #53405

   Purpose

   Expresses full-length ES1 protein homolog precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

   Depositor

   Gavin Wright

   Article

   Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

   Insert

   C21 (GATD3 Human)
   

   Tags

   His tag, enzymatic biotinylation sequence, and ra…

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 27, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. HINT2-bio-His

    Plasmid

    #53406

    Purpose

    Expresses full-length Histidine triad nucleotide-binding protein 2 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

    Depositor

    Gavin Wright

    Article

    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

    Insert

    HINT2 (HINT2 Human)
    

    Tags

    His tag, enzymatic biotinylation sequence, and ra…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. CD84-bio-His

    Plasmid

    #51649

    Purpose

    Expresses full-length SLAM family member 5 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

    Depositor

    Gavin Wright

    Article

    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

    Insert

    CD84 (CD84 Human)
    

    Tags

    His tag, enzymatic biotinylation sequence, and ra…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. ACVR1-bio-His

    Plasmid

    #51623

    Purpose

    Expresses full-length Activin receptor type-1 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

    Depositor

    Gavin Wright

    Article

    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

    Insert

    ACVR1 (ACVR1 Human)
    

    Tags

    His tag, enzymatic biotinylation sequence, and ra…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pT7-PEmax for IVT

    Plasmid

    #178113

    Purpose

    Template for in vitro transcription of PEmax

    Depositor

    David Liu

    Article

    Chen et al Cell. 2021 Oct 28;184(22):5635-5652.e29.

    Insert

    PEmax
    

    Use

    Template for in vitro transcription

    Tags

    SV40 bpNLS and c-Myc NLS

    Mutation

    Detailed in manuscript

    Promoter

    T7 (inactivated)

    Available Since

    Nov. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pSL21-VEX

    Plasmid

    #158230

    Purpose

    Retroviral expression plasmid of sgRNA with violet-excited fluorescent protein (VEX)

    Depositor

    Junwei Shi

    Article

    Chen et al Cell. 2021 Mar 4;184(5):1262-1280.e22. d

    Type

    Empty backbone

    Use

    CRISPR and Retroviral

    Expression

    Mammalian

    Promoter

    U6 promoter for crRNA expression and EFS promoter…

    Available Since

    Jan. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. Anti-6xHis [N144/14R]

    Plasmid

    #177500

    Purpose

    Mammalian Expression Plasmid of anti-6xHis. Derived from hybridoma N144/14.

    Depositor

    James Trimmer

    Article

    Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

    Insert

    anti-6xHis recombinant mouse monoclonal antibody
    

    Expression

    Mammalian

    Promoter

    Dual CMV

    Available Since

    Jan. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. M6PR-bio-His

    Plasmid

    #51924

    Purpose

    Expresses full-length Cation-dependent mannose-6-phosphate receptor precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

    Depositor

    Gavin Wright

    Article

    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

    Insert

    M6PR (M6PR Human)
    

    Tags

    His tag, enzymatic biotinylation sequence, and ra…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pODN-mNG-ANLN

    Plasmid

    #183834

    Purpose

    Repair template for the N-terminal tagging of anillin with mNeonGreen in human cells using CRISPR/Cas9.

    Depositor

    Alisa Piekny

    Article

    Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

    Insert

    ANLN homology arms with mNeonGreen-linker (ANLN Human)
    

    Use

    CRISPR; Donor template

    Tags

    mNeonGreen-linker

    Expression

    Mammalian

    Mutation

    Homology arms contain point mutations to remove t…

    Available Since

    May 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3.1-GGamma9-GFP2

    Plasmid

    #140991

    Purpose

    Encodes a G gamma subunit (GNG9) containing GFP2 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation

    Depositor

    Bryan Roth

    Article

    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

    Insert

    GGamma9-GFP2 (GNGT2 Human)
    

    Tags

    GFP2 and GSAG linker

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. TGFB1-bio-His

    Plasmid

    #52185

    Purpose

    Expresses full-length Transforming growth factor beta-1 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

    Depositor

    Gavin Wright

    Article

    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

    Insert

    TGFB1 (TGFB1 Human)
    

    Tags

    His tag, enzymatic biotinylation sequence, and ra…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. qTAG-C-Solo-mStayGold-LAMP1

    Plasmid

    #227322

    Purpose

    Donor template for mStayGold insertion into the C-terminus of the LAMP1 locus. For lysosome visualization. To be co-transfected with sgRNA plasmid px330-LAMP1 (Addgene #207787)

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    LAMP1 Homology Arms flanking a mStayGold Tag (LAMP1 Human)
    

    Use

    CRISPR; Donor template

    Expression

    Mammalian

    Promoter

    Promoterless

    Available Since

    Nov. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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