We narrowed to 6,087 results for: nls

1. iPEmax-D10A

   Plasmid

   #239386

   Purpose

   For inverse prime editor using nCas9-D10A-R221K-N394K in HEK295T cells

   Depositor

   Caixia Gao

   Article

   Liang et al Nat Commun. 2025 May 31;16(1):5057. doi:

   Insert

   nCas9-D10A-R221K-N394K, M-MLV-RT
   

   Use

   CRISPR

   Tags

   BPNLS and SV40 NLS, c-myc NLS

   Expression

   Mammalian

   Mutation

   D10A, R221K, N394K in Cas9

   Promoter

   CMV

   Available since

   Aug. 4, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pHS1742

   Plasmid

   #239838

   Purpose

   OrufIscB-KRK mammalian expression

   Depositor

   Feng Zhang

   Article

   Kannan et al Nat Biotechnol. 2025 May 7. doi: 10.1038

   Insert

   OrufIscB-KRK
   

   Use

   Tags

   Nucleoplasmin NLS-3xHA and SV40 NLS

   Expression

   Mammalian

   Mutation

   Insertion of REC domain from NbaCas9-1 + E137K/E4…

   Promoter

   CMV

   Available since

   July 21, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. FUS LC 12S->G

   Plasmid

   #226597

   Purpose

   12 serines to glycines

   Depositor

   Nicolas Fawzi

   Article

   Wake et al Nat Chem Biol. 2025 Jul;21(7):1076-1088.

   Insert

   FUS LC 12S->A (FUS Human)
   

   Use

   Tags

   6His

   Expression

   Bacterial

   Mutation

   12 serines to alanine

   Promoter

   T7

   Available since

   July 1, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. FUS LC 12S->A

   Plasmid

   #226598

   Purpose

   12 serines to alanines

   Depositor

   Nicolas Fawzi

   Article

   Wake et al Nat Chem Biol. 2025 Jul;21(7):1076-1088.

   Insert

   FUS LC 12S->A (FUS Human)
   

   Use

   Tags

   6His

   Expression

   Bacterial

   Mutation

   12 serines to alanine

   Promoter

   T7

   Available since

   July 1, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. FUS LC StoG

   Plasmid

   #226607

   Purpose

   All serines to glycines

   Depositor

   Nicolas Fawzi

   Article

   Wake et al Nat Chem Biol. 2025 Jul;21(7):1076-1088.

   Insert

   FUS LC TtoS (FUS Human)
   

   Use

   Tags

   6His

   Expression

   Bacterial

   Mutation

   All threonine to serine

   Promoter

   T7

   Available since

   July 1, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. p27.pA.NGFR.mCMV.hPGK.Rab35(S22N).wpre[sequenced]

   Plasmid

   #226766

   Purpose

   Rab35(S22N)

   Depositor

   Ferdinando Pucci

   Article

   Ziglari et al Cancer Res. 2025 Mar 3;85(5):859-874. do

   Insert

   rab35-mutant S22N (RAB35 )
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 12, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. p25.pA.NGFR.mCMV.hPGK.Rab35(WT).wpre[sequenced]

   Plasmid

   #226764

   Purpose

   rab35 (WT)

   Depositor

   Ferdinando Pucci

   Article

   Ziglari et al Cancer Res. 2025 Mar 3;85(5):859-874. do

   Insert

   rab35 (RAB35 )
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 12, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. p26.pA.NGFR.mCMV.hPGK.Rab35(Q67L).wpre[sequenced]

   Plasmid

   #226765

   Purpose

   Rab35(Q67L)

   Depositor

   Ferdinando Pucci

   Article

   Ziglari et al Cancer Res. 2025 Mar 3;85(5):859-874. do

   Insert

   rab35-mutant Q67L (RAB35 )
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 12, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pPB9v2

   Plasmid

   #210029

   Purpose

   Contains cloning site for molecularly-tagged Cas9 gRNA constructs, inducible Cas9, rtTA-T2A-puro

   Depositor

   Michael Talkowski

   Article

   Nuttle et al Cell Rep Methods. 2023 Dec 5:100672. doi

   Inserts

   Cas9
   rtta-T2A-puro
   

   Use

   CRISPR

   Tags

   SV40 NLS and nucleoplasmin NLS

   Expression

   Mammalian

   Mutation

   Promoter

   EF-1α and TRE3G

   Available since

   Feb. 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pPB9

    Plasmid

    #210036

    Purpose

    Contains Cas9 gRNA cloning site, inducible Cas9, UCOE, puro-T2A-eGFP

    Depositor

    Michael Talkowski

    Article

    Nuttle et al Cell Rep Methods. 2023 Dec 5:100672. doi

    Inserts

    Cas9
    puro-T2A-eGFP
    

    Use

    CRISPR

    Tags

    SV40 NLS and nucleoplasmin NLS

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1α and TRE3G

    Available since

    Feb. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. SpCas9-NRCH-CGBE1

    Plasmid

    #198557

    Purpose

    Expresses human codon-optimized SpCas9-NRCH-CGBE1 and blasticidin resistance: EFS promoter-SpCas9-NRCH-CGBE1-NLS-FLAG-P2A-BSD

    Depositor

    Hyongbum Kim

    Article

    Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.

    Insert

    SpCas9-NRCH-CGBE1
    

    Use

    CRISPR and Lentiviral

    Tags

    BSD, FLAG, and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    EFS

    Available since

    Nov. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pHA850 - osm-10p::FynY531F

    Plasmid

    #139207

    Purpose

    Express activated Fyn kinase in osm-10 neurons

    Depositor

    Anne Hart

    Article

    Ryan et al EMBO J. 2021 Feb 1;40(3):e105001. doi: 1

    Insert

    Fyn (FYN Human)
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Y531F

    Promoter

    Available since

    Dec. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pHA852 - mec-4p::FynY531F

    Plasmid

    #139209

    Purpose

    Expresses activated Fyn kinase in mec-4 neurons

    Depositor

    Anne Hart

    Article

    Ryan et al EMBO J. 2021 Feb 1;40(3):e105001. doi: 1

    Insert

    Fyn (FYN Human)
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Y531F

    Promoter

    Available since

    Dec. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. FUS_LC_withR

    Plasmid

    #139128

    Purpose

    express FUS LC with the same number and spacing of arginines as hnRNPA2 LC

    Depositor

    Nicolas Fawzi

    Article

    Ryan et al EMBO J. 2021 Feb 1;40(3):e105001. doi: 1

    Insert

    FUS (FUS Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    add arginines

    Promoter

    Available since

    Dec. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. TOG_D1

    Plasmid

    #139112

    Purpose

    express first domain (1-250) of TOG

    Depositor

    Nicolas Fawzi

    Article

    Ryan et al EMBO J. 2021 Feb 1;40(3):e105001. doi: 1

    Insert

    TOG/CKAP5 (CKAP5 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    1-250

    Promoter

    Available since

    Dec. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. hnRNPA2_LC_nocharge_wR

    Plasmid

    #139119

    Purpose

    express hnRNPA2 LC with no charged residues with R added back

    Depositor

    Nicolas Fawzi

    Article

    Ryan et al EMBO J. 2021 Feb 1;40(3):e105001. doi: 1

    Insert

    hnRNPA2 (HNRNPA2B1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    remove all K, most D

    Promoter

    Available since

    Dec. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. hnRNPA2_LC_RtoK

    Plasmid

    #139120

    Purpose

    express hnRNPA2 LC where all R are K

    Depositor

    Nicolas Fawzi

    Article

    Ryan et al EMBO J. 2021 Feb 1;40(3):e105001. doi: 1

    Insert

    hnRNPA2 (HNRNPA2B1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    all R to K

    Promoter

    Available since

    Dec. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. hnRNPA2_LC_NtoS

    Plasmid

    #139121

    Purpose

    express hnRNPA2 with all N changed to S

    Depositor

    Nicolas Fawzi

    Article

    Ryan et al EMBO J. 2021 Feb 1;40(3):e105001. doi: 1

    Insert

    hnRNPA2 (HNRNPA2B1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    all N to S

    Promoter

    Available since

    Dec. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. hnRNPA2_LC_5E

    Plasmid

    #139122

    Purpose

    express hnRNPA2 with 5 serine changed to glutamate

    Depositor

    Nicolas Fawzi

    Article

    Ryan et al EMBO J. 2021 Feb 1;40(3):e105001. doi: 1

    Insert

    hnRNPA2 (HNRNPA2B1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    5 serine to glutamate

    Promoter

    Available since

    Dec. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. hnRNPA2_LC_12E

    Plasmid

    #139123

    Purpose

    express hnRNPA2 with all serine change to glutamate

    Depositor

    Nicolas Fawzi

    Article

    Ryan et al EMBO J. 2021 Feb 1;40(3):e105001. doi: 1

    Insert

    hnRNPA2 (HNRNPA2B1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    12 S to E

    Promoter

    Available since

    Dec. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge