A cGAL-UAS bipartite expression toolkit for Caenorhabditis elegans sensory neurons.
Nava S, Palma W, Wan X, Oh JY, Gharib S, Wang H, Revanna JS, Tan M, Zhang M, Liu J, Chen CH, Lee JS, Perry B, Sternberg PW
Proc Natl Acad Sci U S A. 2023 Dec 19;120(51):e2221680120. doi: 10.1073/pnas.2221680120. Epub 2023 Dec 14. PubMed Article
Nava S, Palma W, Wan X, Oh JY, Gharib S, Wang H, Revanna JS, Tan M, Zhang M, Liu J, Chen CH, Lee JS, Perry B, Sternberg PW
Proc Natl Acad Sci U S A. 2023 Dec 19;120(51):e2221680120. doi: 10.1073/pnas.2221680120. Epub 2023 Dec 14. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 198766 | pWP018 | Drives specific expression in the ADE, CEP, and PDE neurons |
| 198767 | pCC12 | Drives specific expression in the ADF neurons |
| 198769 | pWP169 | Drives specific expression in the ADL neurons |
| 198770 | pWP004 | Drives specific expression in the AFD neurons |
| 198771 | pHW489 | Drives specific expression in the ALA neurons |
| 198772 | pDO010 | Drives specific expression in the ALM, PLM, AVM, PVM neurons |
| 198773 | pJR032 | Stabilized step function Opsins ChR2 variant |
| 198774 | pWP052 | Drives specific expression in the ALN, PLN neurons |
| 198775 | pWP061 | Drives specific expression in the ASE neurons |
| 198776 | pWP012 | Drives specific expression in the ASE neurons |
| 198777 | pWP040 | Drives specific expression in the ASG neurons |
| 198778 | pMZ43 | Drives specific expression in the ASH neurons |
| 198779 | pMZ41 | Drives specific expression in the ASH neurons |
| 198780 | pWP017 | Drives specific expression in the ASI neurons |
| 198781 | pCC14 | Drives specific expression in the ASJ neurons |
| 198782 | pWP068 | Drives specific expression in the ASK neurons |
| 198783 | pCC20 | Drives specific expression in the ASK neurons |
| 198784 | pJL065 | Drives specific expression in the AWA neurons |
| 198785 | pWP005 | Drives specific expression in the AWB neurons |
| 198786 | pMZ9 | Drives specific expression in the AWC (ON) neurons |
| 198787 | pMZ10 | Drives specific expression in the AWC (OFF) neurons |
| 198788 | pDO068 | Drives specific expression in the BAG neurons |
| 198789 | pWP095 | Co-expression plasmid for the CEP neurons |
| 198790 | pWP097 | Co-expression plasmid for the CEP neurons |
| 198791 | pWP101 | Co-expression plasmid for the FLP, PVD neurons or the PHC neurons |
| 198792 | pWP170 | Co-expression plasmid for the FLP, PVD neurons |
| 198793 | pWP062 | Drives specific expression in the IL1 neurons |
| 198794 | pMZ3 | Drives specific expression in the IL2 neurons |
| 198795 | pWP171 | Co-expression plasmid for the OLL neurons |
| 198796 | pWP172 | Co-expression plasmid for the OLL neurons |
| 198797 | pWP034 | Drives specific expression in the OLQ neurons |
| 198798 | pWP022 | Drives specific expression in the PHA neurons |
| 198799 | pWP063 | Co-expression plasmid for the PHB neurons |
| 198800 | pWP026 | Co-expression plasmid for the PHB neurons |
| 198801 | pWP173 | Co-expression plasmid for the PHC neurons |
| 198802 | pWP066 | Drives specific expression in the URB neurons |
| 198803 | pWP003 | Drives specific expression in the URX neurons |
| 198804 | pWP085 | Drives specific expression in the URY neurons |
| 198805 | pHW449 | Direct light-gated cation channel from Chlamydomonas reinhardtii that allows neuron deploarization through brief pulses of blue light. |
| 198807 | pJL046 | In vivo neuronal inhibition through histamine-gated chloride channel. Neurons expressing HisCl1 transgene are inhibited after addition of histamine. |
| 198808 | pHW491 | In vivo blocking of synaptic signaling through Tetanus toxin mediated cleavage of synptobrevin proteins. |
| 198809 | pJL049 | In vivo calcium indicator. Presence of calcium (Ca2+) increases reporter signal intensity. |
| 198811 | pHW539 | Dominant negative form of Protein Kinase A (PKA). Lowers PKA activity in targeted neurons. |
| 198812 | pJR015 | Epidermal growth factor |
| 198813 | pJR014 | Natural light-gated anion channels. In vivo inhibition of neurons using blue light. |
| 198814 | pJR021 | Variant of yellow fluorescent protein for transient gene expression |
| 198816 | pHW580 | In vivo calcium indicator. Presence of calcium (Ca2+) increases reporter signal intensity. Based on GCaMP7, improved SNR, slow kinetics |
| 198818 | pHW581 | In vivo calcium indicator. Presence of calcium (Ca2+) increases reporter signal intensity. Based on GCaMP7, improved SNR, fast kinetics |
| 198819 | pHW582 | In vivo calcium indicator. Presence of calcium (Ca2+) increases reporter signal intensity. Based on GCaMP7, improved SNR, higher baseline fluorescence |
| 198820 | pJR022 | C. elegans optimized version of mScarlet |
| 198821 | pJR035 | For tissue specific RNA-seq |
| 198822 | pJR033 | Chloride-conducting channelrhodopsin |
| 198823 | pJR012 | Nucleolus-transport-enhanced Cre protein. Recombination-mediated gene expression knockout. |
| 198824 | pJR026 | Lysosomal associated membrane protein |
| 198825 | pJR031 | Stable step function ChR2 variant. Activated by bluelight, terminated by green light. |
| 198828 | pDO001 | Inhibition of synaptic vessel release due to disruption of synaptic vessel function by reactive oxygen species production. |
| 198829 | pJR034 | Step-Waveform Inhibitory Channelrhodopsin |
| 198831 | pHW450 | Voltage-sensitive fluorescent protein. |
| 198832 | pJR025 | Actin filament and cell cortex localization. |
| 198833 | pD002 | Inhibition of synaptic vessel release due to disruption of synaptic vessel function by reactive oxygen species production. |
| 198834 | pJR019 | Inactivation of G protein subunits through Pertussis toxin (PTX) |
| 198835 | pJR017 | In vivo blocking of synaptic signaling through Tetanus toxin mediated cleavage of synptobrevin proteins. |