We narrowed to 3,194 results for: pEGFP

1. GFP-Rab27B T23N

   Plasmid

   #89450

   Purpose

   Expression of GFP-tagged human Rab27B T23N in mammalian cells

   Depositor

   Wendy Westbroek

   Article

   Hendrix et al J Natl Cancer Inst. 2010 Jun 16;102(12):

   Insert

   human Rab27B T23N (RAB27B Human)
   

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   T23N

   Promoter

   CMV

   Available Since

   April 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCIBN-EYFP-hLaminB1

   Plasmid

   #103800

   Purpose

   BLInCR 'Localizer' construct that marks the nuclear lamina and is targeted by a PHR-tagged effector upon illumination with blue light

   Depositor

   Karsten Rippe

   Article

   Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

   Insert

   CIBN-EYFP-hLaminB1 (CIB1, LMNB1 Human, Mustard Weed)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pmCherry-Cep290

   Plasmid

   #27380

   Depositor

   Joseph Gleeson

   Article

   Valente et al Nat Genet. 2006 Jun . 38(6):623-5.

   Insert

   Cep290 (Cep290 Mouse)
   

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   please see depositor comments below

   Available Since

   June 1, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA-EGFP MASTL WT (siRNA resistant)

   Plasmid

   #191011

   Purpose

   Expresses EGFP-tagged MASTL WT with resistance to MASTL siRNA (ACGCCTTATTCTAGCAAATTA)

   Depositor

   Johanna Ivaska

   Article

   Taskinen et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

   Insert

   Microtubule-associated serine/threonine kinase like (MASTL Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Available Since

   Nov. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. Dyn1(K44A)-mRFP

   Plasmid

   #55795

   Purpose

   Dominant negative Dynamin 1 mutant tagged with mRFP

   Depositor

   Catherine Berlot

   Article

   Hynes et al J Biol Chem. 2004 Oct 15;279(42):44101-1

   Insert

   Dynamin-1(K44A)-mRFP (Dnm1 Discosoma sp., Rat)
   

   Tags

   mRFP

   Expression

   Mammalian

   Mutation

   Dynamin-1(K44A)-mRFP was produced from Dynamin-1(…

   Promoter

   CMV

   Available Since

   Sept. 1, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA-EGFP MASTL G44S (siRNA resistant)

   Plasmid

   #191012

   Purpose

   Expresses EGFP-tagged kinase-dead MASTL G44S with resistance to MASTL siRNA (ACGCCTTATTCTAGCAAATTA)

   Depositor

   Johanna Ivaska

   Article

   Taskinen et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

   Insert

   Microtubule-associated serine/threonine kinase like (MASTL Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   G44S

   Available Since

   Nov. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. p-mCherry-C1-iC++_CA

   Plasmid

   #98168

   Purpose

   slow cycling (open for minutes) step-function artificial anion conducting channelrhodopsin (aACR). High light sensitivity. Activation with cyan light, inactivation max with 590 nm (accelarates closure to ms). Codon optimized for mammalian expression.

   Depositor

   Peter Hegemann

   Article

   Wietek et al Sci Rep. 2017 Nov 2;7(1):14957. doi: 10.

   Insert

   Synthetic construct iC++_C128A gene
   

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   T59S, E83N, E90Q, E101S, V117R, E123S, C128A, V24…

   Promoter

   CMV (+enhancer)

   Available Since

   July 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. EGFP-INPP5E-CAAX

   Plasmid

   #202746

   Purpose

   express plasma membrane targeted fluorescent protein-tagged enzyme

   Depositor

   Gerry Hammond

   Article

   Wills et al J Cell Sci. 2023 Aug 3:jcs.261494. doi:

   Insert

   EGFP:INPP5E(214-644):HRAS(172-189) (HRAS, INPP5E Human)
   

   Expression

   Mammalian

   Available Since

   July 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pmiRFP2-Actin-C-18

   Plasmid

   #178965

   Purpose

   Expresses the fusion protein of miRFP2-Actin-C in mammalian cells

   Depositor

   Kiryl Piatkevich

   Article

   Babakhanova et al Protein Sci. 2022 Mar;31(3):728-751. doi

   Insert

   miRFP2-actin
   

   Tags

   actin

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. Stx5S-GFP

    Plasmid

    #209847

    Purpose

    Expresses Stx5S-GFP in mammalian cells

    Depositor

    Geert van den Bogaart

    Article

    Linders et al Nat Commun. 2021 Oct 28;12(1):6227. doi:

    Insert

    STX5 (STX5 Human)
    

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    Lacks amino acids 1-54, encodes short isoform of …

    Promoter

    CMV

    Available Since

    Nov. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pGFP-mHP1gamma

    Plasmid

    #181902

    Purpose

    Fluorescently tagged HP1gamma

    Depositor

    Karsten Rippe

    Article

    Erdel et al Mol Cell. 2020 Apr 16;78(2):236-249.e7.

    Insert

    mHP1gamma (Cbx3 Mouse)
    

    Tags

    GFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. alpha actinin delta ABD-GFP

    Plasmid

    #66935

    Purpose

    Mammalian expression of alpha actinin with actin binding domain (amino acids 30–253) deleted

    Depositor

    Pere Roca-Cusachs, Michael Sheetz

    Article

    Roca-Cusachs et al Proc Natl Acad Sci U S A. 2013 Apr 9;110

    Insert

    Alpha-actinin 1 (ACTN1 Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    actin binding domain (amino acids 30–253) is dele…

    Promoter

    CMV

    Available Since

    June 30, 2015

    Availability

    Academic Institutions and Nonprofits only

13. pCIBN-hTRF1-tagRFP-T

    Plasmid

    #103811

    Purpose

    BLInCR 'Localizer' construct that marks telomeres and is targeted by a PHR-tagged effector upon illumination with blue light; can be visualized without triggering PHR recruitment

    Depositor

    Karsten Rippe

    Article

    Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

    Insert

    CIBN-hTRF1-tagRFP-T (CIB1, TERF1 Human, Mustard Weed)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCIBN-tagBFP-hLaminB1

    Plasmid

    #103799

    Purpose

    BLInCR 'Localizer' construct that marks the nuclear lamina and is targeted by a PHR-tagged effector upon illumination with blue light

    Depositor

    Karsten Rippe

    Article

    Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

    Insert

    CIBN-tagBFP-hLaminB1 (CIB1, LMNB1 Human, Mustard Weed)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. EGFP-IpgD(C439S)

    Plasmid

    #183667

    Purpose

    N-terminally tagged Shigella flexneri IpgD (catalytically inactive) for mammalian expression

    Depositor

    Sergio Grinstein

    Article

    Walpole et al Nat Cell Biol. 2022 Apr 28. pii: 10.1038

    Insert

    IpgD
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    C439S

    Promoter

    CMV

    Available Since

    May 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. p-mCherry-C1-Phobos_CA

    Plasmid

    #98169

    Purpose

    slow cycling (open for minutes) step-function artificial anion conducting channelrhodopsin (aACR). High light sensitivity. Activation with blue light, inactivation max with 580 nm (accelarates closure to ms). Codon optimized for mammalian expression.

    Depositor

    Peter Hegemann

    Article

    Wietek et al Sci Rep. 2017 Nov 2;7(1):14957. doi: 10.

    Insert

    Synthetic construct Phobos_C128A gene
    

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    T59S, E83N, E90Q, E101S, V117R, E123S, C128A, T15…

    Promoter

    CMV (+enhancer)

    Available Since

    July 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCIBN-EYFP-hTRF1

    Plasmid

    #103802

    Purpose

    BLInCR 'Localizer' construct that marks the telomeres and is targeted by a PHR-tagged effector upon illumination with blue light

    Depositor

    Karsten Rippe

    Article

    Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

    Insert

    CIBN-EYFP-hTRF1 (CIB1, TERF1 Human, Mustard Weed)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. GFP-Rab27B GG

    Plasmid

    #89451

    Purpose

    Expression of GFP-tagged human Rab27B GG in mammalian cells

    Depositor

    Wendy Westbroek

    Article

    Hendrix et al J Natl Cancer Inst. 2010 Jun 16;102(12):

    Insert

    human Rab27B GG (RAB27B Human)
    

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    GG (geranyl geranyl site is mutated)

    Promoter

    CMV

    Available Since

    April 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. p-mCherry-C1-Aurora_CA

    Plasmid

    #98170

    Purpose

    slow cycling (open for minutes) step-function artificial anion conducting channelrhodopsin (aACR). High light sensitivity. Activation with green light, inactivation max with 625 nm (accelarates closure to ms). Not recommended for in vivo use. Codon optimized for mammalian expression.

    Depositor

    Peter Hegemann

    Article

    Wietek et al Sci Rep. 2017 Nov 2;7(1):14957. doi: 10.

    Insert

    Synthetic construct Aurora_C128A gene
    

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    V59S, E83N, E90Q, E101S, V117R, E123S, C128A, P24…

    Promoter

    CMV (+enhancer)

    Available Since

    July 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pSN879

    Plasmid

    #190693

    Purpose

    Knockdown mouse KIF5A, KIF5B and KIF5C simultaneously. mScarlet::miRNA(KIF5C)::miRNA(KIF5B)::miRNA(KIF5A).

    Depositor

    Shinsuke Niwa

    Article

    Niwa et al Cytoskeleton (Hoboken). 2023 Apr 10. doi

    Insert

    mScarlet::miRNA(KIF5C)::miRNA(KIF5B)::miRNA(KIF5A) (Kif5c, Kif5b, Kif5a Synthetic)
    

    Use

    RNAi

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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