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Crystal structure of the heterodimeric CLOCK:BMAL1 transcriptional activator complex.
Huang N, Chelliah Y, Shan Y, Taylor CA, Yoo SH, Partch C, Green CB, Zhang H, Takahashi JS
Science. 2012 Jul 13;337(6091):189-94. doi: 10.1126/science.1222804. Epub 2012 May 31.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
47334pCMV10/3xflag-Clockfull length clock in pCMV10 with 3x flag at the N-terminus
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47335pfastbac/mBmal62-447mBmal fragment cloned into pfastbac and crystallized
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47336pfastbacHTb/mClock26-384mClock fragment cloned into pfastbacHTb vector with Hexa His tag
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47337pCMV10/3xflag-Clock L57EMutation used for co-IPs
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47338pCMV10/3xflag-Clock L74EMutation used for co-IPs
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47339pCMV10/3xflag-Clock L113EMutation used for co-IPs
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47340pCMV10/3xflag-Clock F122DMutation used for co-IPs
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47341pCMV10/3xflag-Clock W284AMutation used for co-IPs
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47342pCMV10/3xflag-Clock V315RMutation used for co-IPs
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47343pCMV10/3xflag-Clock L57E/L113E/W284Atriple mutation used for co-IP
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47344pCDNA3-HA/Bmal L95EPcHA-Bmal backbone used for the above mutation. Ref.Genes & Dev 17:1921-1932 2003
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47345pCDNA3-HA/Bmal L115EPcHA-Bmal backbone used for the above mutation. Ref.Genes & Dev 17:1921-1932 2003
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47346pCDNA3-HA/Bmal L150EPcHA-Bmal backbone used for the above mutation. Ref.Genes & Dev 17:1921-1932 2003
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47347pCDNA3-HA/Bmal I317DPcHA-Bmal backbone used for the above mutation. Ref.Genes & Dev 17:1921-1932 2003
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47348pCDNA3-HA/BmalF423RPcHA-Bmal backbone used for the above mutation. Ref.Genes & Dev 17:1921-1932 2003
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47349pCDNA3-HA/Bmal W427APcHA-Bmal backbone used for the above mutation. Ref.Genes & Dev 17:1921-1932 2003
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47350pCDNA3-HA/Bmal V435RPcHA-Bmal backbone used for the above mutation. Ref.Genes & Dev 17:1921-1932 2003
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47351pCDNA3-HA/Bmal F423R/V435R/W427RPcHA-Bmal backbone used for the above mutation. Ref.Genes & Dev 17:1921-1932 2003
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47352pCDNA3-HA/Bmal L95E/L150E/W427APcHA-Bmal backbone used for the above mutation. Ref.Genes & Dev 17:1921-1932 2003
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47353HLH Clock Pas 1-401 VenNClock fragment cloned and tagged with VenN
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47354HLH Clock Pas 1-401 VenN L57EClock fragment mutatation tagged with VenN
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47355HLH Clock Pas 1-401 VenN L74EClock fragment mutatation tagged with VenN used for BiFC
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47356HLH Clock Pas 1-401 VenN L113EClock fragment mutatation tagged with VenN used for BiFC
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47357HLH Clock Pas 1-401 VenN F122DClock fragment mutatation tagged with VenN used for BiFC
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47358HLH Clock Pas 1-401 VenN W284AClock fragment mutatation tagged with VenN used for BiFC
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47359HLH Clock Pas 1-401 VenN V315RClock fragment mutatation tagged with VenN used for BiFC
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47360HLH Clock Pas 1-401 VenN L57E/L113E/W284AClock fragment mutatation tagged with VenN used for BiFC
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47364HLH Bmal Pas 1-465-VenCBmal fragment cloned with Venus tag and used for BiFC
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47365HLH Bmal Pas 1-465-VenC L95EBmal fragment cloned with Venus tag used for mutation
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47366HLH Bmal Pas 1-465-VenC L150EBmal fragment cloned with Venus tag used for mutation
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47367HLH Bmal Pas 1-465-VenC I317DBmal fragment cloned with Venus tag used for mutation
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47368HLH Bmal Pas 1-465-VenC F423RBmal fragment cloned with Venus tag used for mutation
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47369HLH Bmal Pas 1-465-VenC W427ABmal fragment cloned with Venus tag used for mutation
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47370HLH Bmal Pas 1-465-VenC V435RBmal fragment cloned with Venus tag used for mutation
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47371HLH Bmal Pas 1-465-VenC F423R/V435RBmal fragment cloned with Venus tag used for mutation
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47372HLH Bmal Pas 1-465-VenC L95E/L150E/W427ABmal fragment cloned with Venus tag used for mutation
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47374LV-CMV-Cer-FL- Bmal L95EBmal full length with cer tag used for mutation
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47376LV-H2B-mRFP1This was used as a nuclear marker
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47377HLH Bmal Pas 1-465-VenC L115EBmal fragment cloned with Venus tag used for mutation
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Antibodies from Article