We narrowed to 5,068 results for: codon optimized

1. pcDNA5-FRT-TO-Nsp2

   Plasmid

   #157682

   Purpose

   mammalian expression of untagged SARS-CoV-2 Nsp2 under control of a tetracycline-inducible promoter

   Depositor

   David Tollervey

   Article

   Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

   Insert

   Nsp2 (ORF1ab SARS-CoV-2)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   codon optimized

   Promoter

   Available since

   Aug. 4, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. pYPQ290-D570A

   Plasmid

   #129673

   Purpose

   Gateway entry clone for CRISPR-Cas12b systems

   Depositor

   Yiping Qi

   Article

   Ming et al Nat Plants. 2020 Mar;6(3):202-208. doi:

   Insert

   AacCas12b
   

   Use

   CRISPR; Gateway compatible aaccas12b-d570a entry …

   Tags

   Expression

   Plant

   Mutation

   D570A; AacCas12b is rice codon optimized

   Promoter

   Available since

   March 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pYPQ292-D570A-SRDX

   Plasmid

   #129684

   Purpose

   Gateway entry clone for CRISPR-Cas12b systems

   Depositor

   Yiping Qi

   Article

   Ming et al Nat Plants. 2020 Mar;6(3):202-208. doi:

   Insert

   AaCas12b-SRDX
   

   Use

   CRISPR; Gateway compatible aacas12b entry clone

   Tags

   Expression

   Plant

   Mutation

   D570A; AaCas12b is rice codon optimized

   Promoter

   Available since

   March 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDONR221_SLC39A5

   Plasmid

   #132057

   Purpose

   Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

   Depositor

   RESOLUTE Consortium, Giulio Superti-Furga

   Article

   Polesel et al Biometals. 2022 Dec 1. doi: 10.1007/s105

   Insert

   SLC39A5 (SLC39A5 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 8, 2019

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. hSRAP.HMG6.wt.Dlink.a1-236

   Plasmid

   #49813

   Purpose

   expression vector for MBP-SRAP(1-236) fusion

   Depositor

   Thomas Cech

   Article

   McKay et al J Mol Biol. 2014 Apr 17;426(8):1766-85.

   Insert

   steroid receptor RNA activator protein (SRA1 Human)
   

   Use

   Tags

   E. coli Maltose Binding Protein

   Expression

   Bacterial

   Mutation

   wildtype codon-optimized for E. coli expression

   Promoter

   T7

   Available since

   Jan. 16, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLV-Hygro-TRE>hHES3:T2A:EGFP

   Plasmid

   #236630

   Purpose

   Hygromycin selected lentiviral construct with tetracyclin-inducible human HES3

   Depositor

   Genevieve Kendall

   Article

   Kent et al Sci Rep. 2024 Sep 5;14(1):20773. doi: 10

   Inserts

   HES3 (HES3 Human)
   EGFP
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Codon optimized based on a variant of wildtype GF…

   Promoter

   TRE

   Available since

   June 12, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pTMiniTrex-mCherry-Shark3

   Plasmid

   #233145

   Purpose

   Serves as a template plasmid for amplifying Shark3 (Theo-OFF) hammerhead aptazyme-3xTy-tagging cassette

   Depositor

   Ronald Etheridge

   Article

   Wiedeman et al Proc Natl Acad Sci U S A. 2025 Feb 25;12

   Inserts

   mCherry
   Shark3
   

   Use

   Prokaryotic expression

   Tags

   3x Ty tag

   Expression

   Mutation

   Codon optimized for Trypanosoma cruzi and changed…

   Promoter

   Available since

   March 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCS2-phiC31o (JDW 453)

   Plasmid

   #229842

   Purpose

   A CMV-driven Phi31 recombinase.

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

   Insert

   phiC31 codon optimized, PhiC31o
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 13, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pEND-225_Cacnes_mKO2

   Plasmid

   #225612

   Purpose

   Replicative vector for orange fluorescent reporter protein mKO2 strong constitutive recombinant expression in C. acnes. pBRESP36A with P(BBa_J23119)+RBS_1+mKO2

   Depositor

   Marc Güell

   Article

   Nevot et al Cell Syst. 2025 Feb 19;16(2):101169. doi

   Insert

   mKO2
   

   Use

   Synthetic Biology

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Codon optimized for Cutibacterium acnes

   Promoter

   Available since

   Feb. 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. SpCas9-NRCH-CGBE1

    Plasmid

    #198557

    Purpose

    Expresses human codon-optimized SpCas9-NRCH-CGBE1 and blasticidin resistance: EFS promoter-SpCas9-NRCH-CGBE1-NLS-FLAG-P2A-BSD

    Depositor

    Hyongbum Kim

    Article

    Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.

    Insert

    SpCas9-NRCH-CGBE1
    

    Use

    CRISPR and Lentiviral

    Tags

    BSD, FLAG, and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    EFS

    Available since

    Nov. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pGGI_Myr-YFP-MiniTurboID

    Plasmid

    #222432

    Purpose

    Potential plasma membrane control.

    Depositor

    Dominique Bergmann

    Article

    Wallner et al Dev Cell. 2024 May 6;59(9):1096-1109.e5.

    Insert

    MiniTurboID (BirA mutant)
    

    Use

    Golden gate / green gate compatible cloning vector

    Tags

    Linker, Myr, and YFP

    Expression

    Mutation

    aa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …

    Promoter

    Available since

    July 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET151-GyrA_F164R238

    Plasmid

    #216230

    Purpose

    S. aureus USA300 GyrA mutant F164 R238 residues codon-optimized for E. coli

    Depositor

    Eric Brown

    Article

    French et al Cell Rep. 2024 Apr 4;43(4):114053. doi:

    Insert

    DNA gyrase
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Changed arginine 238 to serine, phenylalanine 168…

    Promoter

    T7

    Available since

    April 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET151-GyrB_A467

    Plasmid

    #216234

    Purpose

    S. aureus USA300 GyrB mutant A467 residue codon-optimized for E. coli

    Depositor

    Eric Brown

    Article

    French et al Cell Rep. 2024 Apr 4;43(4):114053. doi:

    Insert

    DNA gyrase
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Changed alanine 467 to valine

    Promoter

    T7

    Available since

    April 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET151-GyrB_T522

    Plasmid

    #216235

    Purpose

    S. aureus USA300 GyrB mutant T522 residue codon-optimized for E. coli

    Depositor

    Eric Brown

    Article

    French et al Cell Rep. 2024 Apr 4;43(4):114053. doi:

    Insert

    DNA gyrase
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Changed threonine 522 to isoleucine

    Promoter

    T7

    Available since

    April 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. P15-pZE-SUMO-PiTTA

    Plasmid

    #204630

    Purpose

    ColE1 ori, KanR, TetR, Tet promoter with a codon optimized piTTA gene bearing an N-terminal hexahistidine tag followed by a SUMO tag and a TEV protease cleavage site.

    Depositor

    Aditya Kunjapur

    Article

    Jones et al Commun Biol. 2023 Sep 11;6(1):929. doi:

    Insert

    His-SUMO-TEV-BuTTA
    

    Use

    Synthetic Biology

    Tags

    His Tag; SUMO Tag; TEV Protease Site

    Expression

    Bacterial

    Mutation

    Promoter

    PLtetO-1 promoter

    Available since

    Nov. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. P17-pZE-SUMO-CsTTA

    Plasmid

    #204631

    Purpose

    ColE1 ori, KanR, TetR, Tet promoter with a codon optimized csTTA gene bearing an N-terminal hexahistidine tag followed by a SUMO tag and a TEV protease cleavage site.

    Depositor

    Aditya Kunjapur

    Article

    Jones et al Commun Biol. 2023 Sep 11;6(1):929. doi:

    Insert

    His-SUMO-TEV-CsTTA
    

    Use

    Synthetic Biology

    Tags

    His Tag; SUMO Tag; TEV Protease Site

    Expression

    Bacterial

    Mutation

    Promoter

    PLtetO-1 promoter

    Available since

    Nov. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. P18-pZE-SUMO-BuTTA

    Plasmid

    #204632

    Purpose

    ColE1 ori, KanR, TetR, Tet promoter with a codon optimized buTTA gene bearing an N-terminal hexahistidine tag followed by a SUMO tag and a TEV protease cleavage site.

    Depositor

    Aditya Kunjapur

    Article

    Jones et al Commun Biol. 2023 Sep 11;6(1):929. doi:

    Insert

    His-SUMO-TEV-BuTTA
    

    Use

    Synthetic Biology

    Tags

    His Tag; SUMO Tag; TEV Protease Site

    Expression

    Bacterial

    Mutation

    Promoter

    PLtetO-1 promoter

    Available since

    Nov. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. P24-pZE-SUMO-KaTTA

    Plasmid

    #204633

    Purpose

    ColE1 ori, KanR, TetR, Tet promoter with a codon optimized kaTTA gene bearing an N-terminal hexahistidine tag followed by a SUMO tag and a TEV protease cleavage site.

    Depositor

    Aditya Kunjapur

    Article

    Jones et al Commun Biol. 2023 Sep 11;6(1):929. doi:

    Insert

    His-SUMO-TEV-KaTTA
    

    Use

    Synthetic Biology

    Tags

    His Tag; SUMO Tag; TEV Protease Site

    Expression

    Bacterial

    Mutation

    Promoter

    PLtetO-1 promoter

    Available since

    Nov. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. (B) NAT marker (R) (SBE173)

    Plasmid

    #195014

    Purpose

    nourseothricin (NAT) resistance genes codon-optimized for R. toruloides and expressed under pXYL and tNOS

    Depositor

    Petri-Jaan Lahtvee

    Article

    Bonturi et al Metab Eng Commun. 2022 May 23;15:e00200.

    Insert

    pXYL+NAT+tNOS
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pXYL

    Available since

    Nov. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. p202-Syt1-C2A-wt

    Plasmid

    #201553

    Purpose

    Expresses wild-type Octopus synaptotagmin-1 C2A domain in E. coli

    Depositor

    Joshua Rosenthal

    Article

    Birk et al Cell. 2023 Jun 8;186(12):2544-2555.e13.

    Insert

    Synaptotagmin-1 C2A domain (partial)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Codon-optimized for E. coli

    Promoter

    RBS

    Available since

    Nov. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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