159,333 results

1. pWM_12x601_25bpLinker

   Plasmid

   #157788

   Purpose

   EcoRV digestion produces 12x601 chromatin assembly construct with 25 bp linker lengths in addition to carrier DNA that prevents overassembly of nucleosomes

   Depositor

   Michael Rosen

   Article

   Gibson et al Cell. 2019 Oct 3;179(2):470-484.e21. doi

   Insert

   12x601_25bp linker
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAGD335

   Plasmid

   #219499

   Purpose

   pAGD248 derivative with the retron Eco1 msr, sfGFP flanked by 2 BsaI sites , and the rest of the retron recombineering operon (Eco1 RT, CspRect, MutL-E32K) downstream of the pBad promoter

   Depositor

   Seth Shipman

   Article

   Gonzalez-Delgado et al bioRxiv [Preprint]. 2023 Jul 17:2023.07.

   Insert

   Eco1 msr, sfGFP, Eco1 RT, CspRecT, MutLE32K
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   pBAD

   Available since

   June 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. ML1Nwt_in_pEGFP-C1

   Plasmid

   #67797

   Purpose

   Mammalian expression vector driving Mouse ML1Nwt (2xML1N domain wildtype version) fused to EGFP

   Depositor

   Rob Parton

   Article

   Ariotti et al Dev Cell. 2015 Nov 23;35(4):513-25. doi:

   Insert

   ML1N*2-EGFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 15, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET-Sac-Abeta(M1-40)

   Plasmid

   #71876

   Purpose

   Expresses the amyloid-beta protein (40 aa), containing an exogenous methionine (Abeta(M1-40)), when expressed in BL21* DE3 p LysS E. coli cells

   Depositor

   Dominic Walsh

   Article

   Walsh et al FEBS J. 2009 Mar;276(5):1266-81. doi: 10

   Insert

   Abeta(M1-40) (APP Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Jan. 19, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCMV-OptoSTIM1

   Plasmid

   #70159

   Purpose

   Mammalian expression plasmid of OptoSTIM1 (optogenetically-activated STIM1 protein)

   Depositor

   Won Do Heo

   Article

   Kyung et al Nat Biotechnol. 2015 Sep 14. doi: 10.103

   Insert

   OptoSTIM1 (STIM1 Human, Mustard Weed)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 2, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCMV-MbPylRS(DiZPK)

   Plasmid

   #91706

   Purpose

   express Methanosarcina barkeri pyrrolysyl-tRNA synthetase/tRNA pair in mammalian cells to incorporate the photocrosslinkers DiZPK, DiZSeK, or DiZHSeC into amber codon site on a protein of interest

   Depositor

   P. Chen

   Article

   Lin et al Angew Chem Int Ed Engl. 2013 Dec 23;52(5

   Inserts

   pylS
   pylT
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   T810G, C813T, C820G, T821C, T937A, A1046T, G1257A

   Promoter

   Available since

   July 20, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCyCBE

   Plasmid

   #226505

   Purpose

   A cytidine deaminase-mediated base-editing plasmid in cyanobacteria

   Depositor

   Weizhong Chen

   Article

   Li et al Commun Biol. 2024 Aug 14;7(1):994. doi:

   Inserts

   APOBEC1-Cas9D10A
   sgRNA
   sacB gene
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3.1 PRDM16

   Plasmid

   #15503

   Depositor

   Bruce Spiegelman

   Article

   Seale et al Cell Metab. 2007 Jul . 6(1):38-54.

   Insert

   PRDM16 (Prdm16 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 14, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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9. pPB-EF1A-mSTING

   Plasmid

   #229739

   Purpose

   Expresses mouse STING in mammalian cells. Stable expression with piggybac transposase.

   Depositor

   Shawn Ferguson

   Article

   Bentley-DeSousa et al J Cell Biol. 2025 Feb 3;224(2):e20231015

   Insert

   STING (Sting1 Mouse)
   

   Use

   Tags

   None

   Expression

   Mammalian

   Mutation

   Promoter

   EF1A

   Available since

   Feb. 24, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCMV-PE7-SB2

    Plasmid

    #228868

    Purpose

    PE7 connected to MLH1-SB and MLH1-SB-NLS via 2A sequence

    Depositor

    Sangsu Bae

    Insert

    PE7-2A-MLH1-SB-NLS-2A-MLH1-SB
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. TFORF0756

    Plasmid

    #141685

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    JUND (JUND Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pBA-mCherry-EGFP-PIM

    Plasmid

    #111758

    Purpose

    Low level expression of mCherry-EGFP-PIM. Can be used for inducible aggregate formation upon AP20187 addition

    Depositor

    Lukas Kapitein

    Article

    Janssen et al Nat Commun. 2018 Oct 12;9(1):4245. doi:

    Insert

    2x FKBP homo-mCherry-EGFP-2x FKBP homo-2x FKBP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Val24Glu, Tyr80Cys, Ala95Thr mutations in second …

    Promoter

    chicken beta-actin

    Available since

    Oct. 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET21a-Streptavidin-Dead

    Plasmid

    #20859

    Purpose

    Dead subunit for creating monovalent streptavidin with a single femtomolar biotin binding site. Use in combination with Streptavidin-Alive (construct 20860).

    Depositor

    Alice Ting

    Article

    Howarth et al Nat Methods. 2006 Apr . 3(4):267-73.

    Insert

    Streptavidin-Dead
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Triple mutant of wt Streptavidin N23A,S27D,S45A

    Promoter

    Available since

    April 21, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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14. pHCMV_FLUAV_HA

    Plasmid

    #207273

    Purpose

    Mammalian expression of Influenza A virus HA protein

    Depositor

    Feng Zhang

    Article

    Strebinger et al Nat Commun. 2023 Aug 23;14(1):5141. doi:

    Insert

    FLUAV_HA (HA Influenza A virus)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    mammalian codon optimization

    Promoter

    CMV promoter

    Available since

    Nov. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. RG6

    Plasmid

    #80167

    Purpose

    fluorescent protein splicing reporter

    Depositor

    Thomas Cooper

    Article

    Orengo et al Nucleic Acids Res. 2006;34(22):e148. Epu

    Insert

    dsRed and GFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pAAV-HDR-mEGFP-Actin

    Plasmid

    #119870

    Purpose

    AAV vector including gRNA expression cassette and mEGFP knock-in donor targeting mouse Beta Actin

    Depositor

    Ryohei Yasuda

    Article

    Nishiyama et al Neuron. 2017 Oct 18. pii: S0896-6273(17)

    Insert

    beta Actin (Actb Mouse)
    

    Use

    AAV, CRISPR, and Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV-N-RAM-d2tTA-TRE-mKate2

    Plasmid

    #140275

    Purpose

    N-RAM, the Npas4-dependent reporter

    Depositor

    Yingxi Lin

    Article

    Sun et al Cell. 2020 Apr 16;181(2):410-423.e17. do

    Insert

    mKate2
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    TRE

    Available since

    July 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAKTaq

    Plasmid

    #25712

    Purpose

    Bacterial expression of Taq polymerase

    Depositor

    David Engelke

    Article

    Engelke et al Anal Biochem. 1990 Dec . 191(2):396-400.

    Insert

    Taq polymerase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 24, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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19. cyto-iATPSnFR1.0

    Plasmid

    #102550

    Purpose

    cytoplasmic ATP sensor for mammalian cells

    Depositor

    Baljit Khakh

    Article

    Lobas et al Nat Commun. 2019 Feb 12;10(1):711. doi:

    Insert

    iATPSnFR1.0
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pRSETa mEos2

    Plasmid

    #20341

    Purpose

    bacterial expression of an EosFP variant, mEos2 (a photoconvertible fluorescent protein)

    Depositor

    Loren Looger

    Article

    McKinney et al Nat Methods. 2009 Feb . 6(2):131-3.

    Insert

    mEos-2
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 16, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge