|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||16351||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
Vector backbonepGEM-T Easy
- Backbone size w/o insert (bp) 3000
Vector typeBacterial Expression
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert nameHomo sapiens Nanog homeobox
SpeciesH. sapiens (human)
Insert Size (bp)900
- Cloning method Restriction Enzyme
- 5′ sequencing primer M13 Reverse
- 3′ sequencing primer T7 (Common Sequencing Primers)
This plasmid contains the Nanog sequence that was cloned into the lentivirus plasmid and used in cell reprogramming.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pGEM-Nanog was a gift from James Thomson (Addgene plasmid # 16351 ; http://n2t.net/addgene:16351 ; RRID:Addgene_16351)
For your References section:Induced Pluripotent Stem Cell Lines Derived from Human Somatic Cells. Yu J, Vodyanik MA, Smuga-Otto K, Antosiewicz-Bourget J, Frane JL, Tian S, Nie J, Jonsdottir GA, Ruotti V, Stewart R, Slukvin II, Thomson JA. Science. 2007 Nov 20. ():. 10.1126/science.1151526 PubMed 18029452