161,709 results

1. pMSCV_IRES_GFP_CD16a

   Plasmid

   #196189

   Purpose

   Retroviral expression plasmid for generating stable FCGR3A (CD16a)-expressing cell lines.

   Depositor

   Ana Ruiz-Saenz

   Article

   Vincken et al STAR Protoc. 2023 Apr 17;4(2):102224. do

   Insert

   CD16a (FCGR3A Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   changed Phenylalanine 158 to Valine

   Promoter

   LTR

   Available since

   Sept. 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pHIV-Luc-ZsGreen

   Plasmid

   #39196

   Depositor

   Bryan Welm

   Inserts

   Firefly Luciferase Luc2P
   ZsGreen
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1-alpha and EF1-alpha

   Available since

   Oct. 30, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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3. pIVT-R2Tg-WT

   Plasmid

   #238105

   Purpose

   For IVT of wildtype R2Tg editor, codon optimized for mammalian expression.

   Depositor

   Feng Zhang

   Article

   Edmonds et al Nat Commun. 2025 Jul 2;16(1):6079. doi:

   Insert

   Wildtype R2Tg, mammalian codon optimized
   

   Use

   For ivt

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. Nb-gp41-Halo (MoonTag-Nb-Halo)

   Plasmid

   #128603

   Purpose

   Expresses nanobody-2H10 fused to Halo in mammalian cells. It recognizes gp41 peptide array (MoonTag peptide array).

   Depositor

   Marvin Tanenbaum

   Article

   Boersma et al Cell. 2019 Jun 5. pii: S0092-8674(19)304

   Insert

   Nanobody_gp41
   

   Use

   Lentiviral

   Tags

   GB1 and Halo

   Expression

   Mutation

   Promoter

   SFFV

   Available since

   Aug. 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. Hsp90 HA

   Plasmid

   #22487

   Depositor

   William Sessa

   Article

   Garcia-Cardena et al Nature. 1998 Apr 23. 392(6678):821-4.

   Insert

   Hsp90 HA (HSP90AB1 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 20, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3.1-mPCSK2

   Plasmid

   #122673

   Purpose

   Expresses mouse PCSK2 in mammalian cells

   Depositor

   Liming Pei

   Article

   Li et al Mol Cell Biol. 2018 Oct 15;38(21). pii:

   Insert

   Mus musculus proprotein convertase subtilisin/kexin type 2 (Pcsk2) (Pcsk2 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   T7

   Available since

   May 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGL3-NBRE3

   Plasmid

   #208254

   Purpose

   pGL3 promoter luciferase reporter vector containing 3 copies of the NBRE DNA response element (5′-GAGTTTTAAAAGGTCATGCTCAATT TGTC-3′) used for luciferase reporter assays in mammalian cells

   Depositor

   Douglas Kojetin

   Article

   Munoz-Tello et al J Med Chem. 2020 Dec 24;63(24):15639-156

   Insert

   3X NBRE-LUC
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   SV40 early promoter

   Available since

   Feb. 28, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3 flag p53

   Plasmid

   #10838

   Depositor

   Thomas Roberts

   Article

   Gjoerup et al J Virol. 2001 Oct . 75(19):9142-55.

   Insert

   p53 (TP53 Human)
   

   Use

   Tags

   flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 2, 2005

   Availability

   Academic Institutions and Nonprofits only

   

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9. mEmerald-Calreticulin-N-16

   Plasmid

   #54023

   Purpose

   Localization: Endoplasmic Reticulum, Excitation: 487, Emission: 509

   Depositor

   Michael Davidson

   Insert

   Calreticulin (CALR Human)
   

   Use

   Tags

   mEmerald

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 2, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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10. AAV-FLEX-rev-ChR2-tdtomato

    Plasmid

    #18917

    Purpose

    Cre-dependent viral expression of ChR2-tdTomato for neuronal excitation

    Depositor

    Scott Sternson

    Article

    Atasoy et al J Neurosci. 2008 Jul 9. 28(28):7025-30.

    Insert

    Channelrhodopsin 2-tdtomato
    

    Use

    AAV and Cre/Lox; Adeno-associated virus

    Tags

    tdtomato

    Expression

    Mutation

    Promoter

    CAG

    Available since

    Feb. 9, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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11. TetO-FUW-OSKM

    Plasmid

    #20321

    Purpose

    Lentiviral plasmid for tet-inducible expression of mouse Oct4, Sox2, Klf4 and Myc for iPS cell generation

    Depositor

    Rudolf Jaenisch

    Article

    Carey et al Proc Natl Acad Sci U S A. 2009 Jan 6. 10

    Insert

    Oct-4 (Pou5f1, Sox2, Klf4, Myc Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 23, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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12. pEGFP-C1-ADRP

    Plasmid

    #87161

    Purpose

    Fluorescent protein localized to lipid droplets (LDs)

    Depositor

    Elina Ikonen

    Article

    Salo et al EMBO J. 2016 Dec 15;35(24):2699-2716. Ep

    Insert

    ADRP (PLIN2 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA5/FRT/TO_Lifeact_HaloTag9

    Plasmid

    #175545

    Purpose

    Expression of HaloTag9 at the actin filaments of mammalian cells

    Depositor

    Kai Johnsson

    Article

    Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

    Insert

    Lifeact-HaloTag9
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    HaloTag9 = HaloTag7-Q165H-P174R

    Promoter

    Available since

    Dec. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLSB-NAT

    Plasmid

    #166698

    Purpose

    Combined sgRNA/Cas9 pLSB vector with natMX6 (cloNAT) marker. Golden Gate-ready for cloning custom sgRNA sequences.

    Depositor

    Robin Allshire

    Article

    Torres-Garcia et al Wellcome Open Res. 2020 Nov 24;5:274. do

    Inserts

    tRNA promoter : sgRNA cassette
    adh15 promoter : Cas9 codon-optimised for S. pombe
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    adh15 promoter and tRNA promoter

    Available since

    April 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. AAVS1-Puro CAG J591-scFV T-CAR

    Plasmid

    #171961

    Purpose

    Donor plasmid for J591 T-CAR expression in human cells

    Depositor

    Xiaoping Bao

    Article

    Harris et al J Immunol Regen Med. 2023 May;20:100074.

    Insert

    Synthetic J591-scFV CAR
    

    Use

    CRISPR, Synthetic Biology, and TALEN

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    May 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3.1-Cyto-iPASnFR-L-mScarlet3-3xFLAG

    Plasmid

    #225242

    Purpose

    Mammalian expression of iPASnFR-L in the cytosol

    Depositor

    Loren Looger

    Article

    Tamura et al bioRxiv 2024.08.21.609037

    Insert

    iPASnFR-L
    

    Use

    Tags

    3xFLAG and mScarlet3

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCMV4 p65

    Plasmid

    #21966

    Depositor

    Warner Greene

    Article

    Ballard et al Proc Natl Acad Sci U S A. 1992 Mar 1. 89

    Insert

    NFkB p65 subunit (RELA Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    This construct was the product of 3 subcloning pr…

    Promoter

    Available since

    Sept. 17, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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18. pDONR223-EGFR

    Plasmid

    #23935

    Depositor

    David Root, William Hahn

    Article

    Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

    Insert

    EGFR (EGFR Human)
    

    Use

    Gateway donor vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 30, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCMV-PE2-P2A-GFP

    Plasmid

    #132776

    Purpose

    Prime editing in mammalian cells with co-translational GFP expression

    Depositor

    David Liu

    Article

    Anzalone et al Nature. 2019 Oct 21. pii: 10.1038/s41586

    Insert

    PE2-P2A-GFP
    

    Use

    Tags

    SV40 NLS

    Expression

    Mammalian

    Mutation

    See manuscript

    Promoter

    CMV

    Available since

    Jan. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA3-Delta(Pro-MP)ADAM17-HA

    Plasmid

    #65221

    Purpose

    Expression of HA tagged ADAM17 lacking prodomain and metalloprotease domain in mammalian cells

    Depositor

    Axel Ullrich

    Article

    Gschwind et al EMBO J. 2003 May 15;22(10):2411-21.

    Insert

    Delta(Pro-MP)ADAM17 (ADAM17 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    deleted AA 18-473

    Promoter

    CMV

    Available since

    Nov. 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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