159,333 results

1. NG-ABE8e

   Plasmid

   #138491

   Purpose

   A-to-G base editor

   Depositor

   David Liu

   Article

   Richter et al Nat Biotechnol. 2020 Jul;38(7):883-891.

   Insert

   ecTadA(8e)-nSpCas9 (NG)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pF2-DTA

   Plasmid

   #42521

   Depositor

   Terry Magnuson

   Article

   Jang et al PLoS One. 2013;8(2):e57075. doi: 10.1371

   Insert

   EF1a-DTA
   

   Use

   Mouse Targeting

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 14, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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3. HA-Sos1

   Plasmid

   #32920

   Depositor

   Dafna Bar-Sagi

   Article

   Yang et al J Biol Chem. 1995 Aug 4;270(31):18212-5.

   Insert

   SOS1 (SOS1 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 31, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSin-EF2-Sox2-Pur

   Plasmid

   #16577

   Depositor

   James Thomson

   Article

   Yu et al Science. 2007 Dec 21. 318(5858):1917-20.

   Insert

   Homo sapiens SRY (sex determining region Y)-box 2 (SOX2) (SOX2 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 4, 2008

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pCH49 (crRNA expression vector)

   Plasmid

   #217338

   Purpose

   CROP-seq crRNA expression vector with GFP and neo marker

   Depositor

   Luke Gilbert

   Article

   Hsiung et al Nat Biotechnol. 2024 May 17. doi: 10.103

   Insert

   AsDR-filler-AsDR8, GFP-neo
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   hU6

   Available since

   March 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. Stat3 pcDNA3

   Plasmid

   #8706

   Purpose

   See Depositor Comments below for variant information.

   Depositor

   Jim Darnell

   Article

   Zhong et al Proc Natl Acad Sci U S A 1994 May 24;91(

   Insert

   Stat3 (Stat3 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   * see comments

   Promoter

   Available since

   Sept. 26, 2005

   Availability

   Academic Institutions and Nonprofits only

   

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7. gCH128 (non-targeting crRNA)

   Plasmid

   #217339

   Purpose

   Non-targeting crRNA

   Depositor

   Luke Gilbert

   Article

   Hsiung et al Nat Biotechnol. 2024 May 17. doi: 10.103

   Insert

   crNT-3
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   hU6

   Available since

   March 28, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. 3HA-ERHBD-Hoxb8

   Plasmid

   #212932

   Purpose

   Expresses an estrogen-regulated form of Hoxb8 to generate conditionally immortalized hematopoietic progenitor cell lines

   Depositor

   Hans Haecker

   Article

   Redecke et al Nat Methods. 2013 Aug;10(8):795-803. doi

   Insert

   3HA-ERHBD-Hoxb8 (ESR1, Hoxb8 Mouse, Human)
   

   Use

   Retroviral

   Tags

   Triple HA tag

   Expression

   Mammalian

   Mutation

   Promoter

   MSCV LTR

   Available since

   Jan. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAdTrack-CMV

   Plasmid

   #16405

   Purpose

   Shuttle vector for us in AdEasy System. For expression of transgenes under a CMV promoter when a GFP tracer is desired.

   Depositor

   Bert Vogelstein

   Article

   He et al Proc Natl Acad Sci U S A. 1998 Mar 3. 95

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Adenoviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 20, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMXs-IP- SCAT3.2

    Plasmid

    #172497

    Purpose

    SCAT3.2 is fluorescent reporter (FRET probe) for cell survival/apoptosis with Caspase-3 sensitive sequence which can be introduced to mammalian cells by retrovirus vector.

    Depositor

    David Scadden

    Article

    Oki et al Nat Commun. 2021 Jan 11;12(1):245. doi:

    Insert

    Amcyan
    

    Use

    Retroviral

    Tags

    mVenus

    Expression

    Mammalian

    Mutation

    Promoter

    MLV LTR

    Available since

    Aug. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCMV-hARdelta538-614

    Plasmid

    #89107

    Purpose

    mammalian expression of human androgen receptor with deletion of amino acids 538-614, deletion of AR DNA binding domain

    Depositor

    Elizabeth Wilson

    Article

    Zhou et al J Biol Chem. 1994 May 6;269(18):13115-23

    Insert

    hAR-538-614 (AR Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    hAR delta 538-614

    Promoter

    CMV

    Available since

    April 11, 2017

    Availability

    Academic Institutions and Nonprofits only

12. pRDA_887

    Plasmid

    #201164

    Purpose

    Lentiviral expression of EnAsCas12a CRISPRa gRNA with N' nanobody-ALFA and C' p65 and NLS

    Depositor

    John Doench

    Article

    Griffith et al Cell Genom. 2023 Sep 1;3(9):100387. doi:

    Insert

    Puromycin resistance
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EFS

    Available since

    June 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pBTK522-FAST-PETase

    Plasmid

    #214754

    Purpose

    plasmid for expression FAST-PETase in Pseudomonas putida

    Depositor

    Hal Alper

    Article

    Lu et al Nature. 2022 Apr;604(7907):662-667. doi:

    Insert

    FAST-PETase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCMV-hARdeltaNH2-6

    Plasmid

    #89104

    Purpose

    mammalian expression of human androgen receptor amino acids 507-919

    Depositor

    Elizabeth Wilson

    Article

    Simental et al J Biol Chem. 1991 Jan 5;266(1):510-8.

    Insert

    hAR DNA binding domain+ligand binding domain (AR Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    NH2 terminal deletion, contains amino acids 507-…

    Promoter

    CMV

    Available since

    April 18, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pC1-SypHer3s

    Plasmid

    #108118

    Purpose

    Ratiometric pH probe with enhanced brightness for mammalian cells expression.

    Depositor

    Vsevolod Belousov

    Article

    Ermakova et al Chem Commun (Camb). 2018 Mar 2. doi: 10.

    Insert

    SypHer3s
    

    Use

    Tags

    ratiometric YFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLenti CMV Hygro DEST (w117-1)

    Plasmid

    #17454

    Purpose

    3rd gen lentiviral Gateway destination vector, expression, CMV promoter, Hygro

    Depositor

    Paul Kaufman, Eric Campeau

    Article

    Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Lentiviral; Destination vector

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 12, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pET-MBP-mSA2

    Plasmid

    #52319

    Purpose

    Solubly expresses monomeric streptavidin containing S25H mutation in E. coli as an MBP fusion. App Microbiol & Biotech 98, 6285-6295 (2014)

    Depositor

    Sheldon Park

    Article

    Demonte et al Appl Microbiol Biotechnol. 2014;98(14):6

    Insert

    monomeric streptavidin 2
    

    Use

    Tags

    FLAG, His, MBP, and TEV

    Expression

    Bacterial

    Mutation

    S25H mutation compared to streptavidin

    Promoter

    T7

    Available since

    July 15, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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18. GalT-RpHLuorin2

    Plasmid

    #171719

    Purpose

    Trans-Golgi expression of ratiometric pHLuorin2

    Depositor

    Geert van den Bogaart

    Article

    Linders et al ACS Chem Biol. 2022 Jan 21;17(1):240-251

    Insert

    Ratiometric pHLuorin2 fused to B4GALT1 (B4GALT1 Human)
    

    Use

    Tags

    ratiometric pHluorin2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLPT107

    Plasmid

    #85525

    Purpose

    Repressilator with triple reporter

    Depositor

    Johan Paulsson

    Article

    Potvin-Trottier et al Nature. 2016 Oct 12;538(7626):514-517. d

    Inserts

    mCfp
    mVenus
    tetR
    lacI
    cI
    mKate2
    

    Use

    Synthetic Biology

    Tags

    ssrA

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pHCMV_DENV1_PrME

    Plasmid

    #207263

    Purpose

    Mammalian expression of Dengue virus 1 PrME protein

    Depositor

    Feng Zhang

    Article

    Strebinger et al Nat Commun. 2023 Aug 23;14(1):5141. doi:

    Insert

    DENV1_PrME (POLY Dengue virus 1)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    mammalian codon optimization

    Promoter

    CMV promoter

    Available since

    Nov. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge