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pUAST-2x attB

Plasmid

#206362

Purpose

includes two standard UAS cassettes and a third cassette without a promoter

Depositor

Erdem Bangi

Article

Quintero et al Cell Rep. 2023 Nov 28;42(11):113370. doi

Type

Empty backbone

Expression

Insect

Available Since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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EF1a_SOX10_P2A_Hygro_Barcode

Plasmid

#120479

Purpose

Barcoded lentiviral vector to express SOX10 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

Depositor

Prashant Mali

Article

Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

Insert

SOX10 (SOX10 Human)

Use

Lentiviral

Promoter

EF1a

Available Since

Feb. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3 GFP LIC cloning vector (6D)

Plasmid

#30127

Depositor

Scott Gradia

Type

Empty backbone

Tags

GFP

Expression

Mammalian

Available Since

June 9, 2011

Availability

Academic Institutions and Nonprofits only

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pEntry_HCoV-OC43-Nucleocapsid

Plasmid

#168947

Purpose

Gateway-compatible Entry vector

Depositor

Pascal Falter-Braun

Article

Weller et al G3 (Bethesda). 2023 Jul 5;13(7):jkad105.

Insert

HCoV-OC43-Nucleocapsid (EYW02_gp8 )

Use

Gateway-compatible entry vector

Available Since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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pCAGGS_hTRPV1-p2A-tdTomato

Plasmid

#238923

Purpose

Expression of the human TRPV1 channel with tdTomato as a reporter in mammalian cells

Depositor

Vsevolod Belousov

Article

Maltsev et al Cell Mol Life Sci. 2024 Oct 25;81(1):437

Insert

hTRPV1-p2A-tdTomato

Expression

Mammalian

Promoter

CAG

Available Since

Aug. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pGL3 2 kb prom. CD274

Plasmid

#107003

Purpose

Modified Luciferase expression vector

Depositor

Julian Downward

Article

Coelho et al Immunity. 2017 Dec 19;47(6):1083-1099.e6

Insert

CD274 (CD274 Human)

Use

Luciferase

Expression

Mammalian

Available Since

March 23, 2018

Availability

Academic Institutions and Nonprofits only

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pJT258 Lenti pEF-dCas9-3XFLAG-N+F+Z-T2A-tagBFP-P2A-Blast

Plasmid

#187334

Purpose

dCas9 effector fusion for manipulating transcription

Depositor

Michael Bassik, Lacra Bintu

Article

Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

Insert

dCas9-3XFLAG-N+F+Z-T2A-tagBFP-P2A-Blast

Use

CRISPR and Lentiviral

Tags

3XFLAG

Expression

Mammalian

Promoter

pEF1a

Available Since

May 6, 2024

Availability

Academic Institutions and Nonprofits only

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mNeonGreen-peroxi

Plasmid

#137806

Purpose

Visualization of peroxisomes

Depositor

Dorus Gadella

Article

Chertkova et al bioRxiv (2020) 160374; doi: https://doi.

Insert

SKL

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV_hsyn_NES-his-CaMPARI2-WPRE-SV40

Plasmid

#101060

Purpose

AAV vector expressing CaMPARI2 (Kd = 200nM), a photoconvertible fluorescent protein-based calcium integrator, in neurons

Depositor

Eric Schreiter

Article

Moeyaert et al Nat Commun. 2018 Oct 25;9(1):4440. doi:

Has Service

AAV1

Insert

CaMPARI2

Use

AAV

Tags

FLAG-HA-myc and NES_his

Promoter

hsyn (synapsin-1)

Available Since

Oct. 2, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3.2-Cx43-HA

Plasmid

#49851

Purpose

Encodes human connexin 43 with a C-terminal HA epitope fusion tag

Depositor

Robin Shaw

Article

Smyth et al Cell Rep. 2013 Nov 5. pii: S2211-1247(13

Insert

Connexin 43 (GJA1 Human)

Tags

HA

Expression

Mammalian

Mutation

Contains several wobble mutations to confer resis…

Promoter

CMV

Available Since

Jan. 17, 2014

Availability

Academic Institutions and Nonprofits only

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pET21a(+)-Histag-mCherry (527)

Plasmid

#70719

Purpose

Expression vector for mCherry purification

Depositor

Richard Murray

Article

Niederholtmeyer et al Elife. 2015 Oct 2;4. doi: 10.7554/eLife.

Insert

mCherry

Available Since

Feb. 2, 2016

Availability

Academic Institutions and Nonprofits only

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AB-GFP

Plasmid

#72203

Purpose

screen AB42 variants for aggregation

Depositor

Michael Hecht

Article

Wurth et al J Mol Biol. 2002 Jun 21;319(5):1279-90.

Insert

AB42 (APP Human)

Tags

GFP

Expression

Bacterial

Promoter

T7

Available Since

Dec. 10, 2015

Availability

Academic Institutions and Nonprofits only

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pLV-EF1-Cre-PGK-Puro

Plasmid

#108543

Purpose

Lentivirus vector expessing Cre under EF1a promoter and puromycin under PGK promoter

Depositor

Javier Alcudia

Inserts

cre recombinase
Puromycin

Promoter

EF1apha and PGK

Available Since

April 19, 2018

Availability

Academic Institutions and Nonprofits only

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pLX304_CMV::mCherry-LgBiT-FLAG-NES

Plasmid

#199715

Purpose

Expresses LgBiT, fused to mCherry, in the cytosol of mammalian cells. Can be used in generating stable cell lines via lentiviral transduction.

Depositor

Alice Ting

Article

Roh et al J Am Chem Soc. 2023 May 10;145(18):10220

Insert

mCherry-LgBiT-FLAG-NES

Use

Lentiviral and Luciferase

Expression

Mammalian

Promoter

CMV

Available Since

May 9, 2023

Availability

Academic Institutions and Nonprofits only

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pTol1-UAS:ChrimsonR-tdTomato

Plasmid

#124231

Purpose

Zebrafish Tol1 construct for UAS-mediated expression of the optogenetic opsin ChrimsonR fused to the red fluorescent protein tdTomato

Depositor

Isaac Bianco

Article

Antinucci et al Elife. 2020 Mar 27;9. pii: 54937. doi: 1

Insert

ChrimsonR-tdTomato

Use

Zebrafish expression

Tags

tdTomato

Promoter

14xUAS-E1b

Available Since

Jan. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-mCherry

Plasmid

#114472

Purpose

mCherry Control

Depositor

Karl Deisseroth

Has Service

AAV Retrograde, AAV1, AAV2, AAV5, AAV8, and AAV9

Insert

mCherry

Use

AAV

Promoter

hSyn

Available Since

Sept. 5, 2018

Availability

Academic Institutions and Nonprofits only

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pQCXIP-cathepsinD-YMH

Plasmid

#182644

Purpose

Mammalian expression vector encoding a protein fusion composed of human cathepsin D fused to to one tyrosine sulfation (TS) motif (Y), myc (M) and His6 (H) epitope

Depositor

Martin Spiess

Article

Buser et al Life Sci Alliance. 2022 Mar 21;5(7). pii

Insert

cathepsinD

Use

Retroviral

Tags

His6-tag, TS site from proCCK, and myc-tag

Expression

Mammalian

Promoter

CMV

Available Since

April 22, 2022

Availability

Academic Institutions and Nonprofits only

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pAKlux2

Plasmid

#14080

Depositor

Attila Karsi

Article

Karsi et al Plasmid. 2007 May;57(3):286-95. doi: 10.

Insert

Bacterial luciferase

Expression

Bacterial

Promoter

Lac, T3

Available Since

March 12, 2007

Availability

Academic Institutions and Nonprofits only

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pMpGE013

Plasmid

#108681

Purpose

All-in-one genome editing vector in M. polymorpha (hygromycin resistant)

Depositor

Takayuki Kohchi, Shigeo Sugano

Article

Sugano et al PLoS One. 2018 Oct 31;13(10):e0205117. d

Type

Empty backbone

Use

CRISPR

Available Since

June 1, 2018

Availability

Academic Institutions and Nonprofits only

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HA-APP-mGFP

Plasmid

#196696

Purpose

Expresses APP695 with an HA tag an N-terminal for single molecule tracking and other measusers in mammalian cells

Depositor

Martino Calamai

Article

Capitini et al iScience. 2023 Apr 11;26(5):106611. doi:

Insert

Amyloid Precursor Protein 695 isoform (APP Human)

Tags

HA and mGFP

Expression

Mammalian

Available Since

April 26, 2023

Availability

Academic Institutions and Nonprofits only

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