161,709 results

1. pCW57.1_AOX-FLAG

   Plasmid

   #177984

   Purpose

   All-in-one dox-inducible expression of Alternative Oxidase

   Depositor

   Jessica Spinelli, David Sabatini

   Article

   Spinelli et al Science. 2021 Dec 3;374(6572):1227-1237.

   Insert

   AOX
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Codon optimized cDNA for expression in human cells

   Promoter

   TRE

   Available since

   Dec. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCHAC-mt-mKeima

   Plasmid

   #72342

   Purpose

   retrovirus construct for stable expressing mt-mKeima

   Depositor

   Richard Youle

   Article

   Lazarou et al Nature. 2015 Aug 20;524(7565):309-14. do

   Insert

   mt-mKeima
   

   Use

   Retroviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 8, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCMV-ClpX

   Plasmid

   #234733

   Purpose

   Human-codon optimized E. coli ClpX in mammalian cells

   Depositor

   David Liu

   Article

   Witte et al Science. 2025 May 15;388(6748):eadt5199.

   Insert

   ClpX
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. MC4R-Tango

   Plasmid

   #66430

   Purpose

   Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

   Depositor

   Bryan Roth

   Article

   Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

   Insert

   MC4R (MC4R Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 15, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLKO.1-puro-shNT

   Plasmid

   #109012

   Purpose

   short-hairpin knockdown of targeted gene

   Depositor

   Jacob Corn

   Article

   Liang et al J Cell Biol. 2018 Aug 24. pii: jcb.20180

   Insert

   Non-targeting shRNA
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6 promoter

   Available since

   June 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pB-mTSPAN4-mCherry

   Plasmid

   #210330

   Purpose

   Overexpression of mTSPAN4

   Depositor

   Li Yu

   Article

   Jiao et al Cell. 2021 May 27;184(11):2896-2910.e13.

   Insert

   mTSPAN4 (Tspan4 Mouse)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 28, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSI-Check2-hRluc-NFkB-firefly

   Plasmid

   #106979

   Purpose

   Luciferase reporter with internal control for NFkB signal.

   Depositor

   Qing Deng

   Article

   Zhou et al Cell Rep. 2018 Feb 13;22(7):1810-1823. d

   Insert

   NFKB recognition sites (NFKB1 Human)
   

   Use

   Luciferase

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 25, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pJRH-1179 U6-reci Gag-Cas9 v2

   Plasmid

   #201914

   Purpose

   Cas9-EDV production plasmid. Expresses the v2 Gag-Cas9 polypeptide (CAG promoter) and sgRNA (human U6 promoter. BsmBI cutsites for spacer insertion)

   Depositor

   Jennifer Doudna

   Article

   Hamilton et al Nat Biotechnol. 2024 Jan 11. doi: 10.103

   Insert

   Gag-Cas9 v2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Aug. 4, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGEM4Z-EGFP

   Plasmid

   #183475

   Purpose

   This plasmid can be used to prepare EGFP mRNA by In Vitro Transcription

   Depositor

   Chantal Pichon

   Article

   Perche et al Mol Ther Nucleic Acids. 2019 Sep 6;17:76

   Insert

   EGFP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   July 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCMV enAsCas12f-YHAM-sgRNA_v7

    Plasmid

    #208356

    Purpose

    Expression of enAsCas12f-YHAM and sgRNA_v7 in mammalian cells

    Depositor

    Atsushi Hoshino

    Article

    Hino et al Cell. 2023 Sep 22:S0092-8674(23)00963-7.

    Insert

    enAsCas12f-YHAM
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    F48Y/S188H/V232A/E316M

    Promoter

    CMV

    Available since

    Oct. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. lenti-sgRNA blast

    Plasmid

    #104993

    Purpose

    This 3rd generation lentiviral plasmid expresses a S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from a U6 promoter and blasticidin S resistance from an EF-1a promoter.

    Depositor

    Brett Stringer

    Article

    Stringer et al Sci Rep. 2019 Mar 20;9(1):4902. doi: 10.

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. HyPer7.2DAAO-NES

    Plasmid

    #168301

    Purpose

    Chemogenetic fluorescent H2O2 reporter targeted to the cytoplasm

    Depositor

    Thomas Michel

    Article

    Saeedi Saravi et al Redox Biol. 2020 Sep;36:101605. doi: 10.

    Insert

    HyPer7.2DAAO-NES
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pHR-TetOn-mCherry-OAZ1_FS-YFP

    Plasmid

    #232356

    Purpose

    Mammalian expression of dox-inducible polyamine sensor (polyamine levels = YFP/Cherry)

    Depositor

    Ankur Jain

    Article

    Sharma et al bioRxiv [Preprint]. 2024 Nov 17:2024.08.

    Insert

    OAZ1 derived polyamine sensing module (OAZ1 Human)
    

    Use

    Lentiviral

    Tags

    eYFP and mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    TRE3G

    Available since

    March 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLenti-EGFP-2xFYVE

    Plasmid

    #136996

    Purpose

    Lentiviral EGFP lipid sensor for PI(3)P

    Depositor

    Ken-Ichi Takemaru

    Insert

    EGFP-2xFYVE
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mutation

    Promoter

    EF1 alpha

    Available since

    Feb. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pWPXL

    Plasmid

    #12257

    Purpose

    2nd generation lentiviral transfer plasmid

    Depositor

    Didier Trono

    Type

    Empty backbone

    Use

    Cre/Lox and Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 4, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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16. pTol1-UAS:CoChR-tdTomato

    Plasmid

    #124233

    Purpose

    Zebrafish Tol1 construct for UAS-mediated expression of the optogenetic opsin CoChR fused to the red fluorescent protein tdTomato

    Depositor

    Isaac Bianco

    Article

    Antinucci et al Elife. 2020 Mar 27;9. pii: 54937. doi: 1

    Insert

    CoChR-tdTomato
    

    Use

    Zebrafish expression

    Tags

    tdTomato

    Expression

    Mutation

    Promoter

    14xUAS-E1b

    Available since

    Jan. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLV UBC hTMEM115-3C-3xHA-P2A-eGFP

    Plasmid

    #214996

    Purpose

    Stably express the GolgiTag (GolgiIP handle) in mammalian cells (human ORF). eGFP for cell sorting desired expression levels.

    Depositor

    Wade Harper

    Article

    Hollingsworth et al bioRxiv 2024.04.19.590338

    Insert

    TMEM115 (TMEM115 Human)
    

    Use

    Lentiviral

    Tags

    3xHA

    Expression

    Mammalian

    Mutation

    Promoter

    UBC

    Available since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pDONR221-SLC7A5_STOP

    Plasmid

    #161363

    Purpose

    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.

    Depositor

    RESOLUTE Consortium, Giulio Superti-Furga

    Article

    Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

    Insert

    SLC7A5 (SLC7A5 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 15, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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19. pMSP1D1

    Plasmid

    #20061

    Purpose

    Bacterial expression of membrane scaffold protein (MSP1D1) for Nanodisc formation

    Depositor

    Stephen Sligar

    Article

    Denisov et al J Am Chem Soc. 2004 Mar 24. 126(11):3477

    Insert

    MSP1D1
    

    Use

    Tags

    7-His tag

    Expression

    Bacterial

    Mutation

    deletion mutant (1-11) of MSP1; His-tag on N-term…

    Promoter

    Available since

    March 16, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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20. lenti sgRNA(MS2)_zeo backbone

    Plasmid

    #61427

    Purpose

    3rd generation lenti sgRNA cloning backbone with MS2 loops at tetraloop and stemloop 2 and EF1a-zeo resistance marker. Contains BsmBI sites for insertion of spacer sequences.

    Depositor

    Feng Zhang

    Article

    Konermann et al Nature. 2014 Dec 10. doi: 10.1038/nature

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 and EF1A

    Available since

    Dec. 15, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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