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qTAG-C-ultraID-Blast

Plasmid

#207715

Purpose

Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a ultraID-2A-Blast cassette

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Type

Empty backbone

Use

CRISPR; Donor cassette

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 15, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pCS2-Arl13b-mCardinal

Plasmid

#194542

Purpose

Expresses Arl13b with mCardinal in mammalian cells and zebrafish embryos

Depositor

Heidi Hehnly

Article

Aljiboury et al PLoS Genet. 2023 May 15;19(5):e1010765.

Insert

Arl13b (Arl13b Mouse)

Use

Tags

mCardinal

Expression

Mammalian

Mutation

Promoter

CMV IE94

Available since

Jan. 17, 2023

Availability

Academic Institutions and Nonprofits only

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Tet1-dCas9

Plasmid

#136650

Purpose

Tet1 fused to N-terminus of dCas9; pCDNA3 vector backbone, mammalian expression

Depositor

David Segal

Article

Umeh-Garcia et al Br J Cancer. 2022 Apr 19. pii: 10.1038/s

Insert

Tet1 (Tet1 Mouse)

Use

CRISPR

Tags

3XFLag-NLS-Tet1-dCas9-NLS

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 19, 2021

Availability

Academic Institutions and Nonprofits only

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EGFP-GRAM1b

Plasmid

#211494

Purpose

Expression of EGFP-tagged GRAM domain of GRAMD1b (EGFP-GRAM1b) in mammalian cells

Depositor

Yasunori Saheki

Article

Naito et al Elife. 2019 Nov 14;8. pii: 51401. doi: 1

Insert

GRAM domain of GRAMD1b/Aster-B (GRAMD1B Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-M13-TEV-C-P2A-TdTomato

Plasmid

#92391

Purpose

produces AAV expressing Cal-light M13-TevC component and TdTomato

Depositor

Hyungbae Kwon

Article

Lee et al Nat Biotechnol. 2017 Jun 26. doi: 10.103

Insert

M13-TEV-C-P2A-TdTomato

Use

AAV and Synthetic Biology

Tags

TdTomato

Expression

Mammalian

Mutation

Promoter

human synapsin

Available since

June 26, 2017

Availability

Academic Institutions and Nonprofits only

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pAAV/mIba1.GFP.WPRE.miR-9.T.miR-129-2-3p.T.SV40pA

Plasmid

#190163

Purpose

Microglia-targeted transgene expression.

Depositor

Hirokazu Hirai

Article

Okada et al Commun Biol. 2022 Nov 11;5(1):1224. doi:

Inserts

mIba1 promoter, 1.7-kb, microglia-specific promoter
miR-9-Tx4, 4 repeated miRNA target sites for miR-9
miR-129-2-3p-Tx4, 4 repeated miRNA target sites for miR-129-2-3p

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

Nov. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pKL2299A

Plasmid

#222105

Purpose

A ternary vir helper plasmid that carries Agrobacterium tumefaciens virulence genes for enhanced plant genetic transformation.

Depositor

Kan Wang

Article

Aliu et al Front Plant Sci. 2024 Jul 23;15:1429353.

Insert

virA

Use

Tags

Expression

Bacterial and Plant

Mutation

Promoter

virA promoter

Available since

Oct. 1, 2024

Availability

Academic Institutions and Nonprofits only

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MSGV1-1D3-28Z.1-3 mut

Plasmid

#107227

Purpose

Anti-mouse CD19 CAR with CD3 ITAMs mutated

Depositor

James Kochenderfer, Steven Rosenberg

Article

Kochenderfer et al Blood. 2010 Nov 11;116(19):3875-86. doi:

Insert

1D3-28Z. 1-3 mut

Use

Retroviral

Tags

None

Expression

Mutation

None

Promoter

Available since

March 30, 2018

Availability

Academic Institutions and Nonprofits only

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pHRdSV40-NLS-dCas9-24xGCN4_v4-NLS-P2A-BFP-dWPRE

Plasmid

#60910

Purpose

dCas9 fused to 24 copies of the GCN4 peptide v4, which is part of the SunTag system

Depositor

Ron Vale

Article

Tanenbaum et al Cell. 2014 Oct 8. pii: S0092-8674(14)012

Insert

dCas9

Use

CRISPR and Lentiviral

Tags

2x NLS, HA, and NLS

Expression

Mammalian

Mutation

Promoter

dSV40 Promoter

Available since

Nov. 18, 2014

Availability

Academic Institutions and Nonprofits only

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p4455 FLAG-hPLIC-2

Plasmid

#8661

Depositor

Peter Howley

Article

Kleijnen et al Mol Cell 2000 Aug;6(2):409-19.

Insert

hPLIC-2 (UBQLN2 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

Available since

June 16, 2005

Availability

Academic Institutions and Nonprofits only

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pET21-pelB-LaM-4

Plasmid

#172770

Purpose

Bacterial expression of anti-mCherry nanobody LaM-4, with pelB leader and C-term free cysteine and 6xHIS tag.

Depositor

Michael Rout

Article

Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

Insert

LaM-4

Use

Tags

6xHIS and pelB leader for periplasmic secretion

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 20, 2021

Availability

Academic Institutions and Nonprofits only

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P4-PE

Plasmid

#211375

Purpose

Packaging plasmid for v3b PE-eVLP production

Depositor

David Liu

Article

An et al Nat Biotechnol. 2024 Jan 8. doi: 10.1038

Insert

P4-PE

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 12, 2024

Availability

Academic Institutions and Nonprofits only

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YFP NLS Beta-Actin

Plasmid

#60613

Purpose

Encodes YFP and NLS tagged beta-actin

Depositor

Primal de Lanerolle

Article

Chang et al Proc Natl Acad Sci U S A. 2011 May 24;10

Insert

Beta-actin (ACTB Human)

Use

Tags

EYFP and NLS

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 21, 2014

Availability

Academic Institutions and Nonprofits only

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pLG005 (Retron-TIR)

Plasmid

#157883

Purpose

Expresses the retron-TIR defense system

Depositor

Feng Zhang

Article

Gao et al Science. 2020 Aug 28;369(6507):1077-1084

Insert

Retron-TIR

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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ABEmax-Puro V3

Plasmid

#226956

Purpose

ABEmax plasmid enabling A:T to G:C base editing using a single plasmid. sgRNA uses 3TC scaffold for increased sgRNA expression.

Depositor

Paul Thomas

Article

Chey et al BMC Genomics. 2025 Feb 12;26(1):138. doi

Type

Empty backbone

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 10, 2025

Availability

Academic Institutions and Nonprofits only

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pEGFP-coilin

Plasmid

#36906

Depositor

Greg Matera

Article

Shpargel et al J Cell Sci. 2003 Jan 15;116(Pt 2):303-12

Insert

coilin (COIL Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 22, 2013

Availability

Academic Institutions and Nonprofits only

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pCytERM_mScarlet_N1

Plasmid

#85066

Purpose

In vivo visualization of the ER (can be used for colocalization studies and the OSER assay)

Depositor

Dorus Gadella

Article

Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

Insert

CytERM

Use

Tags

mScarlet

Expression

Mammalian

Mutation

aa1-29

Promoter

CMV

Available since

Dec. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pECFP-Sec31A

Plasmid

#66612

Purpose

CFP fusion of Sec31A

Depositor

David Stephens

Article

Stephens et al J Cell Sci. 2000 Jun;113 ( Pt 12):2177-8

Insert

Sec31A (SEC31A Human)

Use

Tags

ECFP

Expression

Mammalian

Mutation

Promoter

Available since

July 8, 2015

Availability

Academic Institutions and Nonprofits only

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pSG5-BRLF1

Plasmid

#72635

Purpose

Mammalian expression vector for Epstein-Barr virus Rta immediate-early replication and transcription activator

Depositor

S. Diane Hayward

Article

Sarisky et al J Virol. 1996 Dec;70(12):8340-7.

Insert

BRLF1

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 11, 2016

Availability

Academic Institutions and Nonprofits only
pAAV-U6/sgClta-sfGFP-Clta

Plasmid

#200261

Purpose

U6 promoter expressing sgClta. Clta homology arms, sfGFP knock-in.

Depositor

Justin Eyquem

Article

Nyberg et al Cell. 2023 Jan 19;186(2):446-460.e19. do

Insert

sfGFP flanked by homology arms targeting Clta, additional U6 promoter expressing sgClta

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

Dec. 13, 2023

Availability

Academic Institutions and Nonprofits only

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