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pHRIG-Akt1
(Plasmid #53583)

Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 53583 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Stbl3
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    cAkt1
  • Alt name
    Akt1
  • Alt name
    thymoma viral proto-oncogene 1
  • Species
    H. sapiens (human)
  • Mutation
    constitutively active (myristoylated version)
  • Entrez Gene
    AKT1 (a.k.a. AKT, CWS6, PKB, PKB-ALPHA, PRKBA, RAC, RAC-ALPHA)
  • Promoter CMV
  • Tags / Fusion Proteins
    • HA (C terminal on insert)
    • IRES-EGFP (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SalI (not destroyed)
  • 3′ cloning site SacII (not destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer pCDH-rev
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

cAkt1-HA (pcDNA3.1) provided by Dr Richard Roth, Department of Chemical and Systems Biology, Stanford University.

cAkt1 was fused with a Gag polypeptide and lacks the pleckstrin homology (PH) domain; the expression of a myristoylation signal enables Akt1 protein to attach to the cell membrane and become constitutively phosphorylated, thus activated (Aimee 1996).

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pHRIG-Akt1 was a gift from Heng Zhao (Addgene plasmid # 53583 ; http://n2t.net/addgene:53583 ; RRID:Addgene_53583)
  • For your References section:

    Akt isoforms differentially protect against stroke-induced neuronal injury by regulating mTOR activities. Xie R, Cheng M, Li M, Xiong X, Daadi M, Sapolsky RM, Zhao H. J Cereb Blood Flow Metab. 2013 Dec;33(12):1875-85. doi: 10.1038/jcbfm.2013.132. Epub 2013 Aug 14. 10.1038/jcbfm.2013.132 PubMed 23942361