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Engineering an improved light-induced dimer (iLID) for controlling the localization and activity of signaling proteins.
Guntas G, Hallett RA, Zimmerman SP, Williams T, Yumerefendi H, Bear JE, Kuhlman B
Proc Natl Acad Sci U S A. 2015 Jan 6;112(1):112-7. doi: 10.1073/pnas.1417910112. Epub 2014 Dec 22.
PubMed Article

Plasmids from Article

ID Plasmid Purpose
60408pQE-80L iLID (C530M)E. coli expression of iLID (C530M)
60409pQE-80L MBP-SspB NanoE. coli expression of SspB Nano
60410pQE-80L MBP-SspB MicroE. coli expression of SspB Micro
60411pLL7.0: Venus-iLID-CAAX (from KRas4B)Mammalian Expression of Venus-iLID-CAAX
60412pLL7.0: Venus-oLID-CAAX (from KRas4B)Mammalian Expression of Venus-oLID-CAAX
60413pLL7.0: Venus-iLID-Mito (From ActA)Mammalian Expression of Venus-iLID-Mito
60414pLL7.0: Venus-oLID-Mito (From ActA)Mammalian Expression of Venus-oLID-Mito
60415pLL7.0: tgRFPt-SSPB WTMammalian Expression of tgRFPt-Nano
60416pLL7.0: tgRFPt-SSPB R73QMammalian Expression of tgRFPt-Micro
60418pLL7.0: mTiam1(64-437)-tgRFPt-SSPB R73QMammalian Expression of Tiam DH/PH-tgRFPt-Micro
60419pLL7.0: hITSN1(1159-1509)-tgRFPt-SSPB WTMammalian Expression of ITSN DH/PH-tgRFPt-Nano
60420pLL7.0: hITSN1(1159-1509)-tgRFPt-SSPB R73QMammalian Expression of ITSN DH/PH-tgRFPt-Micro

Antibodies from Article