165,886 results

1. pWM_12x601_30bpLinker

   Plasmid

   #157789

   Purpose

   EcoRV digestion produces 12x601 chromatin assembly construct with 30 bp linker lengths in addition to carrier DNA that prevents overassembly of nucleosomes

   Depositor

   Michael Rosen

   Article

   Gibson et al Cell. 2019 Oct 3;179(2):470-484.e21. doi

   Insert

   12x601_30bp linker
   

   Expression

   Bacterial

   Available Since

   March 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBAD-bARGSer-AxeTxe

   Plasmid

   #192473

   Purpose

   Second-generation bacterial acoustic reporter gene derived from Serratia

   Depositor

   Mikhail Shapiro

   Article

   Hurt et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

   Inserts

   bARGSer
   AxeTxe
   

   Expression

   Bacterial

   Mutation

   the gene Ser39006_001280 was deleted from the clu…

   Promoter

   Native promoter from Enterococcus faecium and pBAD

   Available Since

   Jan. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. BASU RaPID plasmid

   Plasmid

   #107250

   Purpose

   Expresses the BASU RaPID labeling protein

   Depositor

   Paul Khavari

   Article

   Ramanathan et al Nat Methods. 2018 Feb 5. pii: nmeth.4601

   Insert

   BASU
   

   Use

   Lentiviral

   Tags

   HA

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 23, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. hCathepsin S

   Plasmid

   #11251

   Purpose

   Mammalian expression of human Cathepsin S

   Depositor

   Hyeryun Choe

   Article

   Huang et al J Biol Chem. 2006 Feb 10;281(6):3198-203

   Insert

   cathepsin S (CTSS Human)
   

   Tags

   C9 (GTETSQVAPA)

   Expression

   Mammalian

   Available Since

   Aug. 4, 2006

   Availability

   Industry, Academic Institutions, and Nonprofits

5. pET16better_H10_HRV3C_chitinase

   Plasmid

   #175091

   Purpose

   Expresses B. licheniformis chitinase with an N terminal 10X His tag and HRV-3C cleavage site in E. coli.

   Depositor

   Jay C Dunlap

   Article

   Bartholomai et al Fungal Genet Biol. 2021 Aug 20;156:10361

   Insert

   chitinase
   

   Tags

   10X His, HRV-3C

   Expression

   Bacterial

   Available Since

   Oct. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pEGFP×2-N1

   Plasmid

   #86775

   Purpose

   A mammalian expression vector with double EGFP at C-terminus.

   Depositor

   Lei Lu

   Article

   Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393

   Type

   Empty backbone

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pT22-GluCNZ-6His

   Plasmid

   #213504

   Purpose

   Designed for cytoplasmic E.coli overexpression of glucanase (β-1,3-glycosidase) for gentle yeast spheroblasting that unlike Zymolyase prevents proteolysis of intracellular components.

   Depositor

   John Denu

   Article

   Kuznetsov et al J Biol Chem. 2018 Aug 3;293(31):12271-12

   Insert

   Glucanase (glucan endo-1,3-beta-glucosidase)
   

   Tags

   6His tag

   Expression

   Bacterial

   Mutation

   1-36 Signal Sequence Deletion

   Promoter

   T7

   Available Since

   March 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. His6_MBP_FMRP_P451S

   Plasmid

   #198693

   Purpose

   Expression of human FMRP protein in E. coli

   Depositor

   Simpson Joseph

   Article

   Edwards et al Sci Rep. 2020 Sep 28;10(1):15858. doi: 1

   Insert

   FMR1 (FMR1 Human)
   

   Tags

   MBP

   Expression

   Bacterial

   Mutation

   changed proline 451 to serine

   Promoter

   T7

   Available Since

   May 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCDNA3 Flag Jnk1a1

   Plasmid

   #13798

   Depositor

   Roger Davis

   Article

   Derijard et al Cell. 1994 Mar 25. 76(6):1025-37.

   Insert

   Jnk1a1 (MAPK8 Human)
   

   Tags

   Flag

   Expression

   Mammalian

   Available Since

   Feb. 22, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSmac-GFP

    Plasmid

    #40881

    Depositor

    Douglas Green

    Article

    Tait et al Dev Cell. 2010 May 18;18(5):802-13.

    Insert

    Smac-GFP (DIABLO Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 9, 2012

    Availability

    Academic Institutions and Nonprofits only

11. hCas9

    Plasmid

    #41815

    Purpose

    Expresses human codon optimized Cas9 nuclease for genome engineering

    Depositor

    George Church

    Article

    Mali et al Science. 2013 Feb 15;339(6121):823-6. do

    Insert

    Cas9
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    human codon-optimized

    Promoter

    CMV

    Available Since

    Jan. 11, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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12. GFP- SEC61B

    Plasmid

    #121159

    Purpose

    Mammalian expression of GFP tagged SEC61B, used as a general ER marker

    Depositor

    Christine Mayr

    Article

    Ma et al Cell. 2018 Oct 30. pii: S0092-8674(18)31

    Insert

    SEC61B (SEC61B Human)
    

    Expression

    Mammalian

    Available Since

    Feb. 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. p3xFLAG SARS-CoV2 RdRp.co

    Plasmid

    #154008

    Purpose

    Expression of codon-optimized SARS-CoV2 Nsp12 (RdRp) in mammalian cells

    Depositor

    Patrick Moore

    Article

    Meng et al J Med Virol. 2023 Jan;95(1):e28246. doi:

    Insert

    Codon-optimized SARS-CoV2 Nsp12 from ORF1ab (ORF1ab Synthetic)
    

    Tags

    3xFLAG tag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 23, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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14. pMSCVpuro-Venus

    Plasmid

    #96940

    Purpose

    Retroviral plasmid containing Venus for labeling of cells

    Depositor

    Lee Grimes

    Article

    Kleeman et al Cytometry A. 2018 Mar 13. doi: 10.1002/c

    Insert

    Venus Fluorescent Protein
    

    Expression

    Mammalian

    Available Since

    April 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAY15 (OsTIR1(F74G) mAID-EGFP-NLS)

    Plasmid

    #140534

    Purpose

    OsTIR1(F74G)-P2A-mAID-EGFP-NLS TOL2

    Depositor

    Masato Kanemaki

    Article

    Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

    Insert

    OsTIR1(F74G) mAID-EGFP-NLS TOL2
    

    Expression

    Mammalian

    Available Since

    Nov. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pVITRO-HPV33 L1L2

    Plasmid

    #52493

    Purpose

    Expresses HPV33 L1 and L2 in mammalian cells

    Depositor

    Richard Roden

    Article

    Kwak et al PLoS One. 2014 May 9;9(5):e97232. doi: 1

    Inserts

    HPV33 L1
    HPV33 L2
    

    Expression

    Mammalian

    Available Since

    May 20, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCMV-TadA8e-BLM

    Plasmid

    #221226

    Purpose

    Express HACE Editor as a fusion of TadA-8e adenosine deaminase and BLM helicase.

    Depositor

    Fei Chen

    Article

    Chen et al Science. 2024 Oct 11;386(6718):eadn5876.

    Insert

    TadA8e-BLM
    

    Expression

    Mammalian

    Available Since

    Sept. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. T7-VEE-IRES-Puro

    Plasmid

    #58970

    Purpose

    noninfectious (non-packaging), self-replicating Venezuelan equine encephalitis (VEE) virus RNA replicon

    Depositor

    Steven Dowdy

    Article

    Yoshioka et al Cell Stem Cell. 2013 Aug 1;13(2):246-54.

    Type

    Empty backbone

    Use

    Venezuelan equine encephalitis (vee) virus rna re…

    Expression

    Mammalian

    Promoter

    26S subgenomic promoter

    Available Since

    Oct. 2, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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19. pDONR223_TMPO_WT

    Plasmid

    #81818

    Purpose

    Gateway Donor vector containing TMPO , part of the Target Accelerator Plasmid Collection.

    Depositor

    Jesse Boehm, William Hahn, David Root

    Article

    Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

    Insert

    TMPO (TMPO Human)
    

    Use

    Gateway entry vector

    Mutation

    WT

    Promoter

    None

    Available Since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pXG237

    Plasmid

    #141281

    Purpose

    ATF4 translational reporter

    Depositor

    Martin Kampmann

    Article

    Guo et al Nature. 2020 Mar;579(7799):427-432. doi:

    Insert

    uORF1/2 of ATF4 followed by mApple
    

    Use

    Lentiviral

    Tags

    mApple

    Promoter

    CMV

    Available Since

    Aug. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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