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pBabe_Hyg_cOVA_T2A_mStrawberry

Plasmid

#161737

Purpose

Expression of cOVA and mStrawberry

Depositor

Alec Kimmelman

Article

Yamamoto et al Nature. 2020 May;581(7806):100-105. doi:

Insert

cytoplasmic ovalbumin

Use

Retroviral

Tags

mStrawberry

Expression

Mammalian

Available Since

Dec. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pBetaActin-Halo-CAMSAP3

Plasmid

#191329

Purpose

Expresses the fluorescently labeled microtubule minus end marker CAMSAP3 to measure microtubule retrograde flow

Depositor

Frank Bradke

Article

Schelski et al Sci Adv. 2022 Nov 4;8(44):eabo2336. doi:

Insert

Halo-CAMSAP3 (Camsap3 Mouse)

Expression

Mammalian

Available Since

Jan. 12, 2023

Availability

Academic Institutions and Nonprofits only

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pYLsgRNA-AtU3d

Plasmid

#66200

Purpose

cloning of sgRNAs for expression in plants

Depositor

Yao-Guang Liu

Article

Ma et al Mol Plant. 2015 Apr 24. pii: S1674-2052(

Type

Empty backbone

Use

Cloning vector

Promoter

AtU3d

Available Since

Aug. 10, 2015

Availability

Academic Institutions and Nonprofits only

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pYJA5

Plasmid

#217778

Purpose

The empty vector for quadruple sgRNA cloning

Depositor

Adriano Aguzzi

Article

Yin et al Nat Biomed Eng. 2024 Dec 4. doi: 10.1038

Type

Empty backbone

Use

CRISPR, Lentiviral, and Synthetic Biology

Tags

Puromycin resistance gene, T2A, and TagBFP

Expression

Mammalian

Promoter

PGK, CMV, U6

Available Since

March 11, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-hNox4

Plasmid

#69352

Purpose

expresses human Nox4 in mammalian cells

Depositor

Karl-Heinz Krause

Article

Serrander et al Biochem J. 2007 Aug 15;406(1):105-14.

Insert

hNOX4 (NOX4 Human)

Expression

Mammalian

Promoter

CMV

Available Since

March 15, 2016

Availability

Academic Institutions and Nonprofits only

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pLenti6/V5-p53_wt p53

Plasmid

#22945

Purpose

3rd generation lentiviral vector encoding human p53 with a C-terminal V5 tag

Depositor

Bernard Futscher

Article

Junk et al Neoplasia. 2008 May . 10(5):450-61.

Insert

tumor protein 53 (TP53 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Available Since

Jan. 27, 2010

Availability

Academic Institutions and Nonprofits only

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pDRM210 LGP (Luciferase GFP Puro)

Plasmid

#174722

Purpose

Expresses the firefly luciferase gene, E2A, eGFP, F2A, and the puromycin resistance gene

Depositor

Wayne Tilley

Article

Mustafa et al Oncogene. 2023 Nov 24. doi: 10.1038/s413

Inserts

Firefly Luciferase
eGFP
pac

Use

Lentiviral and Luciferase

Tags

E2A linker and F2A linker

Expression

Mammalian

Promoter

MND, MND (off Luc-E2A), and MND (off Luc-E2A-eGFP…

Available Since

Nov. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pSpCas9_T2A_mCherry sgAAVS1

Plasmid

#192480

Purpose

All-in-one plasmid for cutting non-targeting locus in DSB Spectrum V1 or V2 cells as control

Depositor

Michael Yaffe

Article

van de Kooij et al Nat Commun. 2022 Sep 8;13(1):5295. doi:

Insert

SpCas9

Tags

T2A mCherry

Expression

Mammalian

Available Since

Dec. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pH2rU3_ForInd_Omicron_sinobiological_Spiked21_T7_CMV_ZsGT2APurR

Plasmid

#204146

Purpose

Lentivirus backbone with BA.1 spike under an inducible promoter and constitutive expressed zsGreen-T2A-PuR genes

Depositor

Jesse Bloom

Article

Dadonaite et al Cell. 2023 Mar 16;186(6):1263-1278.e20.

Inserts

SARS-CoV-2 BA.1 variant spike
zsGreen-T2A-PuR

Use

Lentiviral

Expression

Mammalian

Mutation

21 amino acid cytoplasmic tail deletion

Promoter

CMV and TRE3G

Available Since

Aug. 16, 2023

Availability

Academic Institutions and Nonprofits only

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pLIC-EcTOP

Plasmid

#224463

Purpose

Expression of recombinant Escherichia coli topoisomerase I

Depositor

Yuk-Ching Tse-Dinh

Article

Tan et al Nucleic Acids Res. 2015 Dec 15;43(22):11

Insert

Escherichia coli TopA

Tags

6xHis tag

Expression

Bacterial

Promoter

T7

Available Since

Oct. 31, 2024

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPRv2_mSTING_gRNA_2

Plasmid

#196626

Purpose

Knock-out STING in murine cells, gRNA 2.

Depositor

Nicolas Manel

Article

Jneid et al Sci Immunol. 2023 Jan 13;8(79):eabn6612.

Insert

mSTING gRNA 2 (Sting1 Mouse)

Use

CRISPR and Lentiviral

Mutation

WT

Available Since

Feb. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pLentiRNACRISPR_010-TetO-NLS-RfxCas13d-2A-mCherry-NLS-WPRE-EFS-rtTA3-2A-Blast

Plasmid

#228555

Purpose

Expresses RfxCas13d in mammalian cells for knockdown of target RNAs in combination with compatible crRNAs. Localized to the nucleus

Depositor

Neville Sanjana

Article

Hart et al Nat Biotechnol. 2025 Feb 11. doi: 10.103

Insert

RfxCas13d

Use

Lentiviral

Tags

NLS-HA and P2A-mCherry

Available Since

Aug. 19, 2025

Availability

Academic Institutions and Nonprofits only

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Str-KDEL_CD63-SBP-mCherry

Plasmid

#222326

Purpose

Synchronize the trafficking of CD63 from the ER.

Depositor

Franck Perez

Article

Mathieu et al Nat Commun. 2021 Jul 19;12(1):4389. doi:

Insert

Streptavidin-KDEL and CD63 fused to SBP-mCherry (CD63 Human)

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pCDB327

Plasmid

#113671

Purpose

expresses Ulp1_R3 in Escherichia coli

Depositor

Christopher Bahl

Article

Lau et al J Biol Chem. 2018 Aug 24;293(34):13224-1

Insert

Ulp1_R3

Tags

6-His

Expression

Bacterial

Mutation

contains solubility enhancing mutations

Promoter

T7

Available Since

Sept. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pREDTKI

Plasmid

#51628

Purpose

E coli Genetic Modification

Depositor

Sheng Yang

Article

Yang et al Appl Environ Microbiol. 2014 Jul 1;80(13

Insert

I-SceI (SCEI Budding Yeast)

Use

Red recombinase and i-scei endonuclease

Expression

Bacterial

Promoter

trc

Available Since

May 22, 2015

Availability

Academic Institutions and Nonprofits only

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pHairyRed

Plasmid

#69709

Purpose

Fluorescent reporter of Agrobacterium rhizogenes mediated transformed roots

Depositor

Brett Ferguson, Peter Gresshoff

Article

Lin et al Mol Plant. 2011 May;4(3):537-45. doi: 10

Type

Empty backbone

Expression

Plant

Available Since

Oct. 19, 2015

Availability

Academic Institutions and Nonprofits only

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PH-PLCD1-GFP

Plasmid

#51407

Purpose

Is a biosensor for PI(4,5)P2

Depositor

Tamas Balla

Article

Varnai et al J Cell Biol. 1998 Oct 19;143(2):501-10.

Insert

PH-PLCD1 (aa 1–170) (PLCD1 Human)

Tags

EGFP

Expression

Mammalian

Available Since

March 31, 2014

Availability

Academic Institutions and Nonprofits only

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Arl13b-EGFP

Plasmid

#234844

Purpose

A mammalian expression plasmid encoding Arl13b with EGFP at C-terminus.

Depositor

Lei Lu

Article

Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393

Insert

Arl13b (ARL13B Human)

Expression

Mammalian

Available Since

June 26, 2025

Availability

Academic Institutions and Nonprofits only

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2xmyc-RAB10

Plasmid

#164631

Purpose

Expresses RAB10 in mammalian cells with a 2xmyc tag

Depositor

Mark Cookson

Article

Bonet-Ponce et al Sci Adv. 2020 Nov 11;6(46). pii: 6/46/ea

Insert

Ras-related protein Rab-10 (RAB10 Human)

Tags

2xmyc

Expression

Mammalian

Promoter

CMV

Available Since

July 23, 2021

Availability

Academic Institutions and Nonprofits only

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pNP1950

Plasmid

#222729

Purpose

J23119-BsaI_bRNA Golden Gate Landing Pad-HDV; T7-IS621 Recombinase

Depositor

Patrick Hsu

Article

Durrant et al Nature. 2024 Jun;630(8018):984-993. doi:

Type

Empty backbone

Expression

Bacterial

Available Since

July 24, 2024

Availability

Academic Institutions and Nonprofits only

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