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pCS2+hSpCas9
(Plasmid #51815)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 51815 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pCS2
  • Backbone size w/o insert (bp) 4071
  • Total vector size (bp) 8343
  • Modifications to backbone
    Cloned in hSpCas9 expression sequence
  • Vector type
    Mammalian Expression, CRISPR ; medaka, zebrafish

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    humanized S.pyogenes Cas9
  • Alt name
    SpCas9
  • Alt name
    hSpCas9
  • Insert Size (bp)
    4272
  • Promoter SP6
  • Tag / Fusion Protein
    • 3xFLAG (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site Xba I (not destroyed)
  • 5′ sequencing primer SP6
  • 3′ sequencing primer T3
  • (Common Sequencing Primers)

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    Dr. Zhang produced the hSpCas9 gene, which has been deposited to Addgene (Plasmid 42230: pX330-U6-Chimeric_BB-CBh-hSpCas9 | Cong et al. Science. 3 Jan 2013).
  • Terms and Licenses
  • Articles Citing this Plasmid

Depositor Comments

This plasmid is modified from pX330-U6-Chimeric BB-CBh-hSpCas9 originated from Feng Zhang Lab.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCS2+hSpCas9 was a gift from Masato Kinoshita (Addgene plasmid # 51815 ; http://n2t.net/addgene:51815 ; RRID:Addgene_51815)
  • For your References section:

    Targeted mutagenesis using CRISPR/Cas system in medaka. Ansai S, Kinoshita M. Biol Open. 2014 Apr 11;3(5):362-71. doi: 10.1242/bio.20148177. 10.1242/bio.20148177 PubMed 24728957