Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Addgene is open for ordering and depositing; find up-to-date details here. To learn more about how we are supporting COVID-19 research and to find related plasmids, check out our COVID-19 and Coronavirus Plasmids & Resources page.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected] Learn more

pCS2+hSpCas9 Citations (18)

Plasmid Article: Targeted mutagenesis using CRISPR/Cas system in medaka.
Ansai S, Kinoshita M Biol Open. 2014 Apr 11;3(5):362-71. doi: 10.1242/bio.20148177.
PubMed Journal

Articles Citing pCS2+hSpCas9

Articles
ADAM12-deficient zebrafish exhibit retardation in body growth at the juvenile stage without developmental defects. Tokumasu Y, Iida A, Wang Z, Ansai S, Kinoshita M, Sehara-Fujisawa A. Dev Growth Differ. 2016 May;58(4):409-21. doi: 10.1111/dgd.12286. Epub 2016 May 16. PubMed
Targeted candidate gene screens using CRISPR/Cas9 technology. Shah AN, Moens CB, Miller AC. Methods Cell Biol. 2016;135:89-106. doi: 10.1016/bs.mcb.2016.01.008. Epub 2016 Feb 26. PubMed
Detection of Multiple Genome Modifications Induced by the CRISPR/Cas9 System. Ota S, Kawahara A. Methods Mol Biol. 2016;1451:53-63. doi: 10.1007/978-1-4939-3771-4_4. PubMed
CRISPR/Cas9-Mediated Targeted Knockin of Exogenous Reporter Genes in Zebrafish. Kawahara A. Methods Mol Biol. 2017;1630:165-173. doi: 10.1007/978-1-4939-7128-2_14. PubMed
Genome Editing of Medaka. Ansai S, Kinoshita M. Methods Mol Biol. 2017;1630:175-188. doi: 10.1007/978-1-4939-7128-2_15. PubMed
Biochemical characterization of a medaka (Oryzias latipes) orthologue for mammalian Factor XIII and establishment of a gene-edited mutant. Horimizu R, Ogawa R, Watanabe Y, Tatsukawa H, Kinoshita M, Hashimoto H, Hitomi K. FEBS J. 2017 Jun 27. doi: 10.1111/febs.14153. PubMed
An efficient system for homology-dependent targeted gene integration in medaka (Oryzias latipes). Murakami Y, Ansai S, Yonemura A, Kinoshita M. Zoological Lett. 2017 Jul 6;3:10. doi: 10.1186/s40851-017-0071-x. eCollection 2017. PubMed
Using CRISPR/Cas9-mediated gene editing to further explore growth and trade-off effects in myostatin-mutated F4 medaka (Oryzias latipes). Yeh YC, Kinoshita M, Ng TH, Chang YH, Maekawa S, Chiang YA, Aoki T, Wang HC. Sci Rep. 2017 Sep 12;7(1):11435. doi: 10.1038/s41598-017-09966-9. PubMed
Higher susceptibility to osmolality of the medaka (Oryzias latipes) mutants in orthologue genes of mammalian skin transglutaminases. Watanabe Y, Furukawa E, Tatsukawa H, Hashimoto H, Kamei Y, Taniguchi Y, Hitomi K. Biosci Biotechnol Biochem. 2018 Jul;82(7):1165-1168. doi: 10.1080/09168451.2018.1453294. Epub 2018 Mar 23. PubMed
Distinct interactions of Sox5 and Sox10 in fate specification of pigment cells in medaka and zebrafish. Nagao Y, Takada H, Miyadai M, Adachi T, Seki R, Kamei Y, Hara I, Taniguchi Y, Naruse K, Hibi M, Kelsh RN, Hashimoto H. PLoS Genet. 2018 Apr 5;14(4):e1007260. doi: 10.1371/journal.pgen.1007260. eCollection 2018 Apr. PubMed
Protocadherin-Mediated Cell Repulsion Controls the Central Topography and Efferent Projections of the Abducens Nucleus. Asakawa K, Kawakami K. Cell Rep. 2018 Aug 7;24(6):1562-1572. doi: 10.1016/j.celrep.2018.07.024. PubMed
Genome editing of pufferfish saxitoxin- and tetrodotoxin-binding protein type 2 in Takifugu rubripes. Kato-Unoki Y, Takai Y, Kinoshita M, Mochizuki T, Tatsuno R, Shimasaki Y, Oshima Y. Toxicon. 2018 Oct;153:58-61. doi: 10.1016/j.toxicon.2018.08.001. Epub 2018 Aug 29. PubMed
A tRNA-based multiplex sgRNA expression system in zebrafish and its application to generation of transgenic albino fish. Shiraki T, Kawakami K. Sci Rep. 2018 Sep 6;8(1):13366. doi: 10.1038/s41598-018-31476-5. PubMed
Functional Studies of Transcriptional Cofactors via Microinjection-Mediated Gene Editing in Xenopus. Shibata Y, Bao L, Fu L, Shi B, Shi YB. Methods Mol Biol. 2019;1874:507-524. doi: 10.1007/978-1-4939-8831-0_29. PubMed
Nuclear Progestin Receptor Phosphorylation by Cdk9 Is Required for the Expression of Mmp15, a Protease Indispensable for Ovulation in Medaka. Ogiwara K, Takahashi T. Cells. 2019 Mar 4;8(3). pii: cells8030215. doi: 10.3390/cells8030215. PubMed
Optogenetic modulation of TDP-43 oligomerization accelerates ALS-related pathologies in the spinal motor neurons. Asakawa K, Handa H, Kawakami K. Nat Commun. 2020 Feb 21;11(1):1004. doi: 10.1038/s41467-020-14815-x. PubMed
Chondroitin sulfate proteoglycan 4 regulates zebrafish body axis organization via Wnt/planar cell polarity pathway. Lee YH, Kawakami K, HuangFu WC, Liu IH. PLoS One. 2020 Apr 2;15(4):e0230943. doi: 10.1371/journal.pone.0230943. eCollection 2020. PubMed
Gene disruption of medaka (Oryzias latipes) orthologue for mammalian tissue-type transglutaminase (TG2) causes movement retardation. Watanabe Y, Okuya K, Takada Y, Kinoshita M, Yokoi S, Chisada S, Kamei Y, Tatsukawa H, Yamamoto N, Abe H, Hashimoto H, Hitomi K. J Biochem. 2020 Apr 6. pii: 5816600. doi: 10.1093/jb/mvaa038. PubMed

If you have published an article using this plasmid, please email us at [email protected] to have your article added to this page.