163,606 results

1. pET259-pUC57 24xMS2V6

   Plasmid

   #104391

   Purpose

   MS2V6 cloning

   Depositor

   Evelina Tutucci, Robert Singer

   Article

   Tutucci et al Nat Methods. 2017 Nov 13. doi: 10.1038/n

   Insert

   24xMS2V6
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   All Loops are U-variants, interspaced by 50 nucle…

   Promoter

   Available Since

   Jan. 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. PB-U6insert-EF1puro

   Plasmid

   #104537

   Purpose

   A piggybac vector with puromycin resistance and a U6 promoter that allows cloning of guide RNAs

   Depositor

   George Church

   Article

   Kalhor et al Science. 2018 Aug 9. pii: science.aat980

   Type

   Empty backbone

   Use

   CRISPR, Mouse Targeting, and Synthetic Biology ; …

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available Since

   March 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. mNG2(1-10)_tdTomato

   Plasmid

   #184169

   Purpose

   Lentiviral vector for expression and selection of the mNG2(1-10) split fluorescent protein.

   Depositor

   Ophir Shalem

   Article

   Serebrenik et al Genome Res. 2019 Aug;29(8):1322-1328. do

   Inserts

   mNG2(1-10)
   tdTomato
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   May 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA-miRFP670nano3

   Plasmid

   #184664

   Purpose

   Monomeric near-infrared fluorescent protein miRFP670nano3 in mammalian plasmid

   Depositor

   Vladislav Verkhusha

   Article

   Oliinyk et al Nat Methods. 2022 May 23. pii: 10.1038/s

   Insert

   miRFP670nano3
   

   Use

   Tags

   His

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   June 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pIRES2-EGFP Mer

   Plasmid

   #14998

   Depositor

   Raymond Birge

   Article

   Wu et al J Cell Sci. 2005 Feb 1. 118(Pt 3):539-53

   Insert

   Mer (Mertk Mouse)
   

   Use

   Tags

   IRES EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Aug. 22, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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6. p201N Cas9

   Plasmid

   #59175

   Purpose

   Cas9 driven by double 35S, nptII for plant selection, I-PpoI site to accept gRNA from pUC gRNA Shuttle

   Depositor

   Wayne Parrott

   Article

   Jacobs et al BMC Biotechnol. 2015 Mar 12;15:16. doi:

   Inserts

   Cas9
   nptII
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   2x35S and StUbi-3P

   Available Since

   Sept. 26, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. pRDA_550

   Plasmid

   #203398

   Purpose

   All-in-one lentiviral expression of gRNA and EnAsCas12a

   Depositor

   John Doench

   Article

   Esmaeili Anvar et al Nat Commun. 2024 Apr 27;15(1):3577. doi:

   Insert

   Enhanced Acidaminococcus Cas12a
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   EF1a

   Available Since

   Aug. 31, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCDF-1b-RFC140ΔN555

   Plasmid

   #175043

   Purpose

   Overexpression of human (Rfc1) p140ΔN555 in E.coli

   Depositor

   Brian Kelch

   Article

   Gaubitz et al Proc Natl Acad Sci U S A. 2020 Sep 22;11

   Insert

   Replication factor C subunit 1 (RFC1 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7 promoter

   Available Since

   Jan. 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pRK5-FLAG-SZT2

   Plasmid

   #87034

   Purpose

   mammalian expression of SZT2

   Depositor

   David Sabatini

   Article

   Wolfson et al Nature. 2017 Feb 15. doi: 10.1038/nature

   Insert

   SZT2 (SZT2 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   March 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA-HA-deLACCO1-NGR

    Plasmid

    #208024

    Purpose

    Control biosensor for extracellular L-lactate

    Depositor

    Robert Campbell

    Article

    Nasu et al Nat Commun. 2023 Oct 27;14(1):6598. doi:

    Insert

    deLACCO1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pGL4.23-5xE-box

    Plasmid

    #206841

    Purpose

    The plasmid contains a 312 bp DNA fragment that contained 5 canonical E-boxes (GCCACGTGCA) spaced by 50 nucleotides and cloned into pGL4.23[luc2/minP] (XhoI/HindIII).

    Depositor

    Tassula Proikas-Cezanne

    Article

    Sporbeck et al Commun Biol. 2023 Aug 24;6(1):872. doi:

    Insert

    5x E-box ((GCCACGTGCA)
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Jan. 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pME pHluorin2

    Plasmid

    #73794

    Purpose

    ratiometric pHluorin2 sequence in a middle entry clone

    Depositor

    David Raible

    Article

    Stawicki et al Dis Model Mech. 2014 Jul;7(7):847-56. do

    Insert

    ratiometric pHluorin2
    

    Use

    Middle entry clone

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Feb. 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET24a isPETase

    Plasmid

    #225759

    Purpose

    Expresses codon-optimized PETase from Ideonella sakaiensis in E. coli without native signal sequence and with a C-terminal his-tag

    Depositor

    Jason Boock

    Article

    Carter et al Microb Cell Fact. 2024 Nov 25;23(1):319.

    Insert

    isPETase
    

    Use

    Tags

    6xHis Tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Dec. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAcBac2.tR4-OMeYRS/GFP*

    Plasmid

    #50831

    Purpose

    baculovirus-based delivery system that enables the efficient incorporation of unnatural amino acids into proteins in mammalian cells

    Depositor

    Peter Schultz

    Article

    Chatterjee et al Proc Natl Acad Sci U S A. 2013 Jul 16;11

    Inserts

    two-copy Tyr tRNA cassette
    EGFP*
    OMeYRS
    two copy Tyr tRNA cassette
    

    Use

    Baculoviral

    Tags

    His, Myc-6xHis, and WPRE

    Expression

    Insect

    Mutation

    Y39TAG and able to charge various unnatural amino…

    Promoter

    CAG, CMV, and U6 and H1

    Available Since

    June 3, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pHR-UCOE-SFFV-dCas9-mCherry-ZIM3-KRAB

    Plasmid

    #154473

    Purpose

    Expresses dCas9 fused to mCherry and the KRAB domain of ZIM3

    Depositor

    Mikko Taipale

    Article

    Alerasool et al Nat Methods. 2020 Nov;17(11):1093-1096.

    Insert

    ZIM3 (ZIM3 Human)
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Includes ZIM3 aa 1-100

    Promoter

    SFFV

    Available Since

    Oct. 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pClneoMyc-LATS2

    Plasmid

    #66852

    Purpose

    Express Myc-tagged LATS2 in mammalian cells

    Depositor

    Yutaka Hata

    Article

    Bao et al J Biochem. 2011 Aug;150(2):199-208. doi:

    Insert

    LATS2 (LATS2 Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Aug. 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. MAC-TFRC

    Plasmid

    #172420

    Purpose

    MAC-tagged gene expression

    Depositor

    Markku Varjosalo

    Article

    Liu et al Mol Syst Biol. 2021 Nov;17(11):e10396. d

    Insert

    Transferrin receptor protein 1 (TfR) (CD antigen CD71) (TFRC Human)
    

    Use

    Tags

    MAC-tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV promoter

    Available Since

    Nov. 4, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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18. pF146 pSOD1WTAcGFP1

    Plasmid

    #26407

    Depositor

    Elizabeth Fisher

    Article

    Stevens et al PLoS One. 2010 . 5(3):e9541.

    Insert

    SOD1 (SOD1 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 8, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. FUdeltaGW-rtTA

    Plasmid

    #19780

    Purpose

    3rd generation lentiviral vector

    Depositor

    Konrad Hochedlinger

    Article

    Maherali et al Cell Stem Cell. 2008 Sep 11. 3(3):340-5.

    Insert

    reverse tetracycline transactivator
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Feb. 24, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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20. mBaoJin-lifeactin

    Plasmid

    #229608

    Purpose

    Expresses mBaoJin-lifeactin in mammalian cells

    Depositor

    Kiryl Piatkevich

    Article

    Zhang et al Nat Methods. 2024 Apr;21(4):657-665. doi

    Insert

    mBaoJin
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge