165,971 results

1. PET-HiFi SpCas9-NLS-6xHis

   Plasmid

   #207376

   Purpose

   Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

   Insert

   HiFi SpCas9
   

   Use

   CRISPR

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   R691A

   Promoter

   T7

   Available Since

   Sept. 25, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pX459-sgAAVS1

   Plasmid

   #184403

   Purpose

   pX459 (sgRNA and Cas9 expressing plasmid, RRID:Addgene_62988) with targeting sequence specific to AAVS1; targeting sequence is: GGGGCCACTAGGGACAGGAT

   Depositor

   Robert Bradley

   Article

   Udy et al Life Sci Alliance. 2021 Dec 8;5(3). pii:

   Insert

   AAVS1-specific sgRNA
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6 promoter

   Available Since

   May 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMT646 human L1 ORF2p-3xFlag (ORFeus-Hs, CMV promoter) in pCEP4 Puro

   Plasmid

   #213026

   Purpose

   Expresses full length human LINE-1 in human cells (codon optimized ORFeus-Hs), with a C-terminal 3xFlag tag on ORF2

   Depositor

   Martin S. Taylor

   Article

   Baldwin et al Nature. 2024 Feb;626(7997):194-206. doi:

   Insert

   Human LINE-1 (ORFeus-Hs codon optimized sequence) with ORF2-3xFlag
   

   Tags

   3xFlag (ORF2p)

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV.CAG.iGluSnFR3.v857.PDGFR

   Plasmid

   #178333

   Purpose

   Fluorescent reporter for glutamate, third generation, variant 857. iGluSnFR3.v857

   Depositor

   Kaspar Podgorski

   Article

   Aggarwal et al Nat Methods. 2023 May 4. doi: 10.1038/s4

   Insert

   pAAV CAG iGluSnFR3 v857.PDGFR
   

   Use

   AAV, Cre/Lox, Mouse Targeting, and Synthetic Biol…

   Tags

   IgK-chain, Myc epi tag, and PDGFR TM Domain

   Expression

   Mammalian

   Available Since

   Feb. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGL-p21UTR

   Plasmid

   #20014

   Depositor

   Robert Blelloch

   Article

   Wang et al Nat Genet. 2008 Dec . 40(12):1478-83.

   Insert

   p21 3'UTR (Cdkn1a Mouse)
   

   Use

   Luciferase

   Expression

   Mammalian

   Available Since

   Aug. 5, 2009

   Availability

   Academic Institutions and Nonprofits only

6. H2B-mNeonGreen-IRESpuro2

   Plasmid

   #183745

   Purpose

   Expresses H2B-mNeonGreen

   Depositor

   Daniel Gerlich

   Article

   Cuylen et al Nature. 2016 Jun 29;535(7611):308-12. do

   Insert

   H2B (H2BC16P Human)
   

   Tags

   mNeonGreen

   Expression

   Bacterial and Mammalian

   Promoter

   CMV

   Available Since

   July 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. Human J-chain

   Plasmid

   #158215

   Purpose

   Express the human J-chain

   Depositor

   Junyu Xiao

   Article

   Li et al Science. 2020 Feb 28;367(6481):1014-1017

   Insert

   JCHAIN (JCHAIN Human)
   

   Tags

   8×his

   Expression

   Mammalian

   Available Since

   Oct. 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLX307-VHL(C162F)-3F-BirA

   Plasmid

   #220149

   Purpose

   Expresses VHL(C162F)-BirA as a control for VHL substrate identification by E-STUB

   Depositor

   William Sellers

   Article

   Huang et al Nat Chem Biol. 2024 Mar 21. doi: 10.1038

   Insert

   von Hippel-Lindau tumor suppressor (VHL Human)
   

   Use

   Lentiviral

   Tags

   3F-BirA

   Expression

   Mammalian

   Mutation

   changed cysteine 162 to phenylalanine; codon opti…

   Promoter

   EF-1a

   Available Since

   May 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pOSIP-KL-mcherry

   Plasmid

   #116947

   Purpose

   Integrating mCherry into lambda attB in E. coli chromosome.

   Depositor

   David Bikard

   Article

   Cui et al Nat Commun. 2018 May 15;9(1):1912. doi:

   Insert

   mcherry
   

   Available Since

   March 11, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. HA-p62

    Plasmid

    #28027

    Purpose

    Mammalian expression of HA tagged human p62

    Depositor

    Qing Zhong

    Article

    Fan et al Autophagy. 2010 Jul 22. 6(5):.

    Insert

    p62 (SQSTM1 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Available Since

    Feb. 17, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAR028 Lenti pEF-dEnAsCas12a-NLS-ZNF705F KRAB-3XHA-T2A-BSD

    Plasmid

    #195545

    Purpose

    dCas12a effector fusion for manipulating transcription

    Depositor

    Michael Bassik

    Article

    Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

    Insert

    Lenti pEF-dEnAsCas12a-NLS-TopKRAB-3XHA-T2A-BSD
    

    Use

    CRISPR and Lentiviral

    Tags

    3XHA

    Expression

    Mammalian

    Promoter

    pEF1a

    Available Since

    May 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. cytoplasmic NAD+ biosensor

    Plasmid

    #186787

    Purpose

    NAD+ biosensor comprised of a circularly permuted Venus fluorescent protein (cpVenus), a bipartite NAD+-binding domain modeled from bacterial DNA ligase, and a localization tag to target the cytoplasm

    Depositor

    Michael Cohen

    Article

    Cambronne et al Science. 2016 Jun 17;352(6292):1474-7. d

    Insert

    cytoplasmic NAD+ biosensor
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    July 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3.1-MS2-HA-HsRoquin1_AE

    Plasmid

    #148611

    Purpose

    Mammalian Expression of HsRoquin1

    Depositor

    Elisa Izaurralde

    Article

    Sgromo et al Nat Commun. 2017 Feb 6;8:14307. doi: 10.

    Insert

    HsRoquin1 (RC3H1 Human)
    

    Expression

    Mammalian

    Available Since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. SNAP-Rab5

    Plasmid

    #169069

    Purpose

    Express human Rab5b in mammalian cells

    Depositor

    Gia Voeltz

    Article

    Wu et al Dev Cell. 2021 Jan 11;56(1):52-66.e7. do

    Insert

    Rab5B (RAB5B Human)
    

    Tags

    SNAP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. mEGFP-NbALFA

    Plasmid

    #159986

    Purpose

    For the expresson and purification of the EGFP-tagged anti-ALFA tag nanobody (NbALFA)

    Depositor

    Motoyuki Hattori

    Article

    Jin et al Commun Biol. 2021 Mar 19;4(1):366. doi:

    Insert

    mEGFP-NbALFA
    

    Tags

    8histidine-HRV3C protease cleavage site

    Expression

    Bacterial

    Mutation

    3GS linker between mEGFP and NbALFA

    Available Since

    Oct. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV-mCherry-flex-dtA

    Plasmid

    #58536

    Purpose

    Expresses diphtheria toxin A subunit in Cre positive cells, and mCherry in all the infected cells

    Depositor

    Naoshige Uchida

    Article

    Wu et al Nature. 2014 May 15;509(7500):325-30. do

    Insert

    dtA (DIP_RS18250 )
    

    Use

    AAV and Cre/Lox

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Aug. 22, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. pJYScr

    Plasmid

    #132719

    Purpose

    Plasmid for an easy-to-construct crRNA delivery vector using hybridized oligonucleotides. Constitutive transcription of crRNA, Spectinomycin resistance

    Depositor

    Jan Marienhagen

    Article

    Krumbach et al ACS Synth Biol. 2019 Dec 11. doi: 10.102

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Bacterial

    Available Since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. mPiezo2-CMV-Sport6

    Plasmid

    #81073

    Purpose

    mouse Piezo2

    Depositor

    Ardem Patapoutian

    Article

    Coste et al Nat Commun. 2015 May 26;6:7223. doi: 10.

    Insert

    Piezo2 (please see depositor comments below) (Piezo2 Mouse)
    

    Expression

    Mammalian

    Available Since

    Nov. 18, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. qTAG-AAVS1-Ef1a-Blast

    Plasmid

    #227267

    Purpose

    Empty AAVS1 targeting donor for the insertion of Blast and a strong EF1a promoter. A CDS can be cloned adjacent to the promoter via restriction or gibson cloning using the MluI cut site.

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Type

    Empty backbone

    Use

    CRISPR; Donor cassette

    Expression

    Mammalian

    Available Since

    Nov. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pet21_TurboID_18A

    Plasmid

    #170922

    Purpose

    Express TurboID_18A in Bl21

    Depositor

    Huan Bao

    Article

    Bao et al Commun Biol. 2021 Apr 30;4(1):514. doi:

    Insert

    TurboID-18a
    

    Expression

    Bacterial

    Available Since

    July 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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