165,971 results

1. pCDNA3 Flag Jnk2a2

   Plasmid

   #13755

   Depositor

   Roger Davis

   Article

   Gupta et al EMBO J. 1996 Jun 3. 15(11):2760-70.

   Insert

   Jnk2a2 (MAPK9 Human)
   

   Tags

   Flag

   Expression

   Mammalian

   Available Since

   Feb. 22, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV.CAG.(cyto).iATPSnFR2.S29W.A95K.HaloTag

   Plasmid

   #209651

   Purpose

   Expression of iATPSnFR2, high affinity

   Depositor

   Tim Brown, HHMI-JRC Tool Translation Team

   Article

   Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

   Insert

   iATPSnFR2.S29W.HaloTag
   

   Use

   AAV

   Mutation

   S29W.A95K

   Promoter

   CAG

   Available Since

   June 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. SpRYc

   Plasmid

   #175575

   Purpose

   Expresses the codon-optimized, engineered SpRYc Cas9 nuclease in mammalian cells

   Depositor

   Joseph Jacobson

   Article

   Koseki et al Res Sq. 2023 Mar 7:rs.3.rs-2625838. doi:

   Insert

   SpRYc
   

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   PID of SpRY grafted onto the N-terminal domain of…

   Promoter

   CMV

   Available Since

   May 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMT85_SpdCas9_pmcDA1

   Plasmid

   #204614

   Purpose

   CRISPR cytosine deaminase integrative plasmid for Mycoplasma bovis

   Depositor

   Pascal Sirand-Pugnet

   Article

   Ipoutcha et al Appl Environ Microbiol. 2022 Sep 13;88(1

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Bacterial

   Available Since

   Aug. 25, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pRK5F Smad3

   Plasmid

   #12625

   Depositor

   Rik Derynck

   Article

   Zhang et al Nature. 1998 Aug 27. 394(6696):909-13.

   Insert

   Smad3 (SMAD3 Human)
   

   Tags

   Flag

   Expression

   Mammalian

   Available Since

   Oct. 10, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET MBP GFP LIC cloning vector (MBP-msfGFP)

   Plasmid

   #29750

   Depositor

   Scott Gradia

   Type

   Empty backbone

   Tags

   6xHis, AviTag (biotin), MBP, SiriusGFP, and TEV c…

   Expression

   Bacterial

   Available Since

   Aug. 17, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAR013 Lenti pEF-dEnAsCas12a-NLS-VP64-3XHA-T2A-BSD

   Plasmid

   #195547

   Purpose

   dCas12a effector fusion for manipulating transcription

   Depositor

   Michael Bassik

   Article

   Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

   Insert

   Lenti pEF-dEnAsCas12a-NLS-VP64-3XHA-T2A-BSD
   

   Use

   CRISPR and Lentiviral

   Tags

   3XHA

   Expression

   Mammalian

   Promoter

   pEF1a

   Available Since

   May 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pTRIP-SFFV-EGFP-NLS-FLAG- cGAS E225A/D227A

   Plasmid

   #127660

   Purpose

   Over-express catalytically inactive EGFP-NLS-FLAG-cGAS E225A/D227A

   Depositor

   Nicolas Manel

   Article

   Gentili et al Cell Rep. 2019 Feb 26;26(9):2377-2393.e1

   Insert

   cGAS (CGAS Human)
   

   Use

   Lentiviral

   Mutation

   E225A/D227A

   Available Since

   July 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLentiPGK DEST H2B-iRFP670

   Plasmid

   #90237

   Purpose

   Lentiviral vector to express H2B-iRFP670 under PGK promoter

   Depositor

   Markus Covert

   Article

   Kudo et al Nat Protoc. 2018 Jan;13(1):155-169. doi:

   Insert

   H2B
   

   Use

   Lentiviral

   Tags

   iRFP670

   Expression

   Mammalian

   Promoter

   PGK

   Available Since

   March 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. EF1a_HNF4A_P2A_Hygro_Barcode

    Plasmid

    #120450

    Purpose

    Barcoded lentiviral vector to express HNF4A in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

    Depositor

    Prashant Mali

    Article

    Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

    Insert

    HNF4A (HNF4A Human)
    

    Use

    Lentiviral

    Promoter

    EF1a

    Available Since

    Feb. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. Fucci(CA) hCdt1-iRFP hGeminin-TagBFP2

    Plasmid

    #190181

    Purpose

    Fluorescent reporter vector to visualize all of cell cycle phases.

    Depositor

    Toshiro Sato

    Article

    Ohta et al Nature. 2022 Jul 7. pii: 10.1038/s41586-

    Inserts

    iRFP
    hCdt1(1/100)Cy(-) (Cdt1 Synthetic)
    TagBFP2
    hGeminin(1/110)
    

    Expression

    Bacterial and Mammalian

    Promoter

    CMV

    Available Since

    Feb. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. SCTR-Tango

    Plasmid

    #66501

    Purpose

    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

    Depositor

    Bryan Roth

    Article

    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

    Insert

    SCTR (SCTR Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

13. AICSDP-63: ACTN2-mEGFP

    Plasmid

    #124607

    Purpose

    Homology arms and linker-mEGFP sequence for C-terminus tagging of human ACTN2, via excisable Cas9-excisable CAGGS-mCherry selection cassette

    Depositor

    Allen Institute for Cell Science

    Insert

    ACTN2 Homology Arms with linker-mEGFP and Cas9-excisable selection cassette (ACTN2 Human)
    

    Use

    CRISPR; Donor template

    Expression

    Mammalian

    Available Since

    May 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. T5-pAc5.1 EGFP-EcoRV

    Plasmid

    #206481

    Purpose

    Schneider cell imaging

    Depositor

    Anne Ephrussi

    Article

    Salgania et al Nat Commun. 2024 Apr 3;15(1):2875. doi:

    Type

    Empty backbone

    Tags

    EGFP

    Expression

    Insect

    Available Since

    July 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. p006-GFP-pBAD

    Plasmid

    #108315

    Purpose

    GFP from jellyfish Aquorea sp. under pBAD promoter with AraC repressor; kanamycin resistant.

    Depositor

    Jaroslaw Bryk

    Article

    Siddall et al bioRxiv 2021.06.20.449138

    Insert

    GFP under control of arabinose-inducible promoter pBAD
    

    Expression

    Bacterial

    Promoter

    pBAD

    Available Since

    Aug. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. TFORF2314

    Plasmid

    #142029

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    RXRB (RXRB Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    June 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. Lact-C2-GFP

    Plasmid

    #22852

    Depositor

    Sergio Grinstein

    Article

    Yeung et al Science. 2008 Jan 11. 319(5860):210-3.

    Insert

    Lactadherin-C2 Domain (MFGE8 Bovine)
    

    Tags

    GFP

    Expression

    Mammalian

    Available Since

    Jan. 15, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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18. 35S-eGFP-nosT

    Plasmid

    #80127

    Purpose

    Transient expression vector for eGFP in plants

    Depositor

    Yutaka Kodama

    Article

    Fujii et al Plant Biotech. 2015; 32(1): 81-87.

    Type

    Empty backbone

    Tags

    eGFP

    Expression

    Plant

    Promoter

    CaMV35S

    Available Since

    Dec. 2, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pFastBac His6 MBP Asn10 TEV cloning vector with BioBrick PolyPromoter LIC Subcloning (438-C)

    Plasmid

    #55220

    Depositor

    Scott Gradia

    Article

    Gradia et al Methods Enzymol. 2017;592:1-26. doi: 10.

    Type

    Empty backbone

    Tags

    His6 and MBP, Asn10

    Expression

    Insect

    Available Since

    June 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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20. pDD57

    Plasmid

    #119882

    Purpose

    Constitutive expression of GFP+ on pAL5000/pMB1 backbone with kanamycin resistance.

    Depositor

    Tae Seok Moon

    Article

    DeLorenzo et al ACS Synth Biol. 2018 Feb 16;7(2):727-738

    Insert

    pConstitutive_GFP+
    

    Expression

    Bacterial

    Available Since

    Jan. 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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