163,606 results

1. QPM-sgR (pTaU3)

   Plasmid

   #140448

   Purpose

   For sgRNA expression in monocotyledons protoplasts

   Depositor

   Caixia Gao

   Article

   Lin et al Nat Biotechnol. 2020 May;38(5):582-585.

   Insert

   TaU3-sgRNA
   

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   TaU3

   Available Since

   May 5, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET30a-HS-Tn5

   Plasmid

   #127916

   Purpose

   Prokaryotic expression of Hyperstable Tn5 (HS-Tn5) for Illumina library preperation. Developed for the large scale ChIP-Seq and ATAC-Seq experiments for the fruitENCODE and Maize Cistrome projects.

   Depositor

   Silin Zhong

   Article

   Tu et al Nat Commun. 2020 Oct 9;11(1):5089. doi:

   Insert

   Hyperstable Tn5
   

   Use

   Tags

   E coli elongation factor Ts and Mxe intein - Chit…

   Expression

   Bacterial

   Mutation

   E54K, L372P

   Promoter

   T7

   Available Since

   July 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCMV2-SEP-GluA1 (M1)

   Plasmid

   #64942

   Purpose

   mammalian expression of rat GluA1

   Depositor

   Jeremy Henley

   Article

   Blanco-Suarez et al J Biol Chem. 2014 Feb 21;289(8):4644-51.

   Insert

   GluA1 (Gria1 Rat)
   

   Use

   Tags

   SEP (superecliptic pHluorin)

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   May 14, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pmiRFP720-N1

   Plasmid

   #136560

   Purpose

   A cytoplasmic expression of a monomeric phytochrome-based near-infrared fluorescent protein miRFP720

   Depositor

   Vladislav Verkhusha

   Article

   Matlashov et al Nat Commun. 2020 Jan 13;11(1):239. doi:

   Insert

   miRFP720
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Feb. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLexM-SLC3A2

   Plasmid

   #197994

   Purpose

   Expresses SLC3A2 as an N-terminal His tagged construct in mammalian cells

   Depositor

   Simon Newstead

   Article

   Parker et al Nat Commun. 2021 Dec 8;12(1):7147. doi:

   Insert

   SLC3A2 (SLC3A2 Human)
   

   Use

   Tags

   His tag

   Expression

   Mammalian

   Mutation

   Please see depositor comments below

   Promoter

   CMV

   Available Since

   July 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. ABE9

   Plasmid

   #194208

   Purpose

   Mammalian expression of ABE9

   Depositor

   Dali Li

   Article

   Chen et al Nat Chem Biol. 2022 Oct 13. pii: 10.1038

   Insert

   TadA-8e (N108Q/L145T)-nSpCas9
   

   Use

   CRISPR

   Tags

   6*His

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Jan. 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. kiCAP-AAV-MyoAAV4A

   Plasmid

   #224448

   Purpose

   Rep/Cap plasmid for the production of MyoAAV 4A, a muscle-tropic AAV capsid in mice and macaques.

   Depositor

   Pardis Sabeti

   Article

   Tabebordbar et al Cell. 2021 Sep 16;184(19):4919-4938.e22.

   Insert

   AAV9 VP1 modified with 7mer insertion between amino acids 588 and 589
   

   Use

   AAV

   Tags

   Expression

   Mutation

   RGDYNSL insert between amino acids 588 and 589 of…

   Promoter

   p41

   Available Since

   Sept. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3.1_GEM2-Halo-FRB

   Plasmid

   #197056

   Purpose

   Expression of Synechococcus elongatus encapsulin Halo-FRB-fusion in mammalian cells

   Depositor

   Julia Mahamid

   Article

   Fung et al Nat Methods. 2023 Dec;20(12):1900-1908.

   Insert

   Encapsulin
   

   Use

   Tags

   FRB and HaloTag

   Expression

   Mammalian

   Mutation

   Codon-optimised for human cell expression

   Promoter

   Available Since

   Sept. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCAX APLP1

   Plasmid

   #30141

   Depositor

   Dennis Selkoe, Tracy Young-Pearse

   Article

   Young-Pearse et al J Neurosci. 2007 Dec 26. 27(52):14459-69

   Insert

   Amyloid Precursor Like Protein 1 (APLP1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   July 29, 2011

   Availability

   Academic Institutions and Nonprofits only

10. human p53-(1-393)

    Plasmid

    #24859

    Depositor

    Cheryl Arrowsmith

    Article

    Ayed et al Nat Struct Biol. 2001 Sep . 8(9):756-60.

    Insert

    p53 (TP53 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Full length.

    Promoter

    Available Since

    Aug. 5, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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11. pE-FLP

    Plasmid

    #45978

    Purpose

    Expresses yeast Flp driven by the constitutive promoter pE from phage P2

    Depositor

    Drew Endy, Keith Shearwin

    Article

    St-Pierre et al ACS Synth Biol. 2013 Sep 20;2(9):537-41.

    Insert

    flp
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    flp driven by the constitutive promoter pE from p…

    Promoter

    pE (from phage P2)

    Available Since

    July 26, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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12. pT3TS-RfxCas13d-HA

    Plasmid

    #141320

    Purpose

    Plasmid to carry out IVT of RfxCas13d (human codon-optimized)

    Depositor

    Miguel Angel Moreno-Mateos, Ariel Bazzini

    Article

    Kushawah et al Dev Cell. 2020 Sep 28;54(6):805-817.e7.

    Insert

    Rfx-Cas13d
    

    Use

    CRISPR

    Tags

    HA

    Expression

    Mutation

    Promoter

    Available Since

    Aug. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV-hSyn-mScarlet

    Plasmid

    #131001

    Purpose

    AAV vector to drive the expression of mScarlet under the control of human Synapsin promoter

    Depositor

    Karl Deisseroth

    Article

    Marshel et al Science. 2019 Aug 9;365(6453). pii: scie

    Has Service

    AAV1 and AAV8

    Insert

    mScarlet
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    hSyn

    Available Since

    Sept. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. ABE8e(TadA-8e V106W)

    Plasmid

    #138495

    Purpose

    A-to-G base editor

    Depositor

    David Liu

    Article

    Richter et al Nat Biotechnol. 2020 Jul;38(7):883-891.

    Insert

    ecTadA(8e V106W)-nSpCas9
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    TadA-8e V106W

    Promoter

    Available Since

    March 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV-GFP

    Plasmid

    #32395

    Depositor

    John T Gray

    Article

    Gray et al Methods Mol Biol. 2011;807:25-46.

    Insert

    GFP
    

    Use

    AAV; Adeno-associated virus

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Sept. 28, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET30a-AlkB

    Plasmid

    #79050

    Purpose

    Express wild type AlkB protein in E. coli BL21(DE3)

    Depositor

    Tao Pan

    Article

    Zheng et al Nat Methods. 2015 Sep;12(9):835-7. doi:

    Insert

    AlkB (alkB E.coli)
    

    Use

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    A truncated AlkB with deletion of the amino (N)-t…

    Promoter

    T7

    Available Since

    Aug. 3, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. FLX1.8NFATGFPpd2HS4

    Plasmid

    #169096

    Purpose

    Lentiviral vector encoding a short-lived GFP under the transcriptional control of a mutlimerized NFAT gene enhancer to measure T cell activation

    Depositor

    Geoffrey Owens

    Inserts

    NFAT enhancer (from IL-4 promoter)
    Destabilized green fluorescent protein
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    NFAT enhancer + synthetic TATA box

    Available Since

    July 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pDZ Flag USP28

    Plasmid

    #15665

    Depositor

    Martin Eilers

    Article

    Popov et al Nat Cell Biol. 2007 Jul . 9(7):765-74.

    Insert

    USP28 (USP28 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 2, 2007

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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19. pHAGE-ERBB2

    Plasmid

    #116734

    Purpose

    Lentiviral expression of ERBB2

    Depositor

    Kenneth Scott, Gordon Mills

    Article

    Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

    Insert

    ERBB2 (ERBB2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    WT

    Promoter

    Available Since

    March 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAAV.CAG.LSL.EGFP

    Plasmid

    #100047

    Purpose

    AAV mediated Cre-dependent expression of EGFP (Lox-Stop-Lox)

    Depositor

    Hongkui Zeng

    Article

    Oh et al Nature. 2014 Apr 10;508(7495):207-14. do

    Insert

    EGFP
    

    Use

    AAV and Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available Since

    Jan. 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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