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PPRE-pNL1.3

Plasmid

#84394

Purpose

Secreted Luciferase reporter for PPARgamma activity

Depositor

Severine Degrelle, Thierry Fournier

Publication

Degrelle et al PPAR Res. 2017;2017:6139107. doi: 10.115

Insert

PPARG response element (PPARG Human)

Tags

secNluc

Expression

Mammalian

Promoter

PPRE

Available since

Jan. 23, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pDG459

Plasmid

#100901

Purpose

SpCas9 with 2A-Puro and a cloning backbone for 2 custom gRNAs which can be cloned in via a one-step reaction. For generation of double knock-outs and large deletions in a single plasmid system.

Depositor

Paul Thomas

Publication

Adikusuma et al PLoS One. 2017 Dec 6;12(12):e0187236. do

Inserts

humanized CRISPR associated protein 9
U6-gRNA scaffold 1
U6-gRNA scaffold 2

Use

CRISPR and Mouse Targeting

Tags

3xFLAG and T2A-PuroR

Expression

Mammalian

Promoter

CBh and U6

Available since

Dec. 14, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

9 Articles

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pLenti6.2-3xFLAG-V5-ccdB

Plasmid

#87072

Purpose

Lentiviral Gateway-compatible expression vector backbone with a N-terminal 3xFLAG-V5 tag

Depositor

Susan Lindquist, Mikko Taipale

Publication

Taipale et al Cell. 2014 Jul 17;158(2):434-48. doi: 10

Type

Empty backbone

Use

Lentiviral

Tags

3xFLAG-V5

Expression

Mammalian

Available since

March 7, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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PB-TAC-ERP2

Plasmid

#80478

Purpose

All-in-One piggyBac transposon Destination vector for dox-inducible expression of Gateway cloned elements (inducible mCherry and constitutive rtTA and puromycin resistance)

Depositor

Knut Woltjen

Publication

Kim et al Methods Mol Biol. 2016;1357:111-31. doi:

Type

Empty backbone

Use

Piggybac transposon

Expression

Mammalian

Available since

Nov. 28, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

8 Articles

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pMA7CR_2.0

Plasmid

#73950

Purpose

pMA7CR_2.0 contains arabinose inducible λ/RED β-protein and dam, and aTc inducible CRISPR/Cas9 and recX

Depositor

Alex Nielsen

Publication

Ronda et al Sci Rep. 2016 Jan 22;6:19452. doi: 10.10

Inserts

cas9
lambda beta
dam
recX

Expression

Bacterial

Promoter

pAra and pTet

Available since

May 25, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pMF230

Plasmid

#62546

Purpose

Broad host range plasmid for constitutive expression of the eGFP. Developed for imaging Pseudomonas aeruginosa in biofilms.

Depositor

Michael Franklin

Publication

Nivens et al J Bacteriol. 2001 Feb;183(3):1047-57.

Insert

GFP mut2

Use

Broad host range plasmid

Promoter

trc

Available since

April 21, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

10 Articles

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pLL7.0: tgRFPt-SSPB R73Q

Plasmid

#60416

Purpose

Mammalian Expression of tgRFPt-Micro

Depositor

Brian Kuhlman

Publication

Guntas et al Proc Natl Acad Sci U S A. 2015 Jan 6;112

Insert

tgRFPt-SSPB R73Q

Use

Cre/Lox and Lentiviral

Tags

TagRFPt

Expression

Mammalian

Mutation

R73Q, Y11K and A15E

Promoter

CMV

Available since

Feb. 27, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

10 Articles

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pET22b RNase A

Plasmid

#58903

Purpose

Bacterial expression of bovine pancreatic ribonuclease A

Depositor

Ronald Raines

Publication

delCardayre et al Protein Eng. 1995 Mar;8(3):261-73.

Insert

RNase A (RNASE1 Bovine)

Tags

pelB sequence

Expression

Bacterial

Mutation

See Depositor Comments below

Promoter

T7

Available since

Feb. 12, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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mCherry-Rab5a-7

Plasmid

#55126

Purpose

Localization: Ras GTPase, Excitation: 587, Emission: 610

Depositor

Michael Davidson

Insert

Rab5a

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available since

Sept. 17, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

17 Articles

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MAVS-WT

Plasmid

#52135

Purpose

Retroviral vector for expression of human MAVS in mammalian cells

Depositor

Jonathan Kagan

Publication

Dixit et al Cell. 2010 May 14;141(4):668-81. Epub 20

Insert

MAVS (MAVS Human)

Use

Retroviral

Expression

Mammalian

Available since

Aug. 11, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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mEmerald-Clathrin-15

Plasmid

#54040

Purpose

Localization: Clathrin Vesicles, Excitation: 487, Emission: 509

Depositor

Michael Davidson

Publication

Fiolka et al Proc Natl Acad Sci U S A. 2012 Apr 3;109

Insert

Clathrin (CLTB Human)

Tags

mEmerald

Expression

Mammalian

Promoter

CMV

Available since

July 2, 2014

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

5 Articles

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pCDNA4/Full length FVIII

Plasmid

#41036

Depositor

Robert Peters

Publication

Peters et al J Thromb Haemost. 2012 Dec 4. doi: 10.11

Insert

FVIII (F8 Human)

Expression

Mammalian

Promoter

CMV

Available since

Nov. 6, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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β3-integrin-YFP

Plasmid

#26653

Depositor

Jonathan Jones

Publication

Tsuruta et al FASEB J. 2002 Jun . 16(8):866-8.

Insert

β3-integrin (ITGB3 Human)

Tags

YFP

Expression

Mammalian

Available since

Aug. 18, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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Rab5a-pmCherryC1

Plasmid

#27679

Depositor

Christien Merrifield

Publication

Taylor et al PLoS Biol. 2011 Mar . 9(3):e1000604.

Insert

Rab5a (Rab5a Mouse)

Tags

mCherry

Expression

Mammalian

Available since

May 4, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

8 Articles

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pMV306hsp+LuxG13

Plasmid

#26161

Depositor

Brian Robertson, Siouxsie Wiles

Publication

Andreu et al PLoS One. 2010 May 24;5(5):e10777.

Insert

Bacterial luciferase operon + G13 promoter

Expression

Bacterial

Mutation

It contains a gram-positive enhanced translation …

Available since

Feb. 7, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

40 Articles

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PCMV4-3 HA/IkB-alpha(SS32,36AA)

Plasmid

#24143

Depositor

Warner Greene

Publication

Sun et al Mol Cell Biol. 1996 Mar . 16(3):1058-65.

Insert

IkB alpha (NFKBIA Human)

Tags

3xHA

Expression

Mammalian

Mutation

S32A S36A

Available since

March 25, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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Flag-HA-USP48

Plasmid

#22585

Depositor

Wade Harper

Publication

Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

Insert

USP48 (USP48 Human)

Use

Retroviral

Tags

FLAG and HA

Expression

Mammalian

Mutation

None

Available since

Dec. 4, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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PCDNA3 Flag p38 alpha (agf)

Plasmid

#20352

Depositor

Roger Davis

Publication

Enslen et al J Biol Chem. 1998 Jan 16. 273(3):1741-8.

Insert

pDCNA3 Flag p38 alpha (agf) (Mapk14 Mouse)

Tags

Flag

Expression

Mammalian

Mutation

Replace Thr 180 and Tyr 182 with Ala and Phe resp…

Available since

April 14, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

14 Articles

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YFP-FKBP (YF)

Plasmid

#20175

Depositor

Tobias Meyer

Publication

Inoue et al Nat Methods. 2005 Jun . 2(6):415-8.

Insert

YFP-FKBP (YF): (FKBP1A Human)

Expression

Mammalian

Mutation

FKBP was taken out from pC4M-F2E with a flanking …

Available since

March 24, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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pRSETA-PAGFP

Plasmid

#11911

Depositor

Jennifer Lippincott-Schwartz

Publication

Patterson et al Science. 2002 Sep 13. 297(5588):1873-7.

Insert

pRSETA-PAGFP

Expression

Bacterial

Mutation

The PAGFP has four mutations (L64F/T65S/V163A/T20…

Available since

July 3, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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