163,606 results

1. 2XMyc-LRRK2-WT

   Plasmid

   #25361

   Depositor

   Mark Cookson

   Article

   J Biol Chem. 2008 Jun 13;283(24):16906-1

   Insert

   LRRK2 (LRRK2 Human)
   

   Use

   Tags

   2XMyc

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   June 17, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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2. Luciferase-pcDNA3

   Plasmid

   #18964

   Depositor

   William Kaelin

   Article

   Safran et al Proc Natl Acad Sci U S A. 2006 Jan 3. 10

   Insert

   Firefly Luciferase
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Aug. 20, 2008

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. BFP-Sec61 beta

   Plasmid

   #49154

   Purpose

   expression of Sec61 beta fused to BFP, used as a general ER marker

   Depositor

   Gia Voeltz

   Article

   Zurek et al Traffic. 2011 Jan;12(1):28-41. doi: 10.1

   Insert

   Sec61 beta (SEC61B Human)
   

   Use

   Tags

   BFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   May 29, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. H2B-GFP

   Plasmid

   #11680

   Depositor

   Geoff Wahl

   Article

   Kanda et al Curr Biol. 1998 Mar 26. 8(7):377-85.

   Insert

   Human histone H2B (H2BC11 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   May 31, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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5. TFORF3550

   Plasmid

   #145026

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens. This is the control vector for the collection.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   mCherry
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available Since

   Jan. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET24a-ncOGT-FL

   Plasmid

   #190821

   Purpose

   For expression of full-length human ncOGT in E.coli. The ncOGT is 6x His-tagged on its N-terminus and codon-optimized for E.coli.

   Depositor

   Gerald Hart

   Article

   Zhu et al Cell. 2022 Dec 30:S0092-8674(22)01529-X.

   Insert

   O-GlcNAc Transferase (OGT Human)
   

   Use

   Tags

   6x His

   Expression

   Bacterial

   Mutation

   Codon optimized for expression in E.coli

   Promoter

   T7

   Available Since

   Jan. 9, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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7. Halo-Sec31

   Plasmid

   #166895

   Purpose

   mammalian expression of Sec31 tagged with HaloTag

   Depositor

   Jennifer Lippincott-Schwartz

   Article

   Weigel et al Cell. 2021 Apr 29;184(9):2412-2429.e16.

   Insert

   Sec31
   

   Use

   Tags

   HaloTag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   April 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pYTK-DN4

   Plasmid

   #180285

   Purpose

   KanR multi-gene yeast expression vector (GFP-dropout)

   Depositor

   Andreas Milias-Argeitis

   Article

   Novarina et al STAR Protoc. 2022 Jun 10;3(2):101358. do

   Insert

   ConLS' - GFP - ConRE' - KanR - CEN6/ARS4
   

   Use

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   Available Since

   June 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCDH-puro/Flag PD-L1 WT

   Plasmid

   #121446

   Purpose

   expression of huamn PD-L1 wildtype in mammalian cells

   Depositor

   Mien-Chie Hung

   Article

   Li et al Nat Commun. 2016 Aug 30;7:12632. doi: 10

   Insert

   CD274 (CD274 Human)
   

   Use

   Lentiviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   wildtype

   Promoter

   CMV

   Available Since

   Feb. 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pD3sE_SC12

    Plasmid

    #219720

    Purpose

    DV3 E dimer with fusion loop mutation and increased expression

    Depositor

    Brian Kuhlman

    Article

    Phan et al J Biol Chem. 2022 Jul;298(7):102079. doi

    Insert

    DV3 sE SC12
    

    Use

    Tags

    8xHis and human serum albumin (HSA)

    Expression

    Mammalian

    Mutation

    G106D, A257W, T260R, F277W, A278P, G29K, T33V, A3…

    Promoter

    Available Since

    May 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. MSCV-myc-CAR-2A-Thy1.1

    Plasmid

    #127890

    Purpose

    Expresses CART receptor along with tags.

    Depositor

    Anjana Rao

    Article

    Chen et al Nature. 2019 Mar;567(7749):530-534. doi:

    Inserts

    myc,
    CAR
    2A
    Thy1.1
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    May 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. AmCyan-P2A-mCherry

    Plasmid

    #45350

    Depositor

    Ilpo Huhtaniemi

    Article

    Potorac et al J Endocrinol. 2016 Sep 21. pii: JOE-16-0

    Insert

    AmCyan
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    SV40 NLS

    Promoter

    CMV

    Available Since

    Nov. 6, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pDONR223-HK2

    Plasmid

    #23854

    Depositor

    David Root, William Hahn

    Article

    Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

    Insert

    HK2 (HK2 Human)
    

    Use

    Gateway donor vector

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    July 30, 2010

    Availability

    Academic Institutions and Nonprofits only

14. pTn7CdrA-gfp(ASV)C

    Plasmid

    #111617

    Purpose

    Fluorescence-based gauging of the level of the nucleotide secondary messenger cyclic di-GMP in Pseudomonas aeruginosa and related species.

    Depositor

    Tim Tolker-Nielsen

    Article

    Rybtke et al Appl Environ Microbiol. 2012 Aug;78(15):

    Insert

    PcdrA-RBSII-gfp(ASV)-T0-T1
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    June 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pRK172-α-syn-TEV-GFP

    Plasmid

    #166671

    Purpose

    Bacterial expression of alpha synuclein coupled to GFP via a TEV site for uptake studies

    Depositor

    Iris Lindberg

    Article

    Jarvela et al PLoS One. 2021 Jan 26;16(1):e0241161. do

    Insert

    mouse synuclein (from V. Lee) (Snca Mouse)
    

    Use

    Tags

    GFP

    Expression

    Bacterial

    Mutation

    added a TEV cleavage site sequence between synucl…

    Promoter

    CMV

    Available Since

    July 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. GPRC6A-DuET

    Plasmid

    #213299

    Purpose

    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

    Depositor

    Thomas Sakmar

    Article

    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

    Insert

    GPRC6A (GPRC6A Human)
    

    Use

    Tags

    1D4 and FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV, T7

    Available Since

    April 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. GFP-MAPPER

    Plasmid

    #117721

    Purpose

    MAPPER Selectively Labels ER-PM Junctions

    Depositor

    Jen Liou

    Article

    Chang et al Cell Rep. 2013 Nov 14;5(3):813-25. doi:

    Insert

    MAPPER
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pRS305-Leu2-PINO4-PfV-GS-Sapphire

    Plasmid

    #116930

    Purpose

    Yeast integrative vector for the exression of the 40nm GEM under the INO4 promoter

    Depositor

    Liam Holt

    Article

    Delarue et al Cell. 2018 Jul 12;174(2):338-349.e20. do

    Insert

    Pfv
    

    Use

    Tags

    Sapphire

    Expression

    Yeast

    Mutation

    Promoter

    INO4

    Available Since

    Jan. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pHR-CD28-mCherry

    Plasmid

    #223593

    Purpose

    Lentiviral expression of human CD28 with mCherry tag in mammalian cells

    Depositor

    Enfu Hui

    Article

    Zhao et al Immunity. 2019 Dec 17;51(6):1059-1073.e9

    Insert

    CD28 (CD28 Human)
    

    Use

    Lentiviral; mammalian expression

    Tags

    mCherry

    Expression

    Mutation

    Promoter

    Available Since

    Sept. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA3.1-2xFLAG-SREBP-2

    Plasmid

    #26807

    Depositor

    Timothy Osborne

    Article

    Toth et al Mol Cell Biol. 2004 Sep . 24(18):8288-30

    Insert

    Sterol Regulatory Element Binding Protein 2 (SREBF2 Human)
    

    Use

    Tags

    2x FLAG

    Expression

    Mammalian

    Mutation

    L7V, G76S, K395R and I482L

    Promoter

    CMV

    Available Since

    July 26, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge