pcDNA3 deltaN89 Beta-catenin
Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||19288||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 5400
Vector typeMammalian Expression
Selectable markersNeomycin (select with G418)
Growth in Bacteria
Copy numberHigh Copy
SpeciesH. sapiens (human)
Insert Size (bp)2100
MutationN-terminal truncation, deleted aa 1-89
Entrez GeneCTNNB1 (a.k.a. CTNNB, EVR7, MRD19, NEDSDV, armadillo)
/ Fusion Protein
- Flag (C terminal on insert)
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site NotI (not destroyed)
- 5′ sequencing primer CMV-F
- 3′ sequencing primer BGH rev (Common Sequencing Primers)
Has 12-bp sequence upstream of the initiating ATG codon matching the consensus Kozak initiator sequence.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pcDNA3 deltaN89 Beta-catenin was a gift from Eric Fearon (Addgene plasmid # 19288 ; http://n2t.net/addgene:19288 ; RRID:Addgene_19288)
For your References section:Neoplastic transformation of RK3E by mutant beta-catenin requires deregulation of Tcf/Lef transcription but not activation of c-myc expression. Kolligs FT, Hu G, Dang CV, Fearon ER. Mol Cell Biol. 1999 Aug . 19(8):5696-706. PubMed 10409758
Map uploaded by the depositor.