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Tubp-Gal80 in pCaSpeR4
(Plasmid #17748)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 17748 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    GAL80
  • Alt name
    Tubp-GAL80
  • Alt name
    GAL80 SV40 polyA
  • Alt name
    SV40 polyA
  • Species
    S. cerevisiae (budding yeast)
  • Insert Size (bp)
    4700
  • GenBank ID
    X01667
  • Entrez Gene
    GAL80 (a.k.a. YML051W)
  • Tags / Fusion Proteins
    • SV40 polyA (C terminal on insert)
    • Tubulin 1alpha promoter (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site Stu (not destroyed)
  • 5′ sequencing primer n/a
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    GAL80 construct from Jun Ma; tubulin 1alpha promoter fragment from Gary Struhl
  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry
  • Articles Citing this Plasmid
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Tubp-Gal80 in pCaSpeR4 was a gift from Liqun Luo (Addgene plasmid # 17748 ; http://n2t.net/addgene:17748 ; RRID:Addgene_17748)
  • For your References section:

    Mosaic analysis with a repressible cell marker for studies of gene function in neuronal morphogenesis. Lee T, Luo L. Neuron. 1999 Mar . 22(3):451-61. 10.1016/S0896-6273(00)80701-1 PubMed 10197526