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Tubp-Gal4 in pCaSpeR4
(Plasmid #17747)

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Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 17747 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    GAL4
  • Alt name
    Tubp-GAL4
  • Alt name
    GAL4 hsp70 polyA
  • Alt name
    hsp70 polyA
  • Species
    S. cerevisiae (budding yeast)
  • Insert Size (bp)
    5774
  • GenBank ID
    GAL4
  • Entrez Gene
    GAL4 (a.k.a. YPL248C, GAL81)
  • Tags / Fusion Proteins
    • Tubulin 1alpha promoter (N terminal on insert)
    • hsp70 PolyA (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site NotI, BamHI? (not destroyed)
  • 5′ sequencing primer n/a
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Tubulin 1alpha promoter fragment from Gary Struhl
  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry

Depositor Comments

The GAL4 gene containing the hsp70 polyA region was cut out with NotI from pGaTN (Brand and Perrimon, 1993) and inserted into pCaSpeR4 carrying the tubulin 1alpha promoter.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Tubp-Gal4 in pCaSpeR4 was a gift from Liqun Luo (Addgene plasmid # 17747 ; http://n2t.net/addgene:17747 ; RRID:Addgene_17747)
  • For your References section:

    Mosaic analysis with a repressible cell marker for studies of gene function in neuronal morphogenesis. Lee T, Luo L. Neuron. 1999 Mar . 22(3):451-61. 10.1016/S0896-6273(00)80701-1 PubMed 10197526