170,492 results
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Plasmid#192269PurposeDonor template for CRISPR Genome EditingDepositorInsertMCS within donor template
UseCRISPRAvailable SinceDec. 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pIF3
Plasmid#196068PurposeExpresses dCas9-Mxi1 with MoRP27 promoter and express gRNA with MoU3 promtoer in M. oryzaeDepositorInsertdCas9-Mxi1
UseCRISPRPromoterMoRP27 promoterAvailable SinceFeb. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET-6xHis-GFP(+6)
Plasmid#199160Purposeused to express a supercharged GFP variant in E. coliDepositorInsertGFP(+6)
TagsMGHHHHHHGGAMutationE16R, D86K, D112K, D127R, I138R, D207R, N222K, L2…PromoterT7Available SinceMay 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pmiRFP713-tubulin
Plasmid#136561PurposeHuman alpha-tubulin labeling with a near-infrared fluorescent protein miRFP713DepositorInsertmiRFP713-tubulin
TagsmiRFP713ExpressionMammalianPromoterCMVAvailable SinceFeb. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
U6-PGK-hCD2
Plasmid#224294PurposeExpresses sgRNA from U6 promoter and human CD2 under PGKDepositorInsertU6 sgRNA cassette with CD2 under PGK promoter (CD2 Human)
UseCRISPR and RetroviralExpressionMammalianPromoterU6/PGKAvailable SinceMarch 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
TFORF2934
Plasmid#144410PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorAvailable SinceSept. 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAG79-attL1-pegRNA-attL2
Plasmid#213050PurposeModular Unit carrying pegRNA and ngRNA scaffolds flanked by attL1 and attL2DepositorInsertsgRNA
ExpressionBacterialPromoter35S-CmYCLV-AtU6Available SinceFeb. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
spNW80
Plasmid#173487PurposeExpresses spNW80 in Bl21DepositorInsertspNW80
TagsHistagExpressionBacterialAvailable SinceSept. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCI-FLAG-mTUT4
Plasmid#60041PurposeMammalian expression of FLAG-tagged mouse TUT4DepositorAvailable SinceNov. 12, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-T1ER
Plasmid#47928PurposeT1ER is a calcium sensor targeted to ERDepositorInsertT1ER
ExpressionMammalianMutationKDELPromoterCMVAvailable SinceOct. 4, 2013AvailabilityAcademic Institutions and Nonprofits only -
pD649-HAsp-PVR-Fc(DAPA)-AviTag-6xHis
Plasmid#156765PurposeMammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.DepositorInsertPVR (PVR Human)
TagsFc(DAPA)-AviTag-6xHisExpressionMammalianMutationFc region of human IgG contains D265A and P329A m…PromoterCMV/SP6Available SinceMarch 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pX459-HypaCas9-ANLN_sgRNA
Plasmid#183874PurposepX459V2.0-HypaCas9 plasmid with ANLN sgRNA for N-terminal tagging of anillin in human cells.DepositorAvailable SinceMay 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
3922-48
Plasmid#176497PurposepTRE-EGFP-T2A-TFEB(wt)-polyADepositorInsertEGFP-T2A-TFEB(wild-type; full-length; human)
ExpressionMammalianPromoterTREAvailable SinceNov. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmG3BP_G
Plasmid#146349PurposeInsect Expression of DmG3BPDepositorInsertDmG3BP (rin Fly)
ExpressionInsectMutationV632G compared to AF231031.1, exact match to NP_5…Available SinceJan. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-hM3D(Gq)-mCherry
Plasmid#50474PurposeGq-coupled hM3D DREADD fused with mCherry under the control of human synapsin promoterDepositorHas ServiceAAV Retrograde, AAV1, AAV2, AAV5, AAV8, and AAV9InserthM3D(Gq)-mCherry (CHRM3 Human)
UseAAVTagsmCherryMutationSee supplemental documents for DREADD mutationsPromoterhuman Synapsin 1Available SinceApril 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCH45 (multiAsCas12a-KRAB lenti)
Plasmid#217330PurposeLentiviral expression of multiAsCas12a-KRABDepositorInsertmultiAsCas12a
UseCRISPR and LentiviralTags6xMycNLS and HA-SV40NLS-XTEN80-KRAB-P2A-TagBFP2MutationR1226A/E174R/S542R/K548RPromoterUCOE-SFFVAvailable SinceMarch 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV.CAG.(mito).iATPSnFR2.A95A.A119L.HaloTag
Plasmid#209722PurposeExpresses mitochondrially targeted low affinity ratiometric ATP sensorDepositorInsert(mito).iATPSnFR2.A95A.A119L.HaloTag
UseAAVExpressionMammalianPromoterCAGAvailable SinceApril 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
R777-E139 Hs.NF1
Plasmid#70423PurposeGateway ORF clone of codon optimized human NF1 [NM_000267.3] with stop codon (for native or N-terminal fusions)DepositorInsertNF1 (NF1 Human)
UseGateway entry cloneAvailable SinceMarch 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
pD649-HAsp-SIGLEC5-COMP5AP-AviTag-9xHis
Plasmid#157417PurposeMammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.DepositorAvailable SinceMarch 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pOSV804
Plasmid#126597Purposemodular integrative vector for the assembly of DNA fragments and expression in Streptomyces speciesDepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterialAvailable SinceAug. 1, 2019AvailabilityAcademic Institutions and Nonprofits only