163,606 results

1. Tet-pLKO-puro-SOX2

   Plasmid

   #47540

   Purpose

   Tet inducible knockdown of SOX2.

   Depositor

   Charles Rudin

   Article

   Rudin et al Nat Genet. 2012 Oct;44(10):1111-6. doi:

   Insert

   SOX2 shRNA
   

   Use

   Lentiviral and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   H1

   Available Since

   April 28, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3.2 TDP-43 NLS1, 5F-L YFP

   Plasmid

   #84913

   Purpose

   Mammalian expresion of TDP-43 NLS1, 5F-L YFP

   Depositor

   Aaron Gitler

   Article

   Elden et al Nature. 2010 Aug 26;466(7310):1069-75. d

   Insert

   TDP-43 (TARDBP Human)
   

   Use

   Tags

   YFP

   Expression

   Mammalian

   Mutation

   K82A, R83A, K84A, F147L, F149L, F194L, F229L, F23…

   Promoter

   CMV

   Available Since

   Nov. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. EGFP-RILP

   Plasmid

   #110498

   Purpose

   Fluorescent RILP

   Depositor

   Terence Dermody, Bernardo Mainou

   Article

   Mainou et al J Virol. 2012 Aug;86(16):8346-58. doi: 1

   Insert

   RILP (RILP Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   May 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3-FlipCherry-T2A-GFP

   Plasmid

   #124436

   Purpose

   Expresses FlipCherry (TEV cleavage sequence) and T2A GFP in mammalian cells

   Depositor

   Xiaokun Shu

   Article

   Zhang et al J Am Chem Soc. 2019 Mar 1. doi: 10.1021/

   Insert

   FlipCherry-T2A-GFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   April 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMRX-IB-pSu9-HaloTag7-mGFP

   Plasmid

   #184905

   Purpose

   Stable expression of pSu9-HaloTag7-mGFP in mammalian cells to measure mitophagy activity

   Depositor

   Noboru Mizushima

   Article

   Yim et al Elife. 2022 Aug 8;11. pii: 78923. doi: 1

   Insert

   Presequence of Fo-ATPase subunit 9
   

   Use

   Tags

   HaloTag7 and monomeric EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   June 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pYTK-DN1

   Plasmid

   #180282

   Purpose

   Cas9 cassette (TU1)

   Depositor

   Andreas Milias-Argeitis

   Article

   Novarina et al STAR Protoc. 2022 Jun 10;3(2):101358. do

   Insert

   ConLS - pPGK1 - Cas9 - tPGK1 - ConR1
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   pPGK1

   Available Since

   June 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3-S33Y Beta-catenin

   Plasmid

   #19286

   Purpose

   Mammalian expression of human beta-catenin with Flag tag and S33Y mutation

   Depositor

   Eric Fearon

   Article

   Kolligs et al Mol Cell Biol. 1999 Aug . 19(8):5696-706

   Insert

   beta-catenin (CTNNB1 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   S33Y (presumptive GSK3β phosphorylation site)

   Promoter

   Available Since

   Sept. 15, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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8. PB-gRNA-Puro

   Plasmid

   #121121

   Purpose

   Expression of a gRNA together with a Puromycin resistance

   Depositor

   Pablo Navarro

   Article

   Heurtier et al Nat Commun. 2019 Mar 7;10(1):1109. doi:

   Insert

   gRNA
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   May 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pHR-PD-1 (Y223F, Y248F)-mGFP

   Plasmid

   #180790

   Purpose

   Expression of human PD-1 (Y223F, Y248F) fused to mEGFP

   Depositor

   Enfu Hui

   Article

   Xu et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

   Insert

   PD-1 (Y223F, Y248F) (PDCD1 Human)
   

   Use

   Lentiviral

   Tags

   mEGFP

   Expression

   Mutation

   PD-1 (Y223F, Y248F)

   Promoter

   SFFV

   Available Since

   March 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pEGFP-VSP4-E228Q

    Plasmid

    #80351

    Purpose

    Expression of GFP-tagged, dominant negative VPS4 mutant

    Depositor

    Wesley Sundquist

    Article

    Votteler et al Nature. 2016 Dec 8;540(7632):292-295. do

    Insert

    VPS4-E228Q (VPS4A Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Jan. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. L13-Arl13bGFP

    Plasmid

    #40879

    Purpose

    3rd generation transfer vector

    Depositor

    Tamara Caspary

    Article

    Larkins et al Mol Biol Cell. 2011 Dec;22(23):4694-703.

    Insert

    Arl13b (Arl13b Mouse)
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Oct. 29, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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12. T777T

    Plasmid

    #113082

    Purpose

    Enhanced RNAi vektor for C. elegans. Contains two T7 polymerase termination sequences adjacent to the T7 promoter sites and a lacZ coding sequence.

    Depositor

    Tibor Vellai

    Article

    Sturm et al Nucleic Acids Res. 2018 Jun 19. pii: 504

    Type

    Empty backbone

    Use

    RNAi

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Sept. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pYTK-DN2

    Plasmid

    #180283

    Purpose

    Spacer cassette (TU3)

    Depositor

    Andreas Milias-Argeitis

    Article

    Novarina et al STAR Protoc. 2022 Jun 10;3(2):101358. do

    Insert

    ConL2 - Spacer - ConRE
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    June 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pBAMD1-4

    Plasmid

    #61565

    Purpose

    Standardized mini-Tn5 delivery plasmid, streptomycin/spectinomycin resistant

    Depositor

    Víctor de Lorenzo

    Article

    Martinez-Garcia et al Front Bioeng Biotechnol. 2014 Oct 28;2:4

    Type

    Empty backbone

    Use

    Synthetic Biology; Mini-tn5 vector

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Jan. 7, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pQTEV- PRDX2

    Plasmid

    #31338

    Depositor

    Konrad Buessow

    Article

    Bussow et al Microb Cell Fact. 2005 Jul 5;4:21. doi:

    Insert

    PRDX2 (PRDX2 Human)
    

    Use

    Tags

    His and TEV

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    March 22, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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16. pGCC4

    Plasmid

    #37058

    Depositor

    Hank Seifert

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    June 13, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCDH-CMV-CD19 puro

    Plasmid

    #196634

    Purpose

    Express CD19

    Depositor

    Nicolas Manel

    Article

    Jeremiah et al J Exp Med. 2023 May 1;220(5):e20220666.

    Insert

    CD19 (CD19 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    WT

    Promoter

    Available Since

    Feb. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMH-Halo tag

    Plasmid

    #154144

    Purpose

    Gateway compatible mammalian expression plasmid for halo tag fusion proteins

    Depositor

    Michael Huen

    Type

    Empty backbone

    Use

    Tags

    Halo tag

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCMV-SpCas9(D10A)-T2A-EGFP

    Plasmid

    #221230

    Purpose

    Express nCas9 nickase (D10) with GFP reporter

    Depositor

    Fei Chen

    Article

    Chen et al Science. 2024 Oct 11;386(6718):eadn5876.

    Insert

    SpCas9(D10A)-T2A-EGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    D10A

    Promoter

    Available Since

    Feb. 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA3.1 NPY pHLeon

    Plasmid

    #235176

    Purpose

    Plasmid expressing NPY tag with pH sensor, pHLeon (pH-Lemon with mNeonGreen), in mammalian cells

    Depositor

    Kate White

    Article

    Lin et al ACS Sens. 2024 Nov 22;9(11):6032-6039. d

    Insert

    NPY-pHLeon (NPY Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    May 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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