A genetically encoded tag for correlated light and electron microscopy of intact cells, tissues, and organisms.
Shu X, Lev-Ram V, Deerinck TJ, Qi Y, Ramko EB, Davidson MW, Jin Y, Ellisman MH, Tsien RY
PLoS Biol. 2011 Apr;9(4):e1001041. doi: 10.1371/journal.pbio.1001041. Epub 2011 Apr 5. PubMed Article
Shu X, Lev-Ram V, Deerinck TJ, Qi Y, Ramko EB, Davidson MW, Jin Y, Ellisman MH, Tsien RY
PLoS Biol. 2011 Apr;9(4):e1001041. doi: 10.1371/journal.pbio.1001041. Epub 2011 Apr 5. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 54801 | mCherry-miniSOG-C1 | Localization: C1 Cloning Vector, Excitation: 448 / 473, Emission: 500 / 528 |
| 54802 | mCherry-miniSOG-Display-5 | Localization: Cytoplasm, Excitation: 448 / 473, Emission: 500 / 528 |
| 54803 | mCherry-miniSOG-N1 | Localization: N1 Cloning Vector, Excitation: 448 / 473, Emission: 500 / 528 |
| 54804 | mCherry-miniSOG-Peroxisomes-2 | Localization: Peroxisomes, Excitation: 448 / 473, Emission: 500 / 528 |
| 54821 | miniSOG-C1 | Localization: C1 Cloning Vector, Excitation: 448 / 473, Emission: 500 / 528 |
| 54822 | miniSOG-Display-5 | Localization: Cytoplasm, Excitation: 448 / 473, Emission: 500 / 528 |
| 54823 | miniSOG-Lifeact-7 | Localization: Actin, Excitation: 448 / 473, Emission: 500 / 528 |
| 54824 | miniSOG-N1 | Localization: N1 Cloning Vector, Excitation: 448 / 473, Emission: 500 / 528 |
| 54846 | PA-mCherry-miniSOG-Lifeact-7 | Localization: Actin, Excitation: 448 / 473, Emission: 500 / 528 |
| 54847 | PA-mCherry-miniSOG-Peroxisomes-2 | Localization: Peroxisomes, Excitation: 448 / 473, Emission: 500 / 528 |
| 54854 | tdminiSOG-C1 | Localization: C1 Cloning Vector, Excitation: 448 / 473, Emission: 500 / 528 |
| 54855 | tdminiSOG-N1 | Localization: N1 Cloning Vector, Excitation: 448 / 473, Emission: 500 / 528 |
| 55085 | mCherry-miniSOG-Actin-7 | Localization: Actin, Excitation: 448 / 473, Emission: 500 / 528 |
| 55086 | mCherry-miniSOG-C-Src-7 | Localization: Membrane/Vesicles, Excitation: 448 / 473, Emission: 500 / 528 |
| 55087 | mCherry-miniSOG-CDC42-C-10 | Localization: Membrane, Excitation: 448 / 473, Emission: 500 / 528 |
| 55088 | mCherry-miniSOG-Clathrin-15 | Localization: Clathrin Vesicles, Excitation: 448 / 473, Emission: 500 / 528 |
| 55089 | mCherry-miniSOG-EB3-7 | Localization: MT End Binding Protein, Excitation: 448 / 473, Emission: 500 / 528 |
| 55090 | mCherry-miniSOG-H2B-C-10 | Localization: Nucleus/Histones, Excitation: 448 / 473, Emission: 500 / 528 |
| 55091 | mCherry-miniSOG-Keratin-17 | Note- This plasmid contains Keratin 18. Localization: Cytokeratin, Excitation: 448 / 473, Emission: 500 / 528 |
| 55092 | mCherry-miniSOG-Paxillin-22 | Localization: Focal Adhesions, Excitation: 448 / 473, Emission: 500 / 528 |
| 55093 | mCherry-miniSOG-Profilin-10 | Localization: Cytoskeleton, Excitation: 448 / 473, Emission: 500 / 528 |
| 55094 | mCherry-miniSOG-Rab5a-7 | Localization: Ras GTPase, Excitation: 448 / 473, Emission: 500 / 528 |
| 55095 | mCherry-miniSOG-RatLC3 | Localization: Microtubule-Binding Protein, Excitation: 448 / 473, Emission: 500 / 528 |
| 55096 | mCherry-miniSOG-Talin-N-10 | Localization: Focal Adhesions, Excitation: 448 / 473, Emission: 500 / 528 |
| 55097 | mCherry-miniSOG-Tubulin-6 | Localization: Microtubules, Excitation: 448 / 473, Emission: 500 / 528 |
| 55098 | mCherry-miniSOG-VASP-5 | Localization: Actin/Focal Adhesions, Excitation: 448 / 473, Emission: 500 / 528 |
| 55099 | mCherry-miniSOG-Vimentin-7 | Localization: Intermediate Filaments, Excitation: 448 / 473, Emission: 500 / 528 |
| 55100 | mCherry-miniSOG-Vinculin-23 | Localization: Focal Adhesions, Excitation: 448 / 473, Emission: 500 / 528 |
| 55101 | mCherry-miniSOG-Zyxin-6 | Localization: Focal Adhesions, Excitation: 448 / 473, Emission: 500 / 528 |
| 57761 | miniSOG-Actin-7 | Localization: Actin, Excitation: 448 / 473, Emission: 500 / 528 |
| 57762 | miniSOG-Alpha-Actinin-19 | Localization: Alpha Actinin, Excitation: 448 / 473, Emission: 500 / 528 |
| 57763 | miniSOG-Alpha-V-Integrin-25 | Localization: Focal Adhesions, Excitation: 448 / 473, Emission: 500 / 528 |
| 57764 | miniSOG-Clathrin-15 | Localization: Clathrin Vesicles, Excitation: 448 / 473, Emission: 500 / 528 |
| 57765 | miniSOG-EB3-7 | Localization: MT End Binding Protein, Excitation: 448 / 473, Emission: 500 / 528 |
| 57766 | miniSOG-ER-5 | Localization: Endoplasmic Reticulum, Excitation: 448 / 473, Emission: 500 / 528 |
| 57768 | miniSOG-Golgi-7 | Localization: Golgi, Excitation: 448 / 473, Emission: 500 / 528 |
| 57769 | miniSOG-H2B-C-10 | Localization: Nucleus/Histones, Excitation: 448 / 473, Emission: 500 / 528 |
| 57770 | miniSOG-Keratin-17 | Note- This plasmid contains Keratin 18. Localization: Cytokeratin, Excitation: 448 / 473, Emission: 500 / 528 |
| 57771 | miniSOG-LaminB1-10 | Localization: Nuclear Envelope, Excitation: 448 / 473, Emission: 500 / 528 |
| 57772 | miniSOG-MAP4-C-10 | Localization: Microtubules, Excitation: 448 / 473, Emission: 500 / 528 |
| 57773 | miniSOG-Mito-7 | Localization: Mitochondria, Excitation: 448 / 473, Emission: 500 / 528 |
| 57774 | miniSOG-Rab5a-7 | Localization: Ras GTPase, Excitation: 448 / 473, Emission: 500 / 528 |
| 57775 | miniSOG-RatLC3 | Localization: Microtubule-Binding Protein, Excitation: 448 / 473, Emission: 500 / 528 |
| 57776 | miniSOG-Talin-C-10 | Localization: Focal Adhesions, Excitation: 448 / 473, Emission: 500 / 528 |
| 57777 | miniSOG-Tubulin-6 | Localization: Microtubules, Excitation: 448 / 473, Emission: 500 / 528 |
| 57778 | miniSOG-VASP-5 | Localization: Actin/Focal Adhesions, Excitation: 448 / 473, Emission: 500 / 528 |
| 57779 | miniSOG-Vimentin-7 | Localization: Intermediate Filaments, Excitation: 448 / 473, Emission: 500 / 528 |
| 57780 | miniSOG-Vinculin-23 | Localization: Focal Adhesions, Excitation: 448 / 473, Emission: 500 / 528 |
| 57781 | miniSOG-Zyxin-6 | Localization: Focal Adhesions, Excitation: 448 / 473, Emission: 500 / 528 |
| 57782 | PA-mCherry-miniSOG-Alpha-Actinin-19 | Localization: Alpha Actinin, Excitation: 448 / 473, Emission: 500 / 528 |
| 57784 | PA-mCherry-miniSOG-CAF1-C-10 | Localization: Chromatin Assembly Factor 1, Excitation: 448 / 473, Emission: 500 / 528 |
| 57785 | PA-mCherry-miniSOG-C-Src-7 | Localization: Membrane/Vesicles, Excitation: 448 / 473, Emission: 500 / 528 |
| 57786 | PA-mCherry-miniSOG-Cx26-7 | Localization: Gap Junctions, Excitation: 448 / 473, Emission: 500 / 528 |
| 57787 | PA-mCherry-miniSOG-Cx43-7 | Localization: Gap Junctions, Excitation: 448 / 473, Emission: 500 / 528 |
| 57788 | PA-mCherry-miniSOG-EB3-7 | Localization: MT End Binding Protein, Excitation: 448 / 473, Emission: 500 / 528 |
| 57789 | PA-mCherry-miniSOG-H2B-C-10 | Localization: Nucleus/Histones, Excitation: 448 / 473, Emission: 500 / 528 |
| 57791 | PA-mCherry-miniSOG-Golgi-7 | Localization: Golgi, Excitation: 448 / 473, Emission: 500 / 528 |
| 57792 | PA-mCherry-miniSOG-Keratin-17 | Note- This plasmid contains Keratin 18. Localization: Cytokeratin, Excitation: 448 / 473, Emission: 500 / 528 |
| 57793 | PA-mCherry-miniSOG-Lysosomes-20 | Localization: Lysosome Membrane, Excitation: 448 / 473, Emission: 500 / 528 |
| 57794 | PA-mCherry-miniSOG-MAP4-C-10 | Localization: Microtubules, Excitation: 448 / 473, Emission: 500 / 528 |
| 57795 | PA-mCherry-miniSOG-Mito-7 | Localization: Mitochondria, Excitation: 448 / 473, Emission: 500 / 528 |
| 57796 | PA-mCherry-miniSOG-Paxillin-22 | Localization: Focal Adhesions, Excitation: 448 / 473, Emission: 500 / 528 |
| 57797 | PA-mCherry-miniSOG-Talin-N-10 | Localization: Focal Adhesions, Excitation: 448 / 473, Emission: 500 / 528 |
| 57799 | PA-mCherry-miniSOG-VE-Cadherin-N-10 | Localization: Tight Junctions, Excitation: 448 / 473, Emission: 500 / 528 |
| 57800 | PA-mCherry-miniSOG-Vimentin-7 | Localization: Intermediate Filaments, Excitation: 448 / 473, Emission: 500 / 528 |
| 57801 | PA-mCherry-miniSOG-Vinculin-23 | Localization: Focal Adhesions, Excitation: 448 / 473, Emission: 500 / 528 |
| 57802 | PA-mCherry-miniSOG-Zyxin-6 | Localization: Focal Adhesions, Excitation: 448 / 473, Emission: 500 / 528 |
| 57803 | tdminiSOG-Actin-7 | Localization: Actin, Excitation: 448 / 473, Emission: 500 / 528 |
| 57804 | tdminiSOG-H2B-C-10 | Localization: Nucleus/Histones, Excitation: 448 / 473, Emission: 500 / 528 |
| 57805 | tdminiSOG-MAP4-C-10 | Localization: Microtubules, Excitation: 448 / 473, Emission: 500 / 528 |
| 57806 | tdminiSOG-PCNA-19 | Localization: Nucleus, Excitation: 448 / 473, Emission: 500 / 528 |
| 57807 | tdminiSOG-RatLC3 | Localization: Microtubule-Binding Protein, Excitation: 448 / 473, Emission: 500 / 528 |
| 57808 | tdminiSOG-Tubulin-6 | Localization: Microtubules, Excitation: 448 / 473, Emission: 500 / 528 |
| 91774 | pBAD miniSOG | Bacterial expression of a fluorescent protein, mini Singlet Oxygen Generator (miniSOG) |