|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||25919||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4700
Vector typeMammalian Expression
Growth in Bacteria
Bacterial Resistance(s)Kanamycin, 50 μg/mL
Copy numberHigh Copy
Alt nameFKBP12-Rapamycin Binding domain of mTOR
SpeciesH. sapiens (human)
Insert Size (bp)360
Entrez GeneMTOR (a.k.a. FRAP, FRAP1, FRAP2, RAFT1, RAPT1, SKS)
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site BamHI (not destroyed)
- 5′ sequencing primer CMV-Fw (Common Sequencing Primers)
Articles Citing this Plasmid
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
FRB is a small domain derived from full length mTOR. In brief, rapamycin binds to a protein called FKBP12. Rapamycin bound to FKBP12 inhibits mTOR signaling
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pEGFP-FRB was a gift from Klaus Hahn (Addgene plasmid # 25919 ; http://n2t.net/addgene:25919 ; RRID:Addgene_25919)
For your References section:Engineered allosteric activation of kinases in living cells. Karginov AV, Ding F, Kota P, Dokholyan NV, Hahn KM. Nat Biotechnol. 2010 Jun 27. ():. 10.1038/nbt.1639 PubMed 20581846