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T7-RelA
(Plasmid #21984)

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Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 21984 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pEv3s
  • Backbone manufacturer
    Matthias P et al. Nucleic Acids Research (1989). 17(15): 6418
  • Backbone size w/o insert (bp) 5120
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    NFkB p65 subunit
  • Alt name
    RelA
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1600
  • Mutation
    For more details for this expression construct, as well as associated mutants, please contact: Dr. Lin-Feng Chen ([email protected])
  • Entrez Gene
    RELA (a.k.a. CMCU, NFKB3, p65)
  • Tag / Fusion Protein
    • T7 gp10 (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site XbaI (not destroyed)
  • 5′ sequencing primer NTer primer 5'-GGGGGATCTTGGTGGCGTG-3'
  • 3′ sequencing primer CTer primer: 5'-CCCTGAAAACTTTGCCCCCTCC-3'
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry
  • Articles Citing this Plasmid
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    T7-RelA was a gift from Warner Greene (Addgene plasmid # 21984 ; http://n2t.net/addgene:21984 ; RRID:Addgene_21984)
  • For your References section:

    Duration of nuclear NF-kappaB action regulated by reversible acetylation. Chen Lf , Fischle W, Verdin E, Greene WC. Science. 2001 Aug 31. 293(5535):1653-7. 10.1126/science.1062374 PubMed 11533489